PER1 (NM_002616) Human Tagged ORF Clone

CAT#: RC224777

PER1 (Myc-DDK-tagged)-Human period homolog 1 (Drosophila) (PER1)

Product Images

Circular map for RC224777

• 

  Western blot validation of overexpression lysate (Cat# LY419216) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224777 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PER1
• Synonyms: hPER; PER; RIGUI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224777 representing NM_002616
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGCCCCCTAGAAGGGGCTGATGGGGGAGGGGACCCCAGGCCTGGGGAATCATTTTGTCCTGGGG
  GCGTCCCATCCCCTGGGCCCCCACAGCACCGGCCTTGCCCAGGCCCCAGCCTGGCCGATGACACCGATGC
  CAACAGCAATGGTTCAAGTGGCAATGAGTCCAACGGGCATGAGTCTAGAGGCGCATCTCAGCGGAGCTCA
  CACAGCTCCTCCTCAGGCAACGGCAAGGACTCAGCCCTGCTGGAGACCACTGAGAGCAGCAAGAGCACAA
  ACTCTCAGAGCCCATCCCCACCCAGCAGTTCCATTGCCTACAGCCTCCTGAGTGCCAGCTCAGAGCAGGA
  CAACCCGTCCACCAGTGGCTGCAGCAGTGAACAGTCAGCCCGGGCAAGGACTCAGAAGGAACTCATGACA
  GCACTTCGAGAGCTCAAGCTTCGACTGCCGCCAGAGCGCCGGGGCAAGGGCCGCTCTGGGACCCTGGCCA
  CGCTGCAGTACGCACTGGCCTGTGTCAAGCAGGTGCAGGCCAACCAGGAATACTACCAGCAGTGGAGCCT
  GGAGGAGGGCGAGCCTTGCTCCATGGACATGTCCACCTATACCCTGGAGGAGCTGGAGCACATCACGTCT
  GAGTACACACTTCAGAACCAGGATACCTTCTCAGTGGCTGTCTCCTTCCTGACGGGCCGAATCGTCTACA
  TTTCGGAGCAGGCAGCCGTCCTGCTGCGTTGCAAGCGGGACGTGTTCCGGGGTACCCGCTTCTCTGAGCT
  CCTGGCTCCCCAGGATGTGGGAGTCTTCTATGGTTCCACTGCTCCATCTCGCCTGCCCACCTGGGGCACA
  GGGGCCTCAGCAGGTTCAGGCCTCAGGGACTTTACCCAGGAGAAGTCCGTCTTCTGCCGTATCAGAGGAG
  GTCCTGACCGGGATCCAGGGCCTCGGTACCAGCCATTCCGCCTAACCCCGTATGTGACCAAGATCCGGGT
  CTCAGATGGGGCCCCTGCACAGCCGTGCTGCCTGCTGATTGCAGAGCGCATCCATTCGGGTTACGAAGCT
  CCCCGGATACCCCCTGACAAGAGGATTTTCACTACGCGGCACACACCCAGCTGCCTCTTCCAGGATGTGG
  ATGAAAGGGCTGCCCCCCTGCTGGGCTACCTGCCCCAGGACCTCCTGGGGGCCCCAGTGCTCCTGTTCCT
  GCATCCTGAGGACCGACCCCTCATGCTGGCTATCCACAAGAAGATTCTGCAGTTGGCGGGCCAGCCCTTT
  GACCACTCCCCTATCCGCTTCTGTGCCCGCAACGGGGAGTATGTCACCATGGACACCAGCTGGGCTGGCT
  TTGTGCACCCCTGGAGCCGCAAGGTAGCCTTCGTGTTGGGCCGCCACAAAGTACGCACGGCCCCCCTGAA
  TGAGGACGTGTTCACTCCCCCGGCCCCCAGCCCAGCTCCCTCCCTGGACACTGATATCCAGGAGCTGTCA
  GAGCAGATCCACCGGCTGCTGCTGCAGCCCGTCCACAGCCCCAGCCCCACGGGACTCTGTGGAGTCGGCG
  CCGTGACATCCCCAGGCCCTCTCCACAGCCCTGGGTCCTCCAGTGATAGCAACGGGGGTGATGCAGAGGG
  GCCTGGGCCTCCTGCGCCAGTGACTTTCCAACAGATCTGTAAGGATGTGCATCTGGTGAAGCACCAGGGC
  CAGCAGCTTTTTATTGAGTCTCGGGCCCGGCCTCAGTCCCGGCCCCGCCTCCCTGCTACAGGCACGTTCA
  AGGCCAAGGCCCTTCCCTGCCAATCCCCAGACCCAGAGCTGGAGGCGGGTTCTGCTCCCGTCCAGGCCCC
  ACTAGCCTTGGTCCCTGAGGAGGCCGAGAGGAAAGAAGCCTCCAGCTGCTCCTACCAGCAGATCAACTGC
  CTGGACAGCATCCTCAGGTACCTGGAGAGCTGCAACCTCCCCAGCACCACTAAGCGTAAATGTGCCTCCT
  CCTCCTCCTATACCACCTCCTCAGCCTCTGACGACGACAGGCAGAGGACAGGTCCAGTCTCTGTGGGGAC
  CAAGAAAGATCCGCCGTCAGCAGCGCTGTCTGGGGAGGGGGCCACCCCACGGAAGGAGCCAGTGGTGGGA
  GGCACCCTGAGCCCGCTCGCCCTGGCCAATAAGGCGGAGAGTGTGGTGTCCGTCACCAGTCAGTGTAGCT
  TCAGCTCCACCATCGTCCATGTGGGAGACAAGAAGCCCCCGGAGTCGGACATCATCATGATGGAGGACCT
  GCCTGGTCTAGCCCCAGGCCCAGCCCCCAGCCCAGCCCCCAGCCCCACAGTAGCCCCTGACCCAGCCCCA
  GACGCCTACCGTCCAGTGGGGCTGACCAAGGCCGTGCTGTCCCTGCACACGCAGAAGGAAGAGCAAGCCT
  TCCTCAGCCGCTTCCGAGACCTGGGCAGGCTGCGTGGACTCGACAGCTCTTCCACAGCTCCCTCAGCCCT
  TGGCGAGCGAGGCTGCCACCACGGCCCCGCACCCCCAAGCCGCCGACACCACTGCCGATCCAAAGCCAAG
  CGCTCACGCCACCACCAGAACCCTCGGGCTGAAGCGCCCTGCTATGTCTCACACCCCTCACCCGTGCCAC
  CCTCCACCCCCTGGCCCACCCCACCAGCCACTACCCCCTTCCCAGCGGTTGTCCAGCCCTACCCTCTCCC
  AGTGTTCTCTCCTCGAGGAGGCCCCCAGCCTCTTCCCCCTGCTCCCACATCTGTGCCCCCAGCTGCTTTC
  CCCGCCCCTTTGGTGACCCCAATGGTGGCCTTGGTGCTCCCTAACTATCTGTTCCCAACCCCATCCAGCT
  ATCCTTATGGGGCACTCCAGACCCCTGCTGAAGGGCCTCCCACTCCTGCCTCGCACTCCCCTTCTCCATC
  CTTGCCCGCCCTCCCCCCGAGTCCTCCTCACCGCCCGGACTCTCCACTGTTCAACTCGAGATGCAGCTCT
  CCACTCCAGCTCAATCTGCTGCAGCTGGAGGAGCTCCCCCGTGCTGAGGGGGCTGCTGTTGCAGGAGGCC
  CTGGGAGCAGTGCCGGGCCCCCACCTCCCAGTGCGGAGGCTGCTGAGCCAGAGGCCAGACTGGCGGAGGT
  CACTGAGTCCTCCAATCAGGACGCACTTTCCGGCTCCAGTGACCTGCTCGAACTTCTGCTGCAAGAGGAC
  TCGCGCTCCGGCACAGGCTCCGCAGCCTCGGGCTCCTTGGGCTCTGGCTTGGGCTCTGGGTCTGGTTCAG
  GCTCCCATGAAGGGGGCAGCACCTCAGCCAGCATCACTCGCAGCAGCCAGAGCAGCCACACAAGCAAATA
  CTTTGGCAGCATCGACTCTTCCGAGGCTGAGGCTGGGGCTGCTCGGGGCGGGGCTGAGCCTGGGGACCAG
  GTGATTAAGTACGTGCTCCAGGATCCCATTTGGCTGCTCATGGCCAATGCTGACCAGCGCGTCATGATGA
  CCTACCAGGTGCCCTCCAGGGACATGACCTCTGTGCTGAAGCAGGATCGGGAGCGGCTCCGAGCCATGCA
  GAAGCAGCAGCCTCGGTTTTCTGAGGACCAGCGGCGGGAACTGGGTGCTGTGCACTCCTGGGTCCGGAAG
  GGCCAACTGCCTCGGGCTCTTGATGTGATGGCCTGTGTGGACTGTGGGAGCAGCACCCAAGATCCTGGTC
  ACCCTGATGACCCACTCTTCTCAGAGCTGGATGGACTGGGGCTGGAGCCCATGGAAGAGGGTGGAGGCGA
  GCAGGGCAGCAGCGGTGGCGGCAGTGGTGAGGGAGAGGGCTGCGAGGAGGCCCAAGGCGGGGCCAAGGCT
  TCAAGCTCTCAGGACTTGGCTATGGAGGAGGAGGAAGAAGGCAGGAGCTCATCCAGTCCAGCCTTACCTA
  CAGCAGGAAACTGCACCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224777 representing NM_002616
  Red=Cloning site Green=Tags(s)
  
  MSGPLEGADGGGDPRPGESFCPGGVPSPGPPQHRPCPGPSLADDTDANSNGSSGNESNGHESRGASQRSS
  HSSSSGNGKDSALLETTESSKSTNSQSPSPPSSSIAYSLLSASSEQDNPSTSGCSSEQSARARTQKELMT
  ALRELKLRLPPERRGKGRSGTLATLQYALACVKQVQANQEYYQQWSLEEGEPCSMDMSTYTLEELEHITS
  EYTLQNQDTFSVAVSFLTGRIVYISEQAAVLLRCKRDVFRGTRFSELLAPQDVGVFYGSTAPSRLPTWGT
  GASAGSGLRDFTQEKSVFCRIRGGPDRDPGPRYQPFRLTPYVTKIRVSDGAPAQPCCLLIAERIHSGYEA
  PRIPPDKRIFTTRHTPSCLFQDVDERAAPLLGYLPQDLLGAPVLLFLHPEDRPLMLAIHKKILQLAGQPF
  DHSPIRFCARNGEYVTMDTSWAGFVHPWSRKVAFVLGRHKVRTAPLNEDVFTPPAPSPAPSLDTDIQELS
  EQIHRLLLQPVHSPSPTGLCGVGAVTSPGPLHSPGSSSDSNGGDAEGPGPPAPVTFQQICKDVHLVKHQG
  QQLFIESRARPQSRPRLPATGTFKAKALPCQSPDPELEAGSAPVQAPLALVPEEAERKEASSCSYQQINC
  LDSILRYLESCNLPSTTKRKCASSSSYTTSSASDDDRQRTGPVSVGTKKDPPSAALSGEGATPRKEPVVG
  GTLSPLALANKAESVVSVTSQCSFSSTIVHVGDKKPPESDIIMMEDLPGLAPGPAPSPAPSPTVAPDPAP
  DAYRPVGLTKAVLSLHTQKEEQAFLSRFRDLGRLRGLDSSSTAPSALGERGCHHGPAPPSRRHHCRSKAK
  RSRHHQNPRAEAPCYVSHPSPVPPSTPWPTPPATTPFPAVVQPYPLPVFSPRGGPQPLPPAPTSVPPAAF
  PAPLVTPMVALVLPNYLFPTPSSYPYGALQTPAEGPPTPASHSPSPSLPALPPSPPHRPDSPLFNSRCSS
  PLQLNLLQLEELPRAEGAAVAGGPGSSAGPPPPSAEAAEPEARLAEVTESSNQDALSGSSDLLELLLQED
  SRSGTGSAASGSLGSGLGSGSGSGSHEGGSTSASITRSSQSSHTSKYFGSIDSSEAEAGAARGGAEPGDQ
  VIKYVLQDPIWLLMANADQRVMMTYQVPSRDMTSVLKQDRERLRAMQKQQPRFSEDQRRELGAVHSWVRK
  GQLPRALDVMACVDCGSSTQDPGHPDDPLFSELDGLGLEPMEEGGGEQGSSGGGSGEGEGCEEAQGGAKA
  SSSQDLAMEEEEEGRSSSSPALPTAGNCTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002616
• ORF Size: 3870 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002616.1, NP_002607.1
• RefSeq Size: 4656 bp
• RefSeq ORF: 3873 bp
• Locus ID: 5187
• UniProt ID: O15534
• Cytogenetics: 17p13.1
• Protein Families: Druggable Genome
• Protein Pathways: Circadian rhythm - mammal
• MW: 136 kDa
• Gene Summary: This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene may increase the risk of getting certain cancers. Alternative splicing has been observed in this gene; however, these variants have not been fully described. [provided by RefSeq, Jan 2014]