DTX4 (NM_001300727) Human Tagged ORF Clone

CAT#: RC238652

DTX4 (myc-DDK-tagged) - Human deltex 4, E3 ubiquitin ligase (DTX4), transcript variant 2

Product Images

Circular map for RC238652

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DTX4
• Synonyms: RNF155
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238652 representing NM_001300727
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGTGGGCATCACCATCCAGCATGCCTATGAGAAGCAGCACCCCTGGATCGACCTCACTTCCATTG
  GCTTTAGCTACGTAATTGACTTCAACACCATGGGCCAGATCAACCGTCAGACCCAGCGCCAACGCCGCGT
  CCGCCGGCGCCTCGACCTCATCTACCCCATGGTCACAGGGACCTTGCCTAAGGCTCAGTCCTGGCCAGTC
  AGCCCTGGGCCAGCCACCTCGCCCCCCATGTCCCCCTGCTCCTGTCCCCAGTGTGTCTTGGTGATGAGTG
  TTAAGGCAGCCGTGGTCAATGGCAGCACTGGGCCCCTACAGCTGCCAGTGACCCGCAAGAACATGCCGCC
  TCCTGGAGTGGTCAAGCTACCCCCACTGCCAGGCTCTGGGGCCAAGCCACTGGACAGCACAGGCACCATT
  CGAGGCCCACTGAAGACCGCCCCATCGCAGGTGATCCGGAGACAAGCCTCCAGCATGCCCACTGGGACAA
  CCATGGGCTCTCCTGCCAGTCCCCCAGGACCCAACAGCAAGACCGGAAGGGTGGCCCTGGCCACCTTGAA
  TCGTACCAACCTGCAGCGACTGGCCATTGCCCAGTCCCGGGTGCTGATCGCCTCTGGGGTCCCCACAGTC
  CCAGTGAAGAACCTAAATGGGTCCAGTCCTGTCAACCCTGCCTTGGCAGGAATCACTGGGATCCTCATGA
  GTGCAGCGGGGCTGCCTGTGTGTCTCACCAGGCCACCAAAGCTGGTCCTACACCCACCCCCCGTCAGCAA
  GAGTGAAATAAAATCCATCCCAGGGGTTTCCAACACAAGCCGCAAGACCACCAAAAAACAAGCCAAGAAA
  GGTAAAACCCCAGAGGAAGTGCTAAAAAAATATCTACAGAAAGTCCGGCACCCACCAGATGAGGACTGCA
  CCATCTGTATGGAACGCCTCACGGCCCCCTCAGGCTACAAGGGCCCGCAGCCTACGGTAAAACCTGACCT
  GGTAGGGAAGCTGTCCAGATGCGGCCACGTCTACCACATCTACTGCTTGGTTGCCATGTACAACAATGGG
  AACAAGGATGGAAGTTTGCAGTGTCCAACCTGCAAGACCATTTATGGGGTGAAGACAGGCACCCAACCTC
  CAGGGAAGATGGAGTACCACCTCATCCCCCACTCCTTGCCTGGCCACCCAGACTGCAAAACCATCCGGAT
  CATCTACAGCATCCCCCCCGGCATTCAGGGACCGGAACACCCGAATCCTGGGAAGAGTTTCAGCGCCCGA
  GGCTTCCCACGACACTGTTACCTTCCGGACAGCGAGAAAGGGAGAAAAGTTCTGAAGCTGCTGCTCGTGG
  CCTGGGATCGCCGCCTCATTTTTGCCATTGGCACCTCCAGCACCACAGGCGAGTCAGACACCGTCATCTG
  GAATGAGGTCCACCACAAGACAGAGTTTGGCTCTAATCTCACTGGCCATGGCTACCCAGATGCCAATTAC
  CTGGATAATGTGCTGGCTGAACTGGCTGCCCAGGGCATCTCTGAGGACAGCACTGCCCAGGAGAAGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238652 representing NM_001300727
  Red=Cloning site Green=Tags(s)
  
  MEVGITIQHAYEKQHPWIDLTSIGFSYVIDFNTMGQINRQTQRQRRVRRRLDLIYPMVTGTLPKAQSWPV
  SPGPATSPPMSPCSCPQCVLVMSVKAAVVNGSTGPLQLPVTRKNMPPPGVVKLPPLPGSGAKPLDSTGTI
  RGPLKTAPSQVIRRQASSMPTGTTMGSPASPPGPNSKTGRVALATLNRTNLQRLAIAQSRVLIASGVPTV
  PVKNLNGSSPVNPALAGITGILMSAAGLPVCLTRPPKLVLHPPPVSKSEIKSIPGVSNTSRKTTKKQAKK
  GKTPEEVLKKYLQKVRHPPDEDCTICMERLTAPSGYKGPQPTVKPDLVGKLSRCGHVYHIYCLVAMYNNG
  NKDGSLQCPTCKTIYGVKTGTQPPGKMEYHLIPHSLPGHPDCKTIRIIYSIPPGIQGPEHPNPGKSFSAR
  GFPRHCYLPDSEKGRKVLKLLLVAWDRRLIFAIGTSSTTGESDTVIWNEVHHKTEFGSNLTGHGYPDANY
  LDNVLAELAAQGISEDSTAQEKD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001300727
• ORF Size: 1539 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001300727.1, NP_001287656.1
• RefSeq Size: 5386 bp
• RefSeq ORF: 1542 bp
• Locus ID: 23220
• UniProt ID: Q9Y2E6
• Cytogenetics: 11q12.1
• Protein Families: Druggable Genome
• Protein Pathways: Notch signaling pathway
• MW: 55.7 kDa
• Gene Summary: Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations (By similarity). Functions as a ubiquitin ligase protein in vivo, mediating 'Lys48'-linked polyubiquitination and promoting degradation of TBK1, targeting to TBK1 requires interaction with NLRP4.[UniProtKB/Swiss-Prot Function]