CACNA1C (NM_001129844) Human Tagged ORF Clone

CAT#: RC226459

CACNA1C (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant 19

Product Images

Circular map for RC226459

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CACNA1C
• Synonyms: CACH2; CACN2; CACNL1A1; CaV1.2; CCHL1A1; LQT8; TS; TS. LQT8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226459 representing NM_001129844
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCAATGAGAATACGAGGATGTACATTCCAGAGGAAAACCACCAAGGTTCCAACTATGGGAGCCCAC
  GCCCCGCCCATGCCAACATGAATGCCAATGCGGCAGCGGGGCTGGCCCCTGAGCACATCCCCACCCCGGG
  GGCTGCCCTGTCGTGGCAGGCGGCCATCGACGCAGCCCGGCAGGCTAAGCTGATGGGCAGCGCTGGCAAT
  GCGACCATCTCCACAGTCAGCTCCACGCAGCGGAAGCGGCAGCAATATGGGAAACCCAAGAAGCAGGGCA
  GCACCACGGCCACACGCCCGCCCCGAGCCCTGCTCTGCCTGACCCTGAAGAACCCCATCCGGAGGGCCTG
  CATCAGCATTGTCGAATGGAAACCATTTGAAATAATTATTTTACTGACTATTTTTGCCAATTGTGTGGCC
  TTAGCGATCTATATTCCCTTTCCAGAAGATGATTCCAACGCCACCAATTCCAACCTGGAACGAGTGGAAT
  ATCTCTTTCTCATAATTTTTACGGTGGAAGCGTTTTTAAAAGTAATCGCCTATGGACTCCTCTTTCACCC
  CAATGCCTACCTCCGCAACGGCTGGAACCTACTAGATTTTATAATTGTGGTTGTGGGGCTTTTTAGTGCA
  ATTTTAGAACAAGCAACCAAAGCAGATGGGGCAAACGCTCTCGGAGGGAAAGGGGCCGGATTTGATGTGA
  AGGCGCTGAGGGCCTTCCGCGTGCTGCGCCCCCTGCGGCTGGTGTCCGGAGTCCCAAGTCTCCAGGTGGT
  CCTGAATTCCATCATCAAGGCCATGGTCCCCCTGCTGCACATCGCCCTGCTTGTGCTGTTTGTCATCATC
  ATCTACGCCATCATCGGCTTGGAGCTCTTCATGGGGAAGATGCACAAGACCTGCTACAACCAGGAGGGCA
  TAGCAGCAGAAGATGACCCTTCCCCTTGTGCGCTGGAAACGGGCCACGGGCGGCAGTGCCAGAACGGCAC
  GGTGTGCAAGCCCGGCTGGGATGGTCCCAAGCACGGCATCACCAACTTTGACAACTTTGCCTTCGCCATG
  CTCACGGTGTTCCAGTGCATCACCATGGAGGGCTGGACGGACGTGCTGTACTGGGTCAATGATGCCGTAG
  GAAGGGACTGGCCCTGGATCTATTTTGTTACACTAATCATCATAGGGTCATTTTTTGTACTTAACTTGGT
  TCTCGGTGTGCTTAGCGGAGAGTTTTCCAAAGAGAGGGAGAAGGCCAAGGCCCGGGGAGATTTCCAGAAG
  CTGCGGGAGAAGCAGCAGCTAGAAGAGGATCTCAAAGGCTACCTGGATTGGATCACTCAGGCCGAAGACA
  TCGATCCTGAGAATGAGGACGAAGGCATGGATGAGGAGAAGCCCCGAAACATGAGCATGCCCACCAGTGA
  GACCGAGTCCGTCAACACCGAAAACGTGGCTGGAGGTGACATCGAGGGAGAAAACTGCGGGGCCAGGCTG
  GCCCACCGGATCTCCAAGTCAAAGTTCAGCCGCTACTGGCGCCGGTGGAATCGGTTCTGCAGAAGGAAGT
  GCCGCGCCGCAGTCAAGTCTAATGTCTTCTACTGGCTGGTGATTTTCCTGGTGTTCCTCAACACGCTCAC
  CATTGCCTCTGAGCACTACAACCAGCCCAACTGGCTCACAGAAGTCCAAGACACGGCAAACAAGGCCCTG
  CTGGCCCTGTTCACGGCAGAGATGCTCCTGAAGATGTACAGCCTGGGCCTGCAGGCCTACTTCGTGTCCC
  TCTTCAACCGCTTTGACTGCTTCGTCGTGTGTGGCGGCATCCTGGAGACCATCCTGGTGGAGACCAAGAT
  CATGTCCCCACTGGGCATCTCCGTGCTCAGATGCGTCCGGCTGCTGAGGATTTTCAAGATCACGAGGTAC
  TGGAACTCCTTGAGCAACCTGGTGGCATCCTTGCTGAACTCTGTGCGCTCCATCGCCTCCCTGCTCCTTC
  TCCTCTTCCTCTTCATCATCATCTTCTCCCTCCTGGGGATGCAGCTCTTTGGAGGAAAGTTCAACTTTGA
  TGAGATGCAGACCCGGAGGAGCACATTCGATAACTTCCCCCAGTCCCTCCTCACTGTGTTTCAGATCCTG
  ACCGGGGAGGACTGGAATTCGGTGATGTATGATGGGATCATGGCTTATGGCGGCCCCTCTTTTCCAGGGA
  TGTTAGTCTGTATTTACTTCATCATCCTCTTCATCTGTGGAAACTATATCCTACTGAATGTGTTCTTGGC
  CATTGCTGTGGACAACCTGGCTGATGCTGAGAGCCTCACATCTGCCCAAAAGGAGGAGGAAGAGGAGAAG
  GAGAGAAAGAAGCTGGCCAGGACTGCCAGCCCAGAGAAGAAACAAGAGTTGGTGGAGAAGCCGGCAGTGG
  GGGAATCCAAGGAGGAGAAGATTGAGCTGAAATCCATCACGGCTGACGGAGAGTCTCCACCCGCCACCAA
  GATCAACATGGATGACCTCCAGCCCAATGAAAATGAGGATAAGAGCCCCTACCCCAACCCAGAAACTACA
  GGAGAAGAGGATGAGGAGGAGCCAGAGATGCCTGTCGGCCCTCGCCCACGACCACTCTCTGAGCTTCACC
  TTAAGGAAAAGGCAGTGCCCATGCCAGAAGCCAGCGCGTTTTTCATCTTCAGCTCTAACAACAGGTTTCG
  CCTCCAGTGCCACCGCATTGTCAATGACACGATCTTCACCAACCTGATCCTCTTCTTCATTCTGCTCAGC
  AGCATTTCCCTGGCTGCTGAGGACCCGGTCCAGCACACCTCCTTCAGGAACCATATTCTGTTTTATTTTG
  ATATTGTTTTTACCACCATTTTCACCATTGAAATTGCTCTGAAGATGACTGCTTATGGGGCTTTCTTGCA
  CAAGGGTTCTTTCTGCCGGAACTACTTCAACATCCTGGACCTGCTGGTGGTCAGCGTGTCCCTCATCTCC
  TTTGGCATCCAGTCCAGTGCAATCAATGTCGTGAAGATCTTGCGAGTCCTGCGAGTACTCAGGCCCCTGA
  GGGCCATCAACAGGGCCAAGGGGCTAAAGCATGTGGTTCAGTGTGTGTTTGTCGCCATCCGGACCATCGG
  GAACATCGTGATTGTCACCACCCTGCTGCAGTTCATGTTTGCCTGCATCGGGGTCCAGCTCTTCAAGGGA
  AAGCTGTACACCTGTTCAGACAGTTCCAAGCAGACAGAGGCGGAATGCAAGGGCAACTACATCACGTACA
  AAGACGGGGAGGTTGACCACCCCATCATCCAACCCCGCAGCTGGGAGAACAGCAAGTTTGACTTTGACAA
  TGTTCTGGCAGCCATGATGGCCCTCTTCACCGTCTCCACCTTCGAAGGGTGGCCAGAGCTGCTGTACCGC
  TCCATCGACTCCCACACGGAAGACAAGGGCCCCATCTACAACTACCGTGTGGAGATCTCCATCTTCTTCA
  TCATCTACATCATCATCATCGCCTTCTTCATGATGAACATCTTCGTGGGCTTCGTCATCGTCACCTTTCA
  GGAGCAGGGGGAGCAGGAGTACAAGAACTGTGAGCTGGACAAGAACCAGCGACAGTGCGTGGAATACGCC
  CTCAAGGCCCGGCCCCTGCGGAGGTACATCCCCAAGAACCAGCACCAGTACAAAGTGTGGTACGTGGTCA
  ACTCCACCTACTTCGAGTACCTGATGTTCGTCCTCATCCTGCTCAACACCATCTGCCTGGCCATGCAGCA
  CTACGGCCAGAGCTGCCTGTTCAAAATCGCCATGAACATCCTCAACATGCTCTTCACTGGCCTCTTCACC
  GTGGAGATGATCCTGAAGCTCATTGCCTTCAAACCCAAGGGTTACTTTAGTGATCCCTGGAATGTTTTTG
  ACTTCCTCATCGTAATTGGCAGCATAATTGACGTCATTCTCAGTGAGACTAATCCAGCTGAACATACCCA
  ATGCTCTCCCTCTATGAACGCAGAGGAAAACTCCCGCATCTCCATCACCTTCTTCCGCCTGTTCCGGGTC
  ATGCGTCTGGTGAAGCTGCTGAGCCGTGGGGAGGGCATCCGGACGCTGCTGTGGACCTTCATCAAGTCCT
  TCCAGGCCCTGCCCTATGTGGCCCTCCTGATCGTGATGCTGTTCTTCATCTACGCGGTGATCGGGATGCA
  GGTGTTTGGGAAAATTGCCCTGAATGATACCACAGAGATCAACCGGAACAACAACTTTCAGACCTTCCCC
  CAGGCCGTGCTGCTCCTCTTCAGGTGTGCCACCGGGGAGGCCTGGCAGGACATCATGCTGGCCTGCATGC
  CAGGCAAGAAGTGTGCCCCAGAGTCCGAGCCCAGCAACAGCACGGAGGGTGAAACACCCTGTGGTAGCAG
  CTTTGCTGTCTTCTACTTCATCAGCTTCTACATGCTCTGTGCCTTCCTGATCATCAACCTCTTTGTAGCT
  GTCATCATGGACAACTTTGACTACCTGACAAGGGACTGGTCCATCCTTGGTCCCCACCACCTGGATGAGT
  TTAAAAGAATCTGGGCAGAGTATGACCCTGAAGCCAAGGGTCGTATCAAACACCTGGATGTGGTGACCCT
  CCTCCGGCGGATTCAGCCGCCACTAGGTTTTGGGAAGCTGTGCCCTCACCGCGTGGCTTGCAAACGCCTG
  GTCTCCATGAACATGCCTCTGAACAGCGACGGGACAGTCATGTTCAATGCCACCCTGTTTGCCCTGGTCA
  GGACGGCCCTGAGGATCAAAACAGAAGGGAACCTAGAACAAGCCAATGAGGAGCTGCGGGCGATCATCAA
  GAAGATCTGGAAGCGGACCAGCATGAAGCTGCTGGACCAGGTGGTGCCCCCTGCAGGTGATGATGAGGTC
  ACCGTTGGCAAGTTCTACGCCACGTTCCTGATCCAGGAGTACTTCCGGAAGTTCAAGAAGCGCAAAGAGC
  AGGGCCTTGTGGGCAAGCCCTCCCAGAGGAACGCGCTGTCTCTGCAGGCTGGCTTGCGCACACTGCATGA
  CATCGGGCCTGAGATCCGACGGGCCATCTCTGGAGATCTCACCGCTGAGGAGGAGCTGGACAAGGCCATG
  AAGGAGGCTGTGTCCGCTGCTTCTGAAGATGACATCTTCAGGAGGGCCGGTGGCCTGTTCGGCAACCACG
  TCAGCTACTACCAAAGCGACGGCCGGAGCGCCTTCCCCCAGACCTTCACCACTCAGCGCCCGCTGCACAT
  CAACAAGGCGGGCAGCAGCCAGGGCGACACTGAGTCGCCATCCCACGAGAAGCTGGTGGACTCCACCTTC
  ACCCCGAGCAGCTACTCGTCCACCGGCTCCAACGCCAACATCAACAACGCCAACAACACCGCCCTGGGTC
  GCCTCCCTCGCCCCGCCGGCTACCCCAGCACGGTCAGCACTGTGGAGGGCCACGGGCCCCCCTTGTCCCC
  TGCCATCCGGGTGCAGGAGGTGGCGTGGAAGCTCAGCTCCAACAGGTGCCACTCCCGGGAGAGCCAGGCA
  GCCATGGCGGGTCAGGAGGAGACGTCTCAGGATGAGACCTATGAAGTGAAGATGAACCATGACACGGAGG
  CCTGCAGTGAGCCCAGCCTGCTCTCCACAGAGATGCTCTCCTACCAGGATGACGAAAATCGGCAACTGAC
  GCTCCCAGAGGAGGACAAGAGGGACATCCGGCAATCTCCGAAGAGGGGTTTCCTCCGCTCTGCCTCACTA
  GGTCGAAGGGCCTCCTTCCACCTGGAATGTCTGAAGCGACAGAAGGACCGAGGGGGAGACATCTCTCAGA
  AGACAGTCCTGCCCTTGCATCTGGTTCATCATCAGGCATTGGCAGTGGCAGGCCTGAGCCCCCTCCTCCA
  GAGAAGCCATTCCCCTGCCTCATTCCCTAGGCCTTTTGCCACCCCACCAGCCACACCTGGCAGCCGAGGC
  TGGCCCCCACAGCCCGTCCCCACCCTGCGGCTTGAGGGGGTCGAGTCCAGTGAGAAACTCAACAGCAGCT
  TCCCATCCATCCACTGCGGCTCCTGGGCTGAGACCACCCCCGGTGGCGGGGGCAGCAGCGCCGCCCGGAG
  AGTCCGGCCCGTCTCCCTCATGGTGCCCAGCCAGGCTGGGGCCCCAGGGAGGCAGTTCCACGGCAGTGCC
  AGCAGCCTGGTGGAAGCGGTCTTGATTTCAGAAGGACTGGGGCAGTTTGCTCAAGATCCCAAGTTCATCG
  AGGTCACCACCCAGGAGCTGGCCGACGCCTGCGACATGACCATAGAGGAGATGGAGAGCGCGGCCGACAA
  CATCCTCAGCGGGGGCGCCCCACAGAGCCCCAATGGCGCCCTCTTACCCTTTGTGAACTGCAGGGACGCG
  GGGCAGGACCGAGCCGGGGGCGAAGAGGACGCGGGCTGTGTGCGCGCGCGGGGTCGACCGAGTGAGGAGG
  AGCTCCAGGACAGCAGGGTCTACGTCAGCAGCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226459 representing NM_001129844
  Red=Cloning site Green=Tags(s)
  
  MVNENTRMYIPEENHQGSNYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGN
  ATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVA
  LAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSA
  ILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVII
  IYAIIGLELFMGKMHKTCYNQEGIAAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAM
  LTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQK
  LREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEEKPRNMSMPTSETESVNTENVAGGDIEGENCGARL
  AHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPNWLTEVQDTANKAL
  LALFTAEMLLKMYSLGLQAYFVSLFNRFDCFVVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRY
  WNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQIL
  TGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEK
  ERKKLARTASPEKKQELVEKPAVGESKEEKIELKSITADGESPPATKINMDDLQPNENEDKSPYPNPETT
  GEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSSNNRFRLQCHRIVNDTIFTNLILFFILLS
  SISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLIS
  FGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKG
  KLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYR
  SIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYA
  LKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFT
  VEMILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNPAEHTQCSPSMNAEENSRISITFFRLFRV
  MRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFP
  QAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPSNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVA
  VIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRL
  VSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEV
  TVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDIGPEIRRAISGDLTAEEELDKAM
  KEAVSAASEDDIFRRAGGLFGNHVSYYQSDGRSAFPQTFTTQRPLHINKAGSSQGDTESPSHEKLVDSTF
  TPSSYSSTGSNANINNANNTALGRLPRPAGYPSTVSTVEGHGPPLSPAIRVQEVAWKLSSNRCHSRESQA
  AMAGQEETSQDETYEVKMNHDTEACSEPSLLSTEMLSYQDDENRQLTLPEEDKRDIRQSPKRGFLRSASL
  GRRASFHLECLKRQKDRGGDISQKTVLPLHLVHHQALAVAGLSPLLQRSHSPASFPRPFATPPATPGSRG
  WPPQPVPTLRLEGVESSEKLNSSFPSIHCGSWAETTPGGGGSSAARRVRPVSLMVPSQAGAPGRQFHGSA
  SSLVEAVLISEGLGQFAQDPKFIEVTTQELADACDMTIEEMESAADNILSGGAPQSPNGALLPFVNCRDA
  GQDRAGGEEDAGCVRARGRPSEEELQDSRVYVSSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001129844
• ORF Size: 6405 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001129844.1, NP_001123316.1
• RefSeq ORF: 6408 bp
• Locus ID: 775
• UniProt ID: Q13936
• Cytogenetics: 12p13.33
• Protein Families: Druggable Genome, Ion Channels: Calcium, Transmembrane
• Protein Pathways: Alzheimer's disease, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Calcium signaling pathway, Cardiac muscle contraction, Dilated cardiomyopathy, GnRH signaling pathway, Hypertrophic cardiomyopathy (HCM), Long-term potentiation, MAPK signaling pathway, Type II diabetes mellitus, Vascular smooth muscle contraction
• MW: 239.2 kDa
• Gene Summary: This gene encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by this gene binds to and is inhibited by dihydropyridine. Alternative splicing results in many transcript variants encoding different proteins. Some of the predicted proteins may not produce functional ion channel subunits. [provided by RefSeq, Oct 2012]