GRID2IP (NM_001145118) Human Tagged ORF Clone

CAT#: RC227339

GRID2IP (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, delta 2 (Grid2) interacting protein (GRID2IP)

Product Images

Circular map for RC227339

• 

  Western blot validation of overexpression lysate (Cat# LY428702) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227339 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified GRID2IP protein (Cat# TP327339). The protein was produced from HEK293T cells transfected with GRID2IP cDNA clone (Cat# RC227339) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GRID2IP
• Synonyms: DELPHILIN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227339 representing NM_001145118
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCACTGCCACGCCGGCCACGAACCAGGGCTGGCCAGAGGACTTTGGCTTCCGGCTAGGTGGCT
  CTGGCCCCTGCTTCGTCCTGGAGGTGGCCAAGGGGAGCAGCGCGCATGCCGGAGGACTGCGGCCAGGAGA
  CCAGATCCTGGAGGTGGAGGGGCTGGCGGTGGGCGGTCTGAGCCGCGAGCGCCTCGTGCGCCTGGCACGG
  CGCTGCCCACGTGTGCCGCCCAGTCTGGGCGTGCTCCCGGCTCCCGACGGTGGCCCCGGCCCAGGATCCG
  GCCCCGCGGCCCCGACCACAGTCTTGCGGGCCCCGCGGTGCGGCCGCGGCCTAGCTCTGGGCCGTGAGCT
  GCTTCGCCTGGCCGGCCGCAAGCGCCCGGACGCGGTGCACCGAGAGCGCAGGCGCAAGGCCCAAGAGTTC
  AGCCGCAAGGTGGATGAAATCTTGGGGGACCAGCCAACTGCCAAGGAGCAGGTGTTCGCTGCACTGAAGC
  AGTTTGCAGCTGAGCAGCGGGTGGATGATCTGGTGTGGACTCTCACCCTGGCGCTGCCCCGAGAGGCCTG
  CGGGCCACTCCTGGACAACCTCAGGATCTTCATCCCCAAGAAGCACCGGGCGCGCTTCGACGAGGTGGTG
  TCTCAGGGCCTCCTGGGCAAGCTGTGCCGCGCACGCCGGGCCCAGGGCGCGCAGCGACTGCGCCGGAGCC
  GCAGCGAGGAGCGGCCGGAGCGCCTCCTGGTGTCCACGCGCGCCAGCGCCCCGCCGCGCCGCCCCGATGA
  GCCGCCCCCGCGCAGGGCCTCCTTGCTGGTGGGCGGCCTCGCCGGCCCCGGGGGCGCGCGCAGGACTGTC
  CGAGTCTACAAAGGCAACAAGAGCTTCGGCTTCACACTTCGCGGCCACGGGCCTGTCTGGATCGAGTCTG
  TCCTGCCTGGGAGCCCAGCTGACAATGCTGCCCTCAAGTCAGGTGACCGGATCCTCTTCCTCAATGGACT
  GGACATGAGGAACTGCTCCCACGACAAGGTGGTGTCCATGCTGCAGGGCAGTGGTGCCATGCCCACGCTG
  GTGGTGGAGGAAGGGCTCGTCCCATTTGCCAGTGACTCCGATTCTCTGGACTCACCCAACCCGTCGTCGG
  CGCTCACCTCCCTGCAGTGGGTGGCGGAGATCCTGCCGTCCAGCATCCGGGTCCAAGGGAGGACCTTCAG
  CCAGCAGCTGGAGCACCTACTCACACCTCCTGAGCGCTATGGGGTCTGCCGGGCCCTCGAGAGCTTCTTC
  CAGCACAGGAACATCGACACCCTCATCGTTGACGTCTACCCTGTGCTGGACACCCCTGCCAAGCAGGTCC
  TGTGGCAGTTCATCTACCAGCTGCTGACCTATGAGGAGCAGGAGCTCTGTCAGGAGAAAATCGCATGCTT
  CCTGGGCTACACGGCCATGACAGCAGAGCCGGAGCCTGAGCTGGACCTGGAGTCCGAGCCCACGCCTGAG
  CCCCAGCCGCGGAGCTCCCTGCGGGCTTCCTCCATGTGCCGCCGCAGCCTCCGGTCCCAGGGCCTGGAGG
  CCGGCCTCAGCTGCGGTCCCAGCGAGTGCCCTGAGATGCCTCTTCCCCTGATCCCAGGCGAGCGCCAGGC
  AGGCGACGGCACGTCCCTCCCTGAGACCCCCAACCCCAAGATGATGTCAGCCGTCTATGCAGAGCTTGAG
  TCTCGACTGAACAGCAGCTTCAAAGGGAAGATGGGGACCGTGTCCAAATCCCGGGCTTCCCCTCCAGGCC
  CCAGCCCAGCAGTCACCACAGGGCCCAGGACCCTGTCCGGCGTCTCATGGCCCAGCGAGCGACTCTTGCC
  CTCCCCCTGCTACCACCCGCTGTGTTCGGGGGGTCTGGCCTCCCCCAGCAGCTCTGAGTCCCACCCCTAC
  GCCAGCCTGGACAGCAGCAGGGCACCCTCCCCACAGCCAGGCCCCGGGCCCATCTGCCCCGACAGCCCCC
  CAAGCCCGGACCCCACCCGCCCGCCCAGCCGCAGGAAGCTCTTCACCTTCTCCCACCCTGTGCGAAGCCG
  CGATACTGACCGCTTCCTGGACGTGCTGAGCGAGCAGCTGGGCCCCCGGGTCACCATCGTGGATGATTTC
  CTGACCCCGGAGAACGACTACGAGGAGATGAGCTTCCATGATGACCAGGGCAGCTTCGTAACCAATGAGC
  GGAGCAGCGCCAGCGACTGCATCAGCAGCAGTGAAGAAGGCAGCTCCCTGACCTACTCCTCCATCTCTGA
  CCACATCCCCCCACCCCCGCTCAGCCCCCCACCACCGCCACCCCTGCCTTTCCATGACGCTAAGCCCAGC
  TCCCGCAGCTCTGATGGTTCCCGGGGCCCTGCTCAGGCGCTGGCCAAGCCCCTCACCCAACTCAGCCACC
  CAGTCCCTCCACCACCCCCACCGCCCCTGCCCCCACCCGTGCCCTGTGCACCCCCCATGCTGTCCCGGGG
  CCTGGGCCACCGGCGCAGTGAGACCAGCCACATGAGCGTCAAGCGCTTGCGGTGGGAACAGGTGGAGAAC
  TCAGAAGGCACCATCTGGGGTCAGCTCGGGGAAGACTCTGACTACGATAAGCTGAGTGACATGGTGAAAT
  ACCTCGACCTGGAGCTCCACTTCGGCACCCAGAAACCTGCTAAACCGGTGCCGGGGCCGGAGCCCTTCCG
  GAAGAAGGAGGTGGTGGAGATCCTGTCCCATAAGAAGGCCTACAACACCTCCATCCTCTTGGCACACCTG
  AAGCTGAGCCCCGCGGAGCTGCGCCAGGTGCTGATGAGCATGGAGCCCCGGCGCCTGGAGCCCGCACATC
  TCGCGCAGCTGCTGCTCTTCGCGCCCGACGCCGACGAGGAGCAGCGCTACCAGGCCTTCCGCGAGGCGCC
  CGGCCGCCTCAGCGAGCCGGACCAGTTCGTCCTGCAGATGCTGTCAGTTCCCGAATACAAGACACGCCTG
  CGCAGCCTCCACTTCCAGGCCACCCTCCAGGAGAAGACAGAGGAGATCCGAGGCAGCCTTGAATGCTTGC
  GCCAGGCCTCCCTGGAGCTCAAAAACAGTCGGAAGCTCGCCAAGATCCTGGAGTTTGTGTTGGCCATGGG
  CAACTATCTCAACGATGGACAGCCCAAAACCAACAAGACTACGGGCTTCAAGATCAACTTTCTGACAGAG
  CTGAACTCCACCAAGACAGTGGATGGGAAGTCCACCTTCCTGCACATCCTTGCCAAATCGCTGAGCCAGC
  ACTTCCCTGAACTCCTGGGCTTTGCTCAGGACCTGCCCACCGTGCCCCTGGCTGCCAAAGTGAACCAACG
  GGCCCTGACCAGTGACCTGGCTGACCTCCATGGCACTATCAGCGAGATACAGGATGCCTGCCAGAGCATT
  TCCCCCTCTAGCGAGGACAAGTTTGCAATGGTCATGTCGTCCTTCCTGGAGACGGCCCAGCCAGCACTTC
  GGGCGCTCGACGGGCTGCAGCGCGAGGCCATGGAGGAGCTGGGCAAGGCGCTGGCCTTCTTTGGGGAGGA
  TTCCAAGGCCACCACCTCTGAGGCTTTCTTCGGCATCTTTGCAGAGTTCATGAGCAAATTCGAGCGAGCG
  CTGAGTGACCTGCAGGCCGGGGAGGGCCTGCGCAGCTCCGGGATGGTTTCACCCCTGGCCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227339 representing NM_001145118
  Red=Cloning site Green=Tags(s)
  
  MATTATPATNQGWPEDFGFRLGGSGPCFVLEVAKGSSAHAGGLRPGDQILEVEGLAVGGLSRERLVRLAR
  RCPRVPPSLGVLPAPDGGPGPGSGPAAPTTVLRAPRCGRGLALGRELLRLAGRKRPDAVHRERRRKAQEF
  SRKVDEILGDQPTAKEQVFAALKQFAAEQRVDDLVWTLTLALPREACGPLLDNLRIFIPKKHRARFDEVV
  SQGLLGKLCRARRAQGAQRLRRSRSEERPERLLVSTRASAPPRRPDEPPPRRASLLVGGLAGPGGARRTV
  RVYKGNKSFGFTLRGHGPVWIESVLPGSPADNAALKSGDRILFLNGLDMRNCSHDKVVSMLQGSGAMPTL
  VVEEGLVPFASDSDSLDSPNPSSALTSLQWVAEILPSSIRVQGRTFSQQLEHLLTPPERYGVCRALESFF
  QHRNIDTLIVDVYPVLDTPAKQVLWQFIYQLLTYEEQELCQEKIACFLGYTAMTAEPEPELDLESEPTPE
  PQPRSSLRASSMCRRSLRSQGLEAGLSCGPSECPEMPLPLIPGERQAGDGTSLPETPNPKMMSAVYAELE
  SRLNSSFKGKMGTVSKSRASPPGPSPAVTTGPRTLSGVSWPSERLLPSPCYHPLCSGGLASPSSSESHPY
  ASLDSSRAPSPQPGPGPICPDSPPSPDPTRPPSRRKLFTFSHPVRSRDTDRFLDVLSEQLGPRVTIVDDF
  LTPENDYEEMSFHDDQGSFVTNERSSASDCISSSEEGSSLTYSSISDHIPPPPLSPPPPPPLPFHDAKPS
  SRSSDGSRGPAQALAKPLTQLSHPVPPPPPPPLPPPVPCAPPMLSRGLGHRRSETSHMSVKRLRWEQVEN
  SEGTIWGQLGEDSDYDKLSDMVKYLDLELHFGTQKPAKPVPGPEPFRKKEVVEILSHKKAYNTSILLAHL
  KLSPAELRQVLMSMEPRRLEPAHLAQLLLFAPDADEEQRYQAFREAPGRLSEPDQFVLQMLSVPEYKTRL
  RSLHFQATLQEKTEEIRGSLECLRQASLELKNSRKLAKILEFVLAMGNYLNDGQPKTNKTTGFKINFLTE
  LNSTKTVDGKSTFLHILAKSLSQHFPELLGFAQDLPTVPLAAKVNQRALTSDLADLHGTISEIQDACQSI
  SPSSEDKFAMVMSSFLETAQPALRALDGLQREAMEELGKALAFFGEDSKATTSEAFFGIFAEFMSKFERA
  LSDLQAGEGLRSSGMVSPLAW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145118
• ORF Size: 3633 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145118.1, NP_001138590.1
• RefSeq ORF: 3636 bp
• Locus ID: 392862
• UniProt ID: A4D2P6
• Cytogenetics: 7p22.1
• MW: 132.1 kDa
• Gene Summary: Glutamate receptor delta-2 (GRID2; MIM 602368) is predominantly expressed at parallel fiber-Purkinje cell postsynapses and plays crucial roles in synaptogenesis and synaptic plasticity. GRID2IP1 interacts with GRID2 and may control GRID2 signaling in Purkinje cells (Matsuda et al., 2006 [PubMed 16835239]).[supplied by OMIM, Mar 2008]