TXNDC11 (NM_015914) Human Tagged ORF Clone

CAT#: RC224538

TXNDC11 (Myc-DDK-tagged)-Human thioredoxin domain containing 11 (TXNDC11)

Product Images

Circular map for RC224538

• 

  Western blot validation of overexpression lysate (Cat# LY414317) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224538 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TXNDC11 protein (Cat# TP324538). The protein was produced from HEK293T cells transfected with TXNDC11 cDNA clone (Cat# RC224538) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TXNDC11
• Synonyms: EFP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224538 representing NM_015914
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGAATGCGGAGGCCGCGGCGGCGGCAGCAGCAGCAGCGAGGACGCCGAGGACGAGGGAGGGGGCG
  GCGGCGGCCCCGCGGGCTCAGACTGCCTCAGCTCGAGCCCGACCCTGGCCACAGCGTCCTCGGCGGGCCG
  GCTCCGTCGCGGGCTGCGTGGCGCCTTCCTCATGGCGCGCCAGCGGCCCGAGCTGCTCTGCGGGGCCGTG
  GCGCTCGGCTGCGCGCTGCTCCTCGCCCTCAAGTTCACCTGCAGTCGAGCAAAAGATGTGATAATACCAG
  CAAAGCCACCTGTCAGCTTTTTCTCCTTGAGGTCTCCAGTCCTTGACCTCTTCCAGGGGCAGCTGGATTA
  TGCAGAGTACGTTCGACGGGATTCAGAGGTGGTACTGCTCTTCTTCTATGCCCCTTGGTGTGGACAGTCC
  ATCGCTGCCAGGGCAGAAATTGAGCAAGCAGCAAGTCGGCTTTCAGATCAGGTGTTGTTTGTGGCAATTA
  ACTGTTGGTGGAACCAGGGGAAATGCAGAAAACAGAAACACTTCTTTTATTTTCCTGTAATATATCTGTA
  TCATCGGAGTTTTGGACCAATCGAATACAAAGGCCCCATGAGTGCTGTTTACATTGAGAAGTTTGTCCGC
  CGGGTGATGAAACCACTTCTCTACATCCCATCTCAATCAGAATTACTAGATTTTCTCTCAAACTACGAGC
  CTGGAGTACTCGGGTACTTTGAGTTCAGTGGCTCACCCCAGCCTCCTGGTTATTTGACCTTCTTCACCTC
  AGCATTACATTCATTAAAGAAAGATTACCTAGGAACAGTACGATTTGGGGTTATCACAAATAAACATCTT
  GCGAAACTGGTATCCTTAGTACACTCTGGAAGTGTGTATTTACATAGACATTTCAACACATCACTTGTCT
  TCCCCAGGGAGGTCCTGAACTACACAGCTGAGAACATCTGTAAGTGGGCCTTAGAAAACCAGGAGACGCT
  CTTTCGGTGGCTGCGGCCACACGGAGGCAAGAGTCTCCTGCTGAATAACGAGCTGAAGAAAGGACCAGCG
  CTGTTTCTGTTCATACCTTTTAATCCCCTGGCCGAAAGTCATCCTTTAATAGACGAGATCACCGAAGTGG
  CCTTGGAGTACAACAACTGTCATGGGGACCAGGTGGTGGAGCGTCTCCTTCAGCACCTGCGGCGGGTGGA
  TGCTCCAGTGCTGGAGTCCCTGGCCCTGGAAGTGCCGGCACAGCTGCCAGACCCGCCAACGATCACAGCG
  TCCCCCTGCTGCAACACTGTGGTGCTGCCCCAGTGGCACTCCTTCTCCAGGACCCACAACGTCTGTGAAC
  TCTGTGTCAACCAGACCTCCGGGGGCATGAAGCCGAGCTCGGTCAGCGTGCCACAGTGCAGCTTTTTTGA
  AATGGCAGCAGCTCTGGATTCTTTCTACCTCAAGGAGCAGACCTTTTATCATGTGGCATCAGACAGCATA
  GAATGCAGCAATTTTTTAACTTCCTATAGCCCCTTCAGCTACTACACTGCATGTTGCAGGACCATAAGCA
  GGGGTGTGTCAGGCTTCATCGACTCTGAACAAGGTGTCTTTGAAGCCCCTACTGTTGCATTTTCTTCCCT
  TGAGAAGAAATGTGAGGTTGATGCCCCAAGCTCCGTTCCTCACATTGAGGAGAACAGGTATCTCTTTCCA
  GAAGTGGACATGACTAGCACAAACTTCACAGGCCTGAGCTGCAGAACCAACAAGACTCTCAACATCTACC
  TTTTGGATTCAAATTTGTTTTGGTTATATGCAGAGAGACTGGGTGCTCCGAGCTCCACTCAGGTGAAAGA
  ATTTGCGGCAATTGTTGACGTGAAAGAAGAATCTCATTACATCTTGGATCCAAAGCAAGCACTGATGAAG
  CTCACCCTAGAGTCTTTTATTCAAAACTTCAGCGTTCTCTATAGTCCCTTGAAAAGGCATCTCATTGGAA
  GTGGCTCTGCCCAGTTCCCGTCTCAGCATTTAATCACTGAAGTGACAACTGATACCTTTTGGGAAGTAGT
  CCTTCAAAAACAGGACGTTCTCCTGCTCTATTACGCTCCGTGGTGCGGCTTCTGTCCATCCCTCAATCAC
  ATCTTCATCCAGCTAGCTCGGAACCTGCCCATGGACACATTCACTGTGGCAAGGATTGACGTGTCTCAGA
  ATGACCTTCCTTGGGAATTTATGGTCGATCGTCTTCCTACTGTCTTGTTTTTTCCCTGCAACAGAAAGGA
  CCTAAGTGTGAAATACCCCGAAGACGTCCCCATCACCCTTCCAAACCTGTTGAGGTTCATTTTGCATCAC
  TCAGACCCTGCTTCCAGCCCCCAGAATGTGGCTAACTCTCCTACCAAGGAGTGTCTTCAGAGCGAGGCAG
  TCTTACAGCGGGGGCACATCTCCCACTTGGAGAGAGAGATCCAGAAACTGAGAGCAGAAATAAGCAGCCT
  CCAGCGAGCACAAGTGCAGGTGGAGTCCCAGCTCTCCAGTGCCCGCAGAGATGAGCACCGGCTGCGGCAG
  CAGCAGCGGGCCCTGGAAGAGCAGCACAGCCTGCTCCACGCACACAGTGAGCAGCTGCAGGCCCTCTATG
  AGCAGAAGACACGTGAGCTGCAGGAGCTGGCCCGCAAGCTGCAGGAGCTGGCCGATGCCTCAGAAAACCT
  CCTTACCGAGAACACGTGGCTCAAGATCCTGGTGGCGACCATGGAGAGGAAACTGGAGGGCAGGGATGGA
  GCTGAAAGCCTGGCGGCCCAGAGAGAGGTCCACCCCAAGCAGCCTGAGCCCTCAGCCACCCCCCAGCTCC
  CTGGCAGCTCCCCTCCACCTGCCAATGTCAGCGCCACACTGGTGTCTGAAAGGAATAAGGAGAACAGGAC
  AGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224538 representing NM_015914
  Red=Cloning site Green=Tags(s)
  
  MSECGGRGGGSSSSEDAEDEGGGGGGPAGSDCLSSSPTLATASSAGRLRRGLRGAFLMARQRPELLCGAV
  ALGCALLLALKFTCSRAKDVIIPAKPPVSFFSLRSPVLDLFQGQLDYAEYVRRDSEVVLLFFYAPWCGQS
  IAARAEIEQAASRLSDQVLFVAINCWWNQGKCRKQKHFFYFPVIYLYHRSFGPIEYKGPMSAVYIEKFVR
  RVMKPLLYIPSQSELLDFLSNYEPGVLGYFEFSGSPQPPGYLTFFTSALHSLKKDYLGTVRFGVITNKHL
  AKLVSLVHSGSVYLHRHFNTSLVFPREVLNYTAENICKWALENQETLFRWLRPHGGKSLLLNNELKKGPA
  LFLFIPFNPLAESHPLIDEITEVALEYNNCHGDQVVERLLQHLRRVDAPVLESLALEVPAQLPDPPTITA
  SPCCNTVVLPQWHSFSRTHNVCELCVNQTSGGMKPSSVSVPQCSFFEMAAALDSFYLKEQTFYHVASDSI
  ECSNFLTSYSPFSYYTACCRTISRGVSGFIDSEQGVFEAPTVAFSSLEKKCEVDAPSSVPHIEENRYLFP
  EVDMTSTNFTGLSCRTNKTLNIYLLDSNLFWLYAERLGAPSSTQVKEFAAIVDVKEESHYILDPKQALMK
  LTLESFIQNFSVLYSPLKRHLIGSGSAQFPSQHLITEVTTDTFWEVVLQKQDVLLLYYAPWCGFCPSLNH
  IFIQLARNLPMDTFTVARIDVSQNDLPWEFMVDRLPTVLFFPCNRKDLSVKYPEDVPITLPNLLRFILHH
  SDPASSPQNVANSPTKECLQSEAVLQRGHISHLEREIQKLRAEISSLQRAQVQVESQLSSARRDEHRLRQ
  QQRALEEQHSLLHAHSEQLQALYEQKTRELQELARKLQELADASENLLTENTWLKILVATMERKLEGRDG
  AESLAAQREVHPKQPEPSATPQLPGSSPPPANVSATLVSERNKENRTD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015914
• ORF Size: 2874 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015914.5, NP_056998.4
• RefSeq Size: 3047 bp
• RefSeq ORF: 2877 bp
• Locus ID: 51061
• UniProt ID: Q6PKC3
• Cytogenetics: 16p13.13
• Domains: thiored
• Protein Families: Druggable Genome
• MW: 107.3 kDa
• Gene Summary: May act as a redox regulator involved in DUOX proteins folding. The interaction with DUOX1 and DUOX2 suggest that it belongs to a multiprotein complex constituting the thyroid H(2)O(2) generating system. It is however not sufficient to assist DUOX1 and DUOX2 in H(2)O(2) generation.[UniProtKB/Swiss-Prot Function]