CACNA1C Mouse Monoclonal Antibody [Clone ID: S57-46]

CAT#: TA309306

Mouse Monoclonal anti-CACNA1C Antibody

Product Images

IHC analysis of Cav1.2 in human hippocampus tissues.

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  Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.2 Monoclonal Antibody, Clone S57 (TA309306). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav1.2 Monoclonal Antibody (TA309306) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. (B) Anti-Cav1.2 Antibody. (C) Composite. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cav1.2 Antibody. (D) Composite.

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  Immunoprecipitation analysis using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57 (TA309306). Tissue: INS-1E cells. Species: Rat. Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody (TA309306) at 1:200. Courtesy of: Merrie Mosedale.

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  Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.2 Monoclonal Antibody, Clone S57 (TA309306). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav1.2 Monoclonal Antibody (TA309306) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav1.2 Antibody (D) Composite.

Specifications

Product Data

• Clone Name: S57-46
• Applications: ICC/IF, IF, IHC, IP
• Recommended Dilution: WB 1-10ug/ml; IHC/ICC 0.1-1.0ug/ml; IF 1.0-10ug/ml
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Fusion protein amino acids 1507-1733 (intracellular carboxyl terminus) of rabbit Cav1.2, accession number P15381
• Formulation: PBS pH7.4, 50% glycerol
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: calcium voltage-gated channel subunit alpha1 C
• Database Link:
  NP_000710
  Entrez Gene 12288 MouseEntrez Gene 24239 RatEntrez Gene 775 Human
  UniProt ID Q13936
• Synonyms: CACH2; CACN2; CACNL1A1; CaV1.2; CCHL1A1; LQT8; TS
• Note: Detects ~240kDa (varies with cell background due to glycosylation)

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Calcium, Transmembrane
• Protein Pathways: Alzheimer's disease, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Calcium signaling pathway, Cardiac muscle contraction, Dilated cardiomyopathy, GnRH signaling pathway, Hypertrophic cardiomyopathy (HCM), Long-term potentiation, MAPK signaling pathway, Type II diabetes mellitus, Vascular smooth muscle contraction