Eph receptor A5 (EPHA5) (NM_004439) Human Tagged ORF Clone

CAT#: RC600042

EPHA5 (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens EPH receptor A5, transcript variant 1, Signal peptide (1-24) plus EC domain (25-573)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: DDK-His
• Symbol: Eph receptor A5
• Synonyms: CEK7; EHK-1; EHK1; EK7; HEK7; TYRO4
• Vector: pCMV6-XL5-DDK-His
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC600042 representing leader sequence plus the extracellular domain region of NM_004439
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGTCGACTGGATCTGGTACCGAGGAGATCCGCCGCCG
  CGATCGCC
  
  ATGCGGGGCTCGGGGCCCCGGGGTGCGGGACGCCGGCGGCCCCCAAGCGGCGGCGGCGACACCCCCATCA
  CCCCAGCGTCCCTGGCCGGCTGCTACTCTGCACCTCGACGGGCTCCCCTCTGGACGTGCCTTCTCCTGTG
  CGCCGCACTCCGGACCCTCCTGGCCAGCCCCAGCAACGAAGTGAATTTATTGGATTCACGCACTGTCATG
  GGGGACCTGGGATGGATTGCTTTTCCAAAAAATGGGTGGGAAGAGATTGGTGAAGTGGATGAAAATTATG
  CCCCTATCCACACATACCAAGTATGCAAAGTGATGGAACAGAATCAGAATAACTGGCTTTTGACCAGTTG
  GATCTCCAATGAAGGTGCTTCCAGAATCTTCATAGAACTCAAATTTACCCTGCGGGACTGCAACAGCCTT
  CCTGGAGGACTGGGGACCTGTAAGGAAACCTTTAATATGTATTACTTTGAGTCAGATGATCAGAATGGGA
  GAAACATCAAGGAAAACCAATACATCAAAATTGATACCATTGCTGCCGATGAAAGCTTTACAGAACTTGA
  TCTTGGTGACCGTGTTATGAAACTGAATACAGAGGTCAGAGATGTAGGACCTCTAAGCAAAAAGGGATTT
  TATCTTGCTTTTCAAGATGTTGGTGCTTGCATTGCTCTGGTTTCTGTGCGTGTATACTATAAAAAATGCC
  CTTCTGTGGTACGACACTTGGCTGTCTTCCCTGACACCATCACTGGAGCTGATTCTTCCCAATTGCTCGA
  AGTGTCAGGCTCCTGTGTCAACCATTCTGTGACCGATGAACCTCCCAAAATGCACTGCAGCGCCGAAGGG
  GAGTGGCTGGTGCCCATCGGGAAATGCATGTGCAAGGCAGGATATGAAGAGAAAAATGGCACCTGTCAAG
  TGTGCAGACCTGGGTTCTTCAAAGCCTCACCTCACATCCAGAGCTGCGGCAAATGTCCACCTCACAGTTA
  TACCCATGAGGAAGCTTCAACCTCTTGTGTCTGTGAAAAGGATTATTTCAGGAGAGAGTCTGATCCACCC
  ACAATGGCATGCACAAGACCCCCCTCTGCTCCTCGGAATGCCATCTCAAATGTTAATGAAACTAGTGTCT
  TTCTGGAATGGATTCCGCCTGCTGACACTGGTGGAAGGAAAGACGTGTCATATTATATTGCATGCAAGAA
  GTGCAACTCCCATGCAGGTGTGTGTGAGGAGTGTGGCGGTCATGTCAGGTACCTTCCCCGGCAAAGCGGC
  CTGAAAAACACCTCTGTCATGATGGTGGATCTACTCGCTCACACAAACTATACCTTTGAGATTGAGGCAG
  TGAATGGAGTGTCCGACTTGAGCCCAGGAGCCCGGCAGTATGTGTCTGTAAATGTAACCACAAATCAAGC
  AGCTCCATCTCCAGTCACCAATGTGAAAAAAGGGAAAATTGCAAAAAACAGCATCTCTTTGTCTTGGCAA
  GAACCAGATCGTCCCAATGGAATCATCCTAGAGTATGAAATCAAGTATTTTGAAAAGGACCAAGAGACCA
  GCTACACGATTATCAAATCTAAAGAGACAACTATTACTGCAGAGGGCTTGAAACCAGCTTCAGTTTATGT
  CTTCCAAATTCGAGCACGTACAGCAGCAGGCTATGGTGTCTTCAGTCGAAGATTTGAGTTTGAAACCACC
  CCAGTGTTTGCAGCATCCAGCGATCAAAGCCAGATTCCT
  
  ACGCGTTCAGGCGACTACAAGGATGACGACGATAAGGGATCTCATCATCACCATCACCATTAATGAGATC
  TGGTACCGATATCAAGCTTGTCGACTCTAGA
  Protein Sequence

  >RC600042 representing signal peptide plus the extracellular domain region of NM_004439
  Red=Cloning sites Green= DDK and 6XHIS Tags
  
  MRGSGPRGAGRRRPPSGGGDTPITPASLAGCYSAPRRAPLWTCLLLCAALRTLLASPSNEVNLLDSRTVM
  GDLGWIAFPKNGWEEIGEVDENYAPIHTYQVCKVMEQNQNNWLLTSWISNEGASRIFIELKFTLRDCNSL
  PGGLGTCKETFNMYYFESDDQNGRNIKENQYIKIDTIAADESFTELDLGDRVMKLNTEVRDVGPLSKKGF
  YLAFQDVGACIALVSVRVYYKKCPSVVRHLAVFPDTITGADSSQLLEVSGSCVNHSVTDEPPKMHCSAEG
  EWLVPIGKCMCKAGYEEKNGTCQVCRPGFFKASPHIQSCGKCPPHSYTHEEASTSCVCEKDYFRRESDPP
  TMACTRPPSAPRNAISNVNETSVFLEWIPPADTGGRKDVSYYIACKKCNSHAGVCEECGGHVRYLPRQSG
  LKNTSVMMVDLLAHTNYTFEIEAVNGVSDLSPGARQYVSVNVTTNQAAPSPVTNVKKGKIAKNSISLSWQ
  EPDRPNGIILEYEIKYFEKDQETSYTIIKSKETTITAEGLKPASVYVFQIRARTAAGYGVFSRRFEFETT
  PVFAASSDQSQIP
  
  TRSGTRSGDYKDDDDKGSHHHHHH
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_004439
• ORF Size: 1719 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the extra cellular domain of the protein with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004439.6, NP_004430.4
• RefSeq Size: 8418 bp
• RefSeq ORF: 3114 bp
• Locus ID: 2044
• UniProt ID: P54756
• Cytogenetics: 4q13.1-q13.2
• Domains: pkinase, EPH_lbd, TyrKc, SAM, S_TKc, FN3
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Axon guidance
• MW: 63.2 kDa
• Gene Summary: This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013]