CASK (NM_001126055) Human Tagged ORF Clone

CAT#: RC226242

CASK (Myc-DDK-tagged)-Human calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK), transcript variant 3

Product Images

Circular map for RC226242

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CASK
• Synonyms: CAGH39; CAMGUK; CMG; FGS4; hCASK; LIN2; MICPCH; MRXSNA; TNRC8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226242 representing NM_001126055
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACGACGACGTGCTGTTCGAGGATGTGTACGAGCTGTGCGAGGTGATCGGAAAGGGTCCCTTCA
  GTGTTGTACGACGATGTATCAACAGAGAAACTGGGCAACAATTTGCTGTAAAAATTGTTGATGTAGCCAA
  GTTCACATCAAGTCCAGGGTTAAGTACAGAAGATCTAAAGCGGGAAGCCAGTATCTGTCATATGCTGAAA
  CATCCACACATTGTAGAGTTATTGGAGACATATAGCTCAGATGGAATGCTTTACATGGTTTTCGAATTTA
  TGGATGGAGCAGATCTGTGTTTTGAAATCGTAAAGCGAGCTGACGCTGGTTTTGTGTACAGTGAAGCTGT
  AGCCAGCCATTATATGAGACAGATACTGGAAGCTCTACGCTACTGCCATGATAATAACATAATTCACAGG
  GATGTGAAGCCCCACTGTGTTCTCCTTGCCTCAAAAGAAAACTCGGCACCTGTTAAACTTGGAGGCTTTG
  GGGTAGCTATTCAATTAGGGGAGTCTGGACTTGTAGCTGGAGGACGTGTTGGAACACCTCATTTTATGGC
  ACCAGAAGTGGTCAAAAGAGAGCCTTACGGAAAGCCTGTAGACGTCTGGGGGTGCGGTGTGATCCTTTTT
  ATCCTGCTCAGTGGTTGTTTGCCTTTTTACGGAACCAAGGAAAGATTGTTTGAAGGCATTATTAAAGGAA
  AATATAAGATGAATCCAAGGCAGTGGAGCCATATCTCTGAAAGTGCCAAAGACCTAGTACGTCGCATGCT
  GATGCTGGATCCAGCTGAAAGGATCACTGTTTATGAAGCACTGAATCACCCATGGCTTAAGGAGCGGGAT
  CGTTACGCCTACAAGATTCATCTTCCAGAAACAGTAGAGCAGCTGAGGAAATTCAATGCAAGGAGGAAAC
  TAAAGGGTGCAGTACTAGCCGCTGTGTCAAGTCACAAATTCAACTCATTCTATGGGGATCCCCCTGAAGA
  GTTACCAGATTTCTCCGAAGACCCTACCTCCTCAGGAGCAGTCTCACAGGTGCTGGACAGCCTGGAAGAG
  ATTCATGCGCTTACAGACTGCAGTGAAAAGGACCTAGATTTTCTACACAGTGTTTTCCAGGATCAGCATC
  TTCACACACTACTAGATCTGTATGACAAAATTAACACAAAGTCTTCACCACAAATCAGGAATCCTCCAAG
  CGATGCAGTACAGAGAGCCAAAGAGGTATTGGAAGAAATTTCATGTTACCCTGAGAATAACGACGCAAAG
  GAACTAAAGCGTATTTTAACACAACCTCATTTCATGGCCTTACTTCAGACTCACGACGTAGTGGCACATG
  AAGTTTACAGTGATGAAGCATTGAGGGTCACACCTCCTCCCACCTCTCCCTATTTAAACGGCGATTCTCC
  AGAAAGTGCTAACGGAGACATGGATATGGAGAATGTGACCAGAGTTCGGCTGGTACAGTTTCAAAAGAAC
  ACAGATGAACCAATGGGAATCACTTTAAAAATGAATGAACTAAATCATTGTATTGTTGCAAGAATTATGC
  ATGGGGGCATGATTCACAGGCAAGGTACACTTCATGTTGGTGATGAAATTCGAGAAATCAATGGCATCAG
  TGTGGCTAACCAAACAGTGGAACAACTGCAAAAAATGCTTAGGGAAATGCGGGGGAGTATTACCTTCAAG
  ATTGTGCCAAGTTACCGCACTCAGTCTTCGTCCTGTGAGGACTTGCCATCAACTACCCAACCAAAAGGAC
  GACAGATCTATGTAAGAGCACAATTTGAATATGATCCAGCCAAGGATGACCTCATCCCCTGTAAAGAAGC
  TGGCATTCGATTCAGAGTTGGTGACATCATCCAGATTATTAGTAAGGATGATCATAATTGGTGGCAGGGT
  AAACTGGAAAACTCCAAAAATGGAACTGCAGGTCTCATTCCTTCTCCTGAACTTCAGGAATGGCGAGTAG
  CTTGCATTGCCATGGAGAAGACCAAACAGGAGCAGCAGGCCAGCTGTACTTGGTTTGGCAAGAAAAAGAA
  GCAGTACAAAGATAAATATTTGGCAAAGCACAATGCAGTGTTTGATCAATTAGATCTTGTCACATATGAA
  GAAGTAGTAAAACTGCCAGCATTCAAGAGGAAAACACTAGTCTTATTAGGCGCACATGGTGTTGGGAGAA
  GACACATAAAAAACACTCTCATCACAAAGCACCCAGACCGGTTTGCGTACCCTATTCCACATACAACCAG
  ACCTCCAAAGAAAGACGAAGAAAATGGAAAGAATTATTACTTTGTATCTCATGACCAAATGATGCAAGAC
  ATCTCTAATAACGAGTACTTGGAGTACGGCAGCCACGAGGATGCGATGTATGGGACAAAACTGGAGACCA
  TCCGGAAGATCCACGAGCAGGGGCTGATTGCAATACTGGACGTGGAGCCTCAGGCACTGAAGGTCCTGAG
  AACTGCAGAGTTTGCTCCTTTTGTTGTTTTCATTGCTGCACCAACTATTACTCCAGGTTTAAATGAGGAT
  GAATCTCTTCAGCGTCTGCAGAAGGAGTCTGACATCTTACAGAGAACATATGCACACTACTTCGATCTCA
  CAATTATCAACAATGAAATTGATGAGACAATCAGACATCTGGAGGAAGCTGTTGAGCTCGTGTGCACAGC
  CCCACAGTGGGTCCCTGTCTCCTGGGTCTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226242 representing NM_001126055
  Red=Cloning site Green=Tags(s)
  
  MADDDVLFEDVYELCEVIGKGPFSVVRRCINRETGQQFAVKIVDVAKFTSSPGLSTEDLKREASICHMLK
  HPHIVELLETYSSDGMLYMVFEFMDGADLCFEIVKRADAGFVYSEAVASHYMRQILEALRYCHDNNIIHR
  DVKPHCVLLASKENSAPVKLGGFGVAIQLGESGLVAGGRVGTPHFMAPEVVKREPYGKPVDVWGCGVILF
  ILLSGCLPFYGTKERLFEGIIKGKYKMNPRQWSHISESAKDLVRRMLMLDPAERITVYEALNHPWLKERD
  RYAYKIHLPETVEQLRKFNARRKLKGAVLAAVSSHKFNSFYGDPPEELPDFSEDPTSSGAVSQVLDSLEE
  IHALTDCSEKDLDFLHSVFQDQHLHTLLDLYDKINTKSSPQIRNPPSDAVQRAKEVLEEISCYPENNDAK
  ELKRILTQPHFMALLQTHDVVAHEVYSDEALRVTPPPTSPYLNGDSPESANGDMDMENVTRVRLVQFQKN
  TDEPMGITLKMNELNHCIVARIMHGGMIHRQGTLHVGDEIREINGISVANQTVEQLQKMLREMRGSITFK
  IVPSYRTQSSSCEDLPSTTQPKGRQIYVRAQFEYDPAKDDLIPCKEAGIRFRVGDIIQIISKDDHNWWQG
  KLENSKNGTAGLIPSPELQEWRVACIAMEKTKQEQQASCTWFGKKKKQYKDKYLAKHNAVFDQLDLVTYE
  EVVKLPAFKRKTLVLLGAHGVGRRHIKNTLITKHPDRFAYPIPHTTRPPKKDEENGKNYYFVSHDQMMQD
  ISNNEYLEYGSHEDAMYGTKLETIRKIHEQGLIAILDVEPQALKVLRTAEFAPFVVFIAAPTITPGLNED
  ESLQRLQKESDILQRTYAHYFDLTIINNEIDETIRHLEEAVELVCTAPQWVPVSWVY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001126055
• ORF Size: 2691 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001126055.2, NP_001119527.1
• RefSeq ORF: 2694 bp
• Locus ID: 8573
• UniProt ID: O14936
• Cytogenetics: Xp11.4
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Tight junction
• MW: 101.9 kDa
• Gene Summary: This gene encodes a calcium/calmodulin-dependent serine protein kinase. The encoded protein is a MAGUK (membrane-associated guanylate kinase) protein family member. These proteins are scaffold proteins and the encoded protein is located at synapses in the brain. Mutations in this gene are associated with FG syndrome 4, intellectual disability and microcephaly with pontine and cerebellar hypoplasia, and a form of X-linked intellectual disability. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2017]