DTX4 (NM_001300727) Human Tagged ORF Clone

CAT#: RG238652

DTX4 (tGFP-tagged) - Human deltex 4, E3 ubiquitin ligase (DTX4), transcript variant 2

Product Images

Circular map for RG238652

Specifications

Product Data

• Tag: TurboGFP
• Symbol: DTX4
• Synonyms: RNF155
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238652 representing NM_001300727.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAAGTGGGCATCACCATCCAGCATGCCTATGAGAAGCAGCACCCCTGGATCGACCTCACTTCCATT
  GGCTTTAGCTACGTAATTGACTTCAACACCATGGGCCAGATCAACCGTCAGACCCAGCGCCAACGCCGC
  GTCCGCCGGCGCCTCGACCTCATCTACCCCATGGTCACAGGGACCTTGCCTAAGGCTCAGTCCTGGCCA
  GTCAGCCCTGGGCCAGCCACCTCGCCCCCCATGTCCCCCTGCTCCTGTCCCCAGTGTGTCTTGGTGATG
  AGTGTTAAGGCAGCCGTGGTCAATGGCAGCACTGGGCCCCTACAGCTGCCAGTGACCCGCAAGAACATG
  CCGCCTCCTGGAGTGGTCAAGCTACCCCCACTGCCAGGCTCTGGGGCCAAGCCACTGGACAGCACAGGC
  ACCATTCGAGGCCCACTGAAGACCGCCCCATCGCAGGTGATCCGGAGACAAGCCTCCAGCATGCCCACT
  GGGACAACCATGGGCTCTCCTGCCAGTCCCCCAGGACCCAACAGCAAGACCGGAAGGGTGGCCCTGGCC
  ACCTTGAATCGTACCAACCTGCAGCGACTGGCCATTGCCCAGTCCCGGGTGCTGATCGCCTCTGGGGTC
  CCCACAGTCCCAGTGAAGAACCTAAATGGGTCCAGTCCTGTCAACCCTGCCTTGGCAGGAATCACTGGG
  ATCCTCATGAGTGCAGCGGGGCTGCCTGTGTGTCTCACCAGGCCACCAAAGCTGGTCCTACACCCACCC
  CCCGTCAGCAAGAGTGAAATAAAATCCATCCCAGGGGTTTCCAACACAAGCCGCAAGACCACCAAAAAA
  CAAGCCAAGAAAGGTAAAACCCCAGAGGAAGTGCTAAAAAAATATCTACAGAAAGTCCGGCACCCACCA
  GATGAGGACTGCACCATCTGTATGGAACGCCTCACGGCCCCCTCAGGCTACAAGGGCCCGCAGCCTACG
  GTAAAACCTGACCTGGTAGGGAAGCTGTCCAGATGCGGCCACGTCTACCACATCTACTGCTTGGTTGCC
  ATGTACAACAATGGGAACAAGGATGGAAGTTTGCAGTGTCCAACCTGCAAGACCATTTATGGGGTGAAG
  ACAGGCACCCAACCTCCAGGGAAGATGGAGTACCACCTCATCCCCCACTCCTTGCCTGGCCACCCAGAC
  TGCAAAACCATCCGGATCATCTACAGCATCCCCCCCGGCATTCAGGGACCGGAACACCCGAATCCTGGG
  AAGAGTTTCAGCGCCCGAGGCTTCCCACGACACTGTTACCTTCCGGACAGCGAGAAAGGGAGAAAAGTT
  CTGAAGCTGCTGCTCGTGGCCTGGGATCGCCGCCTCATTTTTGCCATTGGCACCTCCAGCACCACAGGC
  GAGTCAGACACCGTCATCTGGAATGAGGTCCACCACAAGACAGAGTTTGGCTCTAATCTCACTGGCCAT
  GGCTACCCAGATGCCAATTACCTGGATAATGTGCTGGCTGAACTGGCTGCCCAGGGCATCTCTGAGGAC
  AGCACTGCCCAGGAGAAGGAC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238652
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MEVGITIQHAYEKQHPWIDLTSIGFSYVIDFNTMGQINRQTQRQRRVRRRLDLIYPMVTGTLPKAQSWP
  VSPGPATSPPMSPCSCPQCVLVMSVKAAVVNGSTGPLQLPVTRKNMPPPGVVKLPPLPGSGAKPLDSTG
  TIRGPLKTAPSQVIRRQASSMPTGTTMGSPASPPGPNSKTGRVALATLNRTNLQRLAIAQSRVLIASGV
  PTVPVKNLNGSSPVNPALAGITGILMSAAGLPVCLTRPPKLVLHPPPVSKSEIKSIPGVSNTSRKTTKK
  QAKKGKTPEEVLKKYLQKVRHPPDEDCTICMERLTAPSGYKGPQPTVKPDLVGKLSRCGHVYHIYCLVA
  MYNNGNKDGSLQCPTCKTIYGVKTGTQPPGKMEYHLIPHSLPGHPDCKTIRIIYSIPPGIQGPEHPNPG
  KSFSARGFPRHCYLPDSEKGRKVLKLLLVAWDRRLIFAIGTSSTTGESDTVIWNEVHHKTEFGSNLTGH
  GYPDANYLDNVLAELAAQGISEDSTAQEKD
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001300727
• ORF Size: 1539 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001300727.1, NP_001287656.1
• RefSeq Size: 5386 bp
• RefSeq ORF: 1542 bp
• Locus ID: 23220
• UniProt ID: Q9Y2E6
• Cytogenetics: 11q12.1
• Protein Families: Druggable Genome
• Protein Pathways: Notch signaling pathway
• MW: 55.7 kDa
• Gene Summary: Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations (By similarity). Functions as a ubiquitin ligase protein in vivo, mediating 'Lys48'-linked polyubiquitination and promoting degradation of TBK1, targeting to TBK1 requires interaction with NLRP4.[UniProtKB/Swiss-Prot Function]