VPS18 (NM_020857) Human Tagged ORF Clone

CAT#: RC224536

VPS18 (Myc-DDK-tagged)-Human vacuolar protein sorting 18 homolog (S. cerevisiae) (VPS18)

Product Images

Circular map for RC224536

• 

  Western blot validation of overexpression lysate (Cat# LY412275) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224536 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VPS18
• Synonyms: PEP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224536 representing NM_020857
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCATCCTGGATGAGTACGAGAACTCGCTGTCCCGCTCGGCCGTCTTGCAGCCCGGCTGCCCTA
  GCGTGGGCATCCCCCACTCGGGGTATGTGAATGCCCAGCTGGAGAAGGAAGTGCCCATCTTCACAAAGCA
  GCGCATTGACTTCACCCCTTCCGAGCGCATTACCAGTCTTGTCGTCTCCAGCAATCAGCTGTGCATGAGC
  CTGGGCAAGGATACACTGCTCCGCATTGACTTGGGCAAGGCAAATGAGCCCAACCACGTGGAGCTGGGAC
  GTAAGGATGACGCAAAAGTTCACAAGATGTTCCTTGACCATACTGGCTCTCACCTGCTGATTGCCCTGAG
  CAGCACGGAGGTCCTCTACGTGAACCGAAATGGACAGAAGGTACGGCCACTAGCACGCTGGAAGGGGCAG
  CTGGTGGAGAGTGTGGGTTGGAACAAGGCACTGGGCACGGAGAGCAGCACAGGCCCCATCCTGGTCGGGA
  CTGCCCAAGGCCACATCTTTGAAGCAGAGCTCTCAGCCAGCGAAGGTGGGCTTTTCGGCCCTGCTCCGGA
  TCTCTACTTCCGCCCATTGTACGTGCTAAATGAAGAAGGGGGTCCAGCACCTGTGTGCTCCCTTGAGGCC
  GAGCGGGGCCCTGATGGGCGTAGCTTTGTTATTGCCACCACTCGGCAGCGCCTCTTCCAGTTCATAGGCC
  GAGCAGCAGAGGGGGCTGAGGCCCAGGGTTTCTCAGGGCTCTTTGCAGCTTACACGGACCACCCACCCCC
  ATTCCGTGAGTTTCCCAGCAACCTGGGCTACAGTGAGTTGGCCTTCTACACCCCCAAGCTGCGCTCCGCA
  CCCCGGGCCTTCGCCTGGATGATGGGGGATGGTGTGTTGTATGGGGCATTGGACTGTGGGCGCCCTGACT
  CTCTGCTGAGCGAGGAGCGAGTCTGGGAGTACCCAGAGGGGGTAGGGCCTGGGGCCAGCCCACCCCTAGC
  CATCGTCTTGACCCAGTTCCACTTCCTGCTGCTACTGGCAGACCGGGTGGAGGCAGTGTGCACACTGACC
  GGGCAGGTGGTGCTGCGGGATCACTTCCTGGAGAAATTTGGGCCGCTGAAGCACATGGTGAAGGACTCCT
  CCACAGGCCAGCTGTGGGCCTACACTGAGCGGGCTGTCTTCCGCTACCACGTGCAACGGGAGGCCCGAGA
  TGTCTGGCGCACCTATCTGGACATGAACCGCTTCGATCTGGCCAAAGAGTATTGTCGAGAGCGGCCCGAC
  TGCCTGGACACGGTCCTGGCCCGGGAGGCCGATTTCTGCTTTCGCCAGCGTCGCTACCTGGAGAGCGCAC
  GCTGCTATGCCCTGACCCAGAGCTACTTTGAGGAGATTGCCCTCAAGTTCCTGGAGGCCCGACAGGAGGA
  GGCTCTGGCTGAGTTCCTGCAGCGAAAACTGGCCAGTTTGAAGCCAGCCGAACGTACCCAGGCCACACTG
  CTGACCACCTGGCTGACAGAGCTCTACCTGAGCCGGCTTGGGGCTCTGCAGGGCGACCCAGAGGCCCTGA
  CTCTCTACCGAGAAACCAAGGAATGCTTTCGAACCTTCCTCAGCAGCCCCCGCCACAAAGAGTGGCTCTT
  TGCCAGCCGGGCCTCTATCCATGAGCTGCTCGCCAGTCATGGGGACACAGAACACATGGTGTACTTTGCA
  GTGATCATGCAGGACTATGAGCGGGTGGTGGCTTACCACTGTCAGCACGAGGCCTACGAGGAGGCCCTGG
  CCGTGCTCGCCCGCCACCGTGACCCCCAGCTCTTCTACAAGTTCTCACCCATCCTCATCCGTCACATCCC
  CCGCCAGCTTGTAGATGCCTGGATTGAGATGGGCAGCCGGCTGGATGCTCGTCAGCTCATTCCTGCCCTG
  GTGAACTACAGCCAGGGTGGTGAGGTCCAGCAGGTGAGCCAGGCCATCCGCTACATGGAGTTCTGCGTGA
  ACGTGCTGGGGGAGACTGAGCAGGCCATCCACAACTACCTGCTGTCACTGTATGCCCGTGGCCGGCCGGA
  CTCACTACTGGCCTATCTGGAGCAGGCTGGGGCCAGCCCCCACCGGGTGCATTACGACCTCAAGTATGCG
  CTGCGGCTCTGCGCCGAGCATGGCCACCACCGCGCTTGTGTCCATGTCTACAAGGTCCTAGAGCTGTATG
  AGGAGGCCGTGGACCTGGCCCTGCAGGTGGATGTGGACCTGGCCAAGCAGTGTGCAGACCTGCCTGAGGA
  GGATGAGGAATTGCGCAAGAAGCTGTGGCTGAAGATCGCACGGCACGTGGTGCAGGAAGAGGAAGATGTA
  CAGACAGCCATGGCTTGCCTGGCTAGCTGCCCCTTGCTCAAGATTGAGGATGTGCTGCCCTTCTTTCCTG
  ATTTCGTCACCATCGACCACTTCAAGGAGGCGATCTGCAGCTCACTTAAGGCCTACAACCACCACATCCA
  GGAGCTGCAGCGGGAGATGGAAGAGGCTACAGCCAGTGCCCAGCGCATCCGGCGAGACCTGCAGGAGCTG
  CGGGGCCGCTACGGCACTGTGGAGCCCCAGGACAAATGTGCCACCTGCGACTTCCCCCTGCTCAACCGCC
  CTTTTTACCTCTTCCTCTGTGGCCATATGTTCCATGCTGACTGCCTGCTGCAGGCTGTGCGACCTGGCCT
  GCCAGCCTACAAGCAGGCCCGGCTGGAGGAGCTGCAGAGGAAGCTGGGGGCTGCTCCACCCCCAGCCAAG
  GGCTCTGCCCGGGCCAAGGAGGCCGAGGGTGGGGCTGCCACGGCAGGGCCCAGCCGGGAACAGCTCAAGG
  CTGACCTGGATGAGTTGGTGGCCGCTGAGTGTGTGTACTGTGGGGAGCTGATGATCCGCTCTATCGACCG
  GCCGTTCATCGACCCCCAGCGCTACGAGGAGGAGCAGCTCAGTTGGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224536 representing NM_020857
  Red=Cloning site Green=Tags(s)
  
  MASILDEYENSLSRSAVLQPGCPSVGIPHSGYVNAQLEKEVPIFTKQRIDFTPSERITSLVVSSNQLCMS
  LGKDTLLRIDLGKANEPNHVELGRKDDAKVHKMFLDHTGSHLLIALSSTEVLYVNRNGQKVRPLARWKGQ
  LVESVGWNKALGTESSTGPILVGTAQGHIFEAELSASEGGLFGPAPDLYFRPLYVLNEEGGPAPVCSLEA
  ERGPDGRSFVIATTRQRLFQFIGRAAEGAEAQGFSGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSA
  PRAFAWMMGDGVLYGALDCGRPDSLLSEERVWEYPEGVGPGASPPLAIVLTQFHFLLLLADRVEAVCTLT
  GQVVLRDHFLEKFGPLKHMVKDSSTGQLWAYTERAVFRYHVQREARDVWRTYLDMNRFDLAKEYCRERPD
  CLDTVLAREADFCFRQRRYLESARCYALTQSYFEEIALKFLEARQEEALAEFLQRKLASLKPAERTQATL
  LTTWLTELYLSRLGALQGDPEALTLYRETKECFRTFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFA
  VIMQDYERVVAYHCQHEAYEEALAVLARHRDPQLFYKFSPILIRHIPRQLVDAWIEMGSRLDARQLIPAL
  VNYSQGGEVQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYARGRPDSLLAYLEQAGASPHRVHYDLKYA
  LRLCAEHGHHRACVHVYKVLELYEEAVDLALQVDVDLAKQCADLPEEDEELRKKLWLKIARHVVQEEEDV
  QTAMACLASCPLLKIEDVLPFFPDFVTIDHFKEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQEL
  RGRYGTVEPQDKCATCDFPLLNRPFYLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGAAPPPAK
  GSARAKEAEGGAATAGPSREQLKADLDELVAAECVYCGELMIRSIDRPFIDPQRYEEEQLSWL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020857
• ORF Size: 2919 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020857.3
• RefSeq Size: 3922 bp
• RefSeq ORF: 2922 bp
• Locus ID: 57617
• UniProt ID: Q9P253
• Cytogenetics: 15q15.1
• Domains: Pep3_Vps18
• MW: 110 kDa
• Gene Summary: Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps18 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. [provided by RefSeq, Jul 2008]