GPRASP2 (NM_001184874) Human Tagged ORF Clone

CAT#: RC230528

GPRASP2 (Myc-DDK-tagged)-Human G protein-coupled receptor associated sorting protein 2 (GPRASP2), transcript variant 3

Product Images

Circular map for RC230528

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPRASP2
• Synonyms: DFNX7; GASP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230528 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGGGGCAGAGATTGAGCCTAGTGCCCAGGCCAAGCCTGAAAAGAAGGCTGGGGAAGAGGTTATCG
  CTGGGCCTGAGAGAGAGAATGATGTCCCTCTGGTGGTCAGACCCAAGGTTAGGACCCAGGCAACTACTGG
  GGCAAGGCCCAAAACTGAGACCAAGTCTGTGCCTGCGGCAAGGCCCAAAACTGAGGCCCAAGCAATGTCT
  GGGGCAAGGCCCAAAACTGAGGTCCAAGTAATGGGTGGTGCAAGACCCAAAACGGAGGCTCAAGGAATCA
  CAGGGGCCAGGCCCAAAACCGATGCCAGGGCAGTAGGTGGCGCTCGTTCTAAAACTGATGCCAAGGCAAT
  CCCTGGAGCAAGGCCCAAGGATGAGGCCCAGGCATGGGCCCAGAGTGAATTTGGGACTGAAGCAGTGTCA
  CAGGCAGAAGGAGTGTCCCAGACTAATGCCGTTGCTTGGCCACTGGCCACTGCTGAGTCTGGATCAGTTA
  CTAAATCTAAGGGCCTGTCTATGGATAGAGAACTAGTCAATGTGGATGCTGAAACCTTTCCTGGCACCCA
  GGGTCAGAAAGGAATCCAGCCCTGGTTTGGACCAGGGGAGGAGACTAATATGGGGTCTTGGTGCTATTCC
  AGGCCCAGGGCCAGAGAGGAGGCCTCTAATGAGTCTGGGTTCTGGTCAGCAGATGAGACCTCTACAGCGT
  CTTCTTTCTGGACTGGAGAAGAGACAAGTGTCAGATCATGGCCCAGGGAAGAGTCCAATACCAGGTCCAG
  GCACAGGGCTAAACATCAGACTAATCCCAGGTCCAGGCCCAGATCCAAGCAAGAAGCCTATGTTGATTCC
  TGGTCTGGATCTGAGGATGAGGCCAGCAACCCATTCTCCTTCTGGGTTGGAGAAAATACCAATAACTTGT
  TCAGGCCCAGAGTCAGGGAGGAGGCAAATATCAGGTCCAAGCTCAGGACAAATAGAGAAGATTGTTTTGA
  ATCTGAGTCTGAAGATGAGTTCTATAAGCAGTCCTGGGTTTTGCCTGGAGAAGAGGCCAATAGTAGATTC
  AGGCACAGAGACAAAGAAGATCCTAATACTGCCTTGAAACTCAGGGCCCAGAAAGATGTTGACAGTGATA
  GGGTCAAACAAGAACCCAGGTTTGAGGAGGAAGTCATTATTGGGTCCTGGTTCTGGGCAGAAAAAGAGGC
  CAGTTTGGAGGGTGGAGCTTCAGCAATCTGTGAATCTGAGCCAGGAACTGAGGAGGGGGCCATTGGCGGA
  TCCGCGTACTGGGCTGAGGAAAAGTCCAGTTTGGGGGCTGTGGCCAGAGAAGAGGCCAAGCCGGAGTCTG
  AAGAAGAGGCCATATTTGGGTCCTGGTTCTGGGACAGAGATGAGGCCTGCTTTGACCTAAATCCCTGTCC
  TGTGTACAAGGTCAGTGATAGGTTCAGAGATGCAGCTGAGGAGCTTAATGCATCCTCCAGGCCCCAAACC
  TGGGACGAGGTCACTGTTGAATTCAAACCTGGTCTTTTTCATGGGGTTGGCTTCCGATCCACAAGCCCCT
  TTGGAATTCCCGAAGAGGCTTCTGAAATGCTTGAGGCAAAGCCCAAGAACCTGGAACTTAGCCCAGAAGG
  AGAAGAGCAGGAATCTTTGCTTCAGCCTGATCAGCCTAGTCCTGAGTTCACATTTCAGTATGATCCTTCC
  TACCGGTCAGTCCGGGAAATTCGAGAGCATCTTAGGGCCAGGGAGAGTGCAGAGTCTGAGAGTTGGTCAT
  GCAGCTGCATACAATGTGAGCTGAAAATTGGTTCTGAAGAGTTTGAAGAATTCCTTTTATTAATGGACAA
  AATTCGGGATCCTTTTATTCATGAAATATCTAAAATTGCAATGGGTATGAGAAGTGCTTCTCAATTTACC
  CGAGATTTCATTCGAGATTCAGGTGTTGTCTCACTTATTGAAACCTTGCTTAATTATCCATCCTCTAGAG
  TTAGGACAAGTTTTTTGGAAAATATGATTCACATGGCTCCACCTTATCCAAATCTAAACATGATTGAGAC
  ATTCATATGTCAAGTGTGTGAGGAAACCCTTGCACATAGTGTGGATTCCCTTGAGCAGCTGACTGGAATA
  AGGATGCTTAGACACCTCACTATGACTATTGACTATCACACACTGATTGCCAACTATATGTCCGGGTTTC
  TCTCCTTATTAACCACAGCCAATGCGAGAACGAAGTTTCACGTTCTGAAAATGCTATTGAATTTGTCTGA
  AAATCCTGCTGTGGCAAAAAAACTATTCAGTGCCAAAGCTCTTTCAATATTTGTGGGTCTCTTTAACATA
  GAAGAGACAAATGATAATATTCAAATTGTTATTAAAATGTTTCAGAATATCAGTAACATTATAAAAAGTG
  GAAAGATGTCCTTAATTGATGATGATTTCAGTCTTGAGCCGCTTATTTCTGCATTTCGTGAATTTGAGGA
  GTTAGCTAAGCAACTACAAGCCCAAATAGACAACCAAAATGATCCTGAGGTGGGACAACAAAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230528 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTGAEIEPSAQAKPEKKAGEEVIAGPERENDVPLVVRPKVRTQATTGARPKTETKSVPAARPKTEAQAMS
  GARPKTEVQVMGGARPKTEAQGITGARPKTDARAVGGARSKTDAKAIPGARPKDEAQAWAQSEFGTEAVS
  QAEGVSQTNAVAWPLATAESGSVTKSKGLSMDRELVNVDAETFPGTQGQKGIQPWFGPGEETNMGSWCYS
  RPRAREEASNESGFWSADETSTASSFWTGEETSVRSWPREESNTRSRHRAKHQTNPRSRPRSKQEAYVDS
  WSGSEDEASNPFSFWVGENTNNLFRPRVREEANIRSKLRTNREDCFESESEDEFYKQSWVLPGEEANSRF
  RHRDKEDPNTALKLRAQKDVDSDRVKQEPRFEEEVIIGSWFWAEKEASLEGGASAICESEPGTEEGAIGG
  SAYWAEEKSSLGAVAREEAKPESEEEAIFGSWFWDRDEACFDLNPCPVYKVSDRFRDAAEELNASSRPQT
  WDEVTVEFKPGLFHGVGFRSTSPFGIPEEASEMLEAKPKNLELSPEGEEQESLLQPDQPSPEFTFQYDPS
  YRSVREIREHLRARESAESESWSCSCIQCELKIGSEEFEEFLLLMDKIRDPFIHEISKIAMGMRSASQFT
  RDFIRDSGVVSLIETLLNYPSSRVRTSFLENMIHMAPPYPNLNMIETFICQVCEETLAHSVDSLEQLTGI
  RMLRHLTMTIDYHTLIANYMSGFLSLLTTANARTKFHVLKMLLNLSENPAVAKKLFSAKALSIFVGLFNI
  EETNDNIQIVIKMFQNISNIIKSGKMSLIDDDFSLEPLISAFREFEELAKQLQAQIDNQNDPEVGQQS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001184874
• ORF Size: 2514 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001184874.2, NP_001171803.1
• RefSeq Size: 3946 bp
• RefSeq ORF: 2517 bp
• Locus ID: 114928
• UniProt ID: Q96D09
• Cytogenetics: Xq22.1
• Protein Families: Druggable Genome
• MW: 93.8 kDa
• Gene Summary: The protein encoded by this gene is a member of a family that regulates the activity of G protein-coupled receptors (GPCRs). The encoded protein has been shown to be capable of interacting with several GPCRs, including the M1 muscarinic acetylcholine receptor and the calcitonin receptor. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, May 2010]