CACNA2D2 (NM_001174051) Human Tagged ORF Clone

CAT#: RC230662

CACNA2D2 (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, alpha 2/delta subunit 2 (CACNA2D2), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY433661) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC230662 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CACNA2D2 protein (Cat# TP330662). The protein was produced from HEK293T cells transfected with CACNA2D2 cDNA clone (Cat# RC230662) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CACNA2D2
• Synonyms: CACNA2D; CASVDD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230662 representing NM_001174051
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGCCGGCTCGGACCTGCGGCGCCTCTCGGCCCGGCCCAGCGCGGACTGCGCGCCCCTGGCCCG
  GCTGCGGCCCCCACCCTGGCCCCGGCACCCGGCGCCCGACGTCCGGGCCCCCGCGCCCGCTGTGGCTGCT
  GCTGCCGCTTCTACCGCTGCTCGCCGCCCCCGGCGCCTCTGCCTACAGCTTCCCCCAGCAGCACACGATG
  CAGCACTGGGCCCGGCGTCTGGAGCAGGAGGTCGACGGCGTGATGCGGATTTTTGGAGGCGTCCAGCAGC
  TCCGTGAGATTTACAAGGACAACCGGAACCTGTTCGAGGTACAGGAGAATGAGCCTCAGAAGTTGGTGGA
  GAAGGTGGCAGGGGACATTGAGAGCCTTCTGGACAGGAAGGTGCAGGCCCTGAAGAGACTGGCTGATGCT
  GCAGAGAACTTCCAGAAAGCACACCGCTGGCAGGACAACATCAAGGAGGAAGACATCGTGTACTATGACG
  CCAAGGCTGACGCTGAGCTGGACGACCCTGAGAGTGAGGATGTGGAAAGGGGGTCTAAGGCCAGCACCCT
  AAGGCTGGACTTCATCGAGGACCCAAACTTCAAGAACAAGGTCAACTATTCATACGCGGCTGTACAGATC
  CCTACGGACATCTACAAAGGCTCCACTGTCATCCTCAATGAGCTCAACTGGACAGAGGCCCTGGAGAATG
  TGTTCATGGAAAACCGCAGACAAGACCCCACACTGCTGTGGCAGGTCTTCGGCAGCGCCACAGGAGTCAC
  TCGCTACTACCCGGCCACCCCGTGGCGAGCCCCCAAGAAGATCGACCTGTACGATGTCCGAAGGAGACCC
  TGGTATATCCAGGGGGCCTCGTCACCCAAAGACATGGTCATCATCGTGGATGTGAGTGGCAGTGTGAGCG
  GCCTGACCCTGAAGCTGATGAAGACATCTGTCTGCGAGATGCTGGACACGCTGTCTGATGATGACTATGT
  GAATGTGGCCTCGTTCAACGAGAAGGCACAGCCTGTGTCATGCTTCACACACCTGGTGCAGGCCAATGTG
  CGCAACAAGAAGGTGTTCAAGGAAGCTGTGCAGGGCATGGTGGCCAAGGGCACCACAGGCTACAAGGCCG
  GCTTTGAGTATGCCTTTGACCAGCTGCAGAACTCCAACATCACTCGGGCCAACTGCAACAAGATGATCAT
  GATGTTCACGGATGGTGGTGAGGACCGCGTGCAGGACGTCTTTGAGAAGTACAATTGGCCAAACCGGACG
  GTGCGCGTGTTTACTTTCTCCGTGGGGCAGCATAACTATGACGTCACACCGCTGCAGTGGATGGCCTGTG
  CCAACAAAGGCTACTATTTTGAGATCCCTTCCATCGGAGCCATCCGCATCAACACACAGGAATATCTAGA
  TGTGTTGGGCAGGCCCATGGTGCTGGCAGGCAAGGAGGCCAAGCAGGTGCAGTGGACCAACGTGTATGAG
  GATGCACTGGGACTGGGGTTGGTGGTAACAGGGACCCTCCCTGTTTTCAACCTGACACAGGATGGCCCTG
  GGGAAAAGAAGAACCAGCTGATCCTGGGCGTGATGGGCATTGACGTGGCTCTGAATGACATCAAGAGGCT
  GACCCCCAACTACACGCTTGGAGCCAACGGCTATGTGTTTGCCATTGACCTGAACGGCTACGTGTTGCTG
  CACCCCAATCTCAAGCCCCAGACCACCAACTTCCGGGAGCCTGTGACTCTGGACTTCCTGGATGCGGAGC
  TAGAGGATGAGAACAAGGAAGAGATCCGTCGGAGCATGATTGATGGCAACAAGGGCCACAAGCAGATCAG
  AACGTTGGTCAAGTCCCTGGATGAGAGGTACATAGATGAGGTGACACGGAACTACACCTGGGTGCCTATA
  AGGAGCACTAACTACAGCCTGGGGCTGGTGCTCCCACCCTACAGCACCTTCTACCTCCAAGCCAATCTCA
  GTGACCAGATCCTGCAGGTCAAGTTGCCAATCAGCAAACTGAAGGATTTTGAGTTCCTGCTCCCCAGCAG
  CTTTGAGTCTGAAGGACACGTTTTCATTGCTCCCAGAGAGTACTGCAAGGACCTGAATGCCTCAGACAAC
  AACACCGAGTTCCTGAAAAACTTTATTGAGCTCATGGAGAAAGTGACTCCAGACTCCAAGCAGTGCAACA
  ACTTCCTTCTGCACAACCTGATCTTGGACACGGGCATCACGCAGCAGCTGGTAGAGCGTGTGTGGAGGGA
  CCAGGATCTCAACACGTACAGCCTACTGGCCGTGTTCGCTGCCACAGACGGTGGCATCACCCGAGTCTTC
  CCCAACAAGGCAGCTGAGGACTGGACAGAGAACCCTGAGCCCTTCAATGCCAGCTTCTACCGCCGCAGCC
  TGGATAACCACGGTTATGTCTTCAAGCCCCCACACCAGGATGCCCTGTTAAGGCCGCTGGAGCTGGAGAA
  TGACACTGTGGGCATCCTCGTCAGCACAGCTGTGGAGCTCAGCCTAGGCAGGCGCACACTGAGGCCAGCA
  GTGGTGGGCGTCAAGCTGGACCTAGAGGCTTGGGCTGAGAAGTTCAAGGTGCTAGCCAGCAACCGTACCC
  ACCAAGACCAGCCTCAGAAGTGCGGCCCCAACAGCCACTGTGAGATGGACTGCGAGGTTAACAATGAGGA
  CTTACTCTGTGTCCTCATTGATGATGGAGGATTCCTGGTGCTGTCAAACCAGAACCATCAGTGGGACCAG
  GTGGGCAGGTTCTTCAGTGAGGTGGATGCCAACCTGATGCTGGCACTCTACAATAACTCCTTCTACACCC
  GCAAGGAGTCCTATGACTATCAGGCAGCCTGTGCCCCTCAGCCCCCTGGCAACCTGGGTGCTGCACCCCG
  GGGTGTCTTTGTGCCCACCGTTGCAGATTTCCTTAACCTGGCCTGGTGGACCTCTGCTGCCGCCTGGTCC
  CTGTTCCAGCAGCTTCTCTACGGCCTCATCTACCACAGCTGGTTCCAAGCAGACCCCGCGGAGGCCGAGG
  GGAGCCCCGAGACGCGCGAGAGCAGCTGCGTCATGAAACAGACCCAGTACTACTTCGGCTCGGTAAACGC
  CTCCTACAACGCCATCATCGACTGCGGAAACTGCTCCAGGCTGTTCCACGCGCAGAGACTGACCAACACC
  AATCTTCTCTTTGTGGTGGCCGAGAAGCCGCTGTGCAGCCAGTGCGAGGCTGGCCGGCTGCTGCAGAAGG
  AGACGCACTCGGACGGCCCGGAGCAGTGTGAGCTAGTGCAGAGACCGCGATACCGGAGAGGCCCGCACAT
  CTGCTTCGACTACAACGCGACAGAAGATACCTCAGACTGTGGCCGCGGGGCCTCCTTCCCGCCGTCGCTG
  GGCGTCCTGGTCTCCCTGCAACTGCTGCTCCTCCTGGGCCTGCCGCCCCGGCCGCAGCCTCAAGTCCTCG
  TCCACGCCTCTCGCCGCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230662 representing NM_001174051
  Red=Cloning site Green=Tags(s)
  
  MAVPARTCGASRPGPARTARPWPGCGPHPGPGTRRPTSGPPRPLWLLLPLLPLLAAPGASAYSFPQQHTM
  QHWARRLEQEVDGVMRIFGGVQQLREIYKDNRNLFEVQENEPQKLVEKVAGDIESLLDRKVQALKRLADA
  AENFQKAHRWQDNIKEEDIVYYDAKADAELDDPESEDVERGSKASTLRLDFIEDPNFKNKVNYSYAAVQI
  PTDIYKGSTVILNELNWTEALENVFMENRRQDPTLLWQVFGSATGVTRYYPATPWRAPKKIDLYDVRRRP
  WYIQGASSPKDMVIIVDVSGSVSGLTLKLMKTSVCEMLDTLSDDDYVNVASFNEKAQPVSCFTHLVQANV
  RNKKVFKEAVQGMVAKGTTGYKAGFEYAFDQLQNSNITRANCNKMIMMFTDGGEDRVQDVFEKYNWPNRT
  VRVFTFSVGQHNYDVTPLQWMACANKGYYFEIPSIGAIRINTQEYLDVLGRPMVLAGKEAKQVQWTNVYE
  DALGLGLVVTGTLPVFNLTQDGPGEKKNQLILGVMGIDVALNDIKRLTPNYTLGANGYVFAIDLNGYVLL
  HPNLKPQTTNFREPVTLDFLDAELEDENKEEIRRSMIDGNKGHKQIRTLVKSLDERYIDEVTRNYTWVPI
  RSTNYSLGLVLPPYSTFYLQANLSDQILQVKLPISKLKDFEFLLPSSFESEGHVFIAPREYCKDLNASDN
  NTEFLKNFIELMEKVTPDSKQCNNFLLHNLILDTGITQQLVERVWRDQDLNTYSLLAVFAATDGGITRVF
  PNKAAEDWTENPEPFNASFYRRSLDNHGYVFKPPHQDALLRPLELENDTVGILVSTAVELSLGRRTLRPA
  VVGVKLDLEAWAEKFKVLASNRTHQDQPQKCGPNSHCEMDCEVNNEDLLCVLIDDGGFLVLSNQNHQWDQ
  VGRFFSEVDANLMLALYNNSFYTRKESYDYQAACAPQPPGNLGAAPRGVFVPTVADFLNLAWWTSAAAWS
  LFQQLLYGLIYHSWFQADPAEAEGSPETRESSCVMKQTQYYFGSVNASYNAIIDCGNCSRLFHAQRLTNT
  NLLFVVAEKPLCSQCEAGRLLQKETHSDGPEQCELVQRPRYRRGPHICFDYNATEDTSDCGRGASFPPSL
  GVLVSLQLLLLLGLPPRPQPQVLVHASRRL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001174051
• ORF Size: 3450 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001174051.3
• RefSeq ORF: 3453 bp
• Locus ID: 9254
• UniProt ID: Q9NY47
• Cytogenetics: 3p21.31
• Protein Families: Druggable Genome, Ion Channels: Other
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway
• MW: 130.3 kDa
• Gene Summary: Calcium channels mediate the entry of calcium ions into the cell upon membrane polarization. This gene encodes the alpha-2/delta subunit of the voltage-dependent calcium channel complex. The complex consists of the main channel-forming subunit alpha-1, and auxiliary subunits alpha-2/delta, beta, and gamma. The auxiliary subunits function in the assembly and membrane localization of the complex, and modulate calcium currents and channel activation/inactivation kinetics. The subunit encoded by this gene undergoes post-translational cleavage to yield the extracellular alpha2 peptide and a membrane-anchored delta polypeptide. This subunit is a receptor for the antiepileptic drug, gabapentin. Mutations in this gene are associated with early infantile epileptic encephalopathy. Single nucleotide polymorphisms in this gene are correlated with increased sensitivity to opioid drugs. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014]