SLC5A7 (NM_021815) Human Tagged ORF Clone
CAT#: RC224138
SLC5A7 (Myc-DDK-tagged)-Human solute carrier family 5 (choline transporter), member 7 (SLC5A7)
Specifications
Product Data | |
Product Name | SLC5A7 (NM_021815) Human Tagged ORF Clone |
Symbol | SLC5A7 |
Synonyms | CHT; CHT1; hCHT; HMN7A |
Vector | pCMV6-Entry |
Sequence Data |
>RC224138 representing NM_021815
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTCCATGTGGAAGGACTGATAGCTATCATCGTGTTCTACCTTCTAATTTTGCTGGTTGGAATAT GGGCTGCCTGGAGAACCAAAAACAGTGGCAGCGCAGAAGAGCGCAGCGAAGCCATCATAGTTGGTGGCCG AGATATTGGTTTATTGGTTGGTGGATTTACCATGACAGCTACCTGGGTCGGAGGAGGGTATATCAATGGC ACAGCTGAAGCAGTTTATGTACCAGGTTATGGCCTAGCTTGGGCTCAGGCACCAATTGGATATTCTCTTA GTCTGATTTTAGGTGGCCTGTTCTTTGCAAAACCTATGCGTTCAAAGGGGTATGTGACCATGTTAGACCC GTTTCAGCAAATCTATGGAAAACGCATGGGCGGACTCCTGTTTATTCCTGCACTGATGGGAGAAATGTTC TGGGCTGCAGCAATTTTCTCTGCTTTGGGAGCCACCATCAGCGTGATCATCGATGTGGATATGCACATTT CTGTCATCATCTCTGCACTCATTGCCACTCTGTACACACTGGTGGGAGGGCTCTATTCTGTGGCCTACAC TGATGTCGTTCAGCTCTTTTGCATTTTTGTAGGGCTGTGGATCAGCGTCCCCTTTGCATTGTCACATCCT GCAGTCGCAGACATCGGGTTCACTGCTGTGCATGCCAAATACCAAAAGCCGTGGCTGGGAACTGTTGACT CATCTGAAGTCTACTCTTGGCTTGATAGTTTTCTGTTGTTGATGCTGGGTGGAATCCCATGGCAAGCATA CTTTCAGAGGGTTCTCTCTTCTTCCTCAGCCACCTATGCTCAAGTGCTGTCCTTCCTGGCAGCTTTCGGG TGCCTGGTGATGGCCATCCCAGCCATACTCATTGGGGCCATTGGAGCATCAACAGACTGGAACCAGACTG CATATGGGCTTCCAGATCCCAAGACTACAGAAGAGGCAGACATGATTTTACCAATTGTTCTGCAGTATCT CTGCCCTGTGTATATTTCTTTCTTTGGTCTTGGTGCAGTTTCTGCTGCTGTTATGTCATCAGCAGATTCT TCCATCTTGTCAGCAAGTTCCATGTTTGCACGGAACATCTACCAGCTTTCCTTCAGACAAAATGCTTCGG ACAAAGAAATCGTTTGGGTTATGCGAATCACAGTGTTTGTGTTTGGAGCATCTGCAACAGCCATGGCCTT GCTGACGAAAACTGTGTATGGGCTCTGGTACCTCAGTTCTGACCTTGTTTACATCGTTATCTTCCCCCAG CTGCTTTGTGTACTCTTTGTTAAGGGAACCAACACCTATGGGGCCGTGGCAGGTTATGTTTCTGGCCTCT TCCTGAGAATAACTGGAGGGGAGCCATATCTGTATCTTCAGCCCTTGATCTTCTACCCTGGCTATTACCC TGATGATAATGGTATATATAATCAGAAATTTCCATTTAAAACACTTGCCATGGTTACATCATTCTTAACC AACATTTGCATCTCCTATCTAGCCAAGTATCTATTTGAAAGTGGAACCTTGCCACCTAAATTAGATGTAT TTGATGCTGTTGTTGCAAGACACAGTGAAGAAAACATGGATAAGACAATTCTTGTCAAAAATGAAAATAT TAAATTAGATGAACTTGCACTTGTGAAGCCACGACAGAGCATGACCCTCAGCTCAACTTTCACCAATAAA GAGGCCTTCCTTGATGTTGATTCCAGTCCAGAAGGGTCTGGGACTGAAGATAATTTACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224138 representing NM_021815
Red=Cloning site Green=Tags(s) MAFHVEGLIAIIVFYLLILLVGIWAAWRTKNSGSAEERSEAIIVGGRDIGLLVGGFTMTATWVGGGYING TAEAVYVPGYGLAWAQAPIGYSLSLILGGLFFAKPMRSKGYVTMLDPFQQIYGKRMGGLLFIPALMGEMF WAAAIFSALGATISVIIDVDMHISVIISALIATLYTLVGGLYSVAYTDVVQLFCIFVGLWISVPFALSHP AVADIGFTAVHAKYQKPWLGTVDSSEVYSWLDSFLLLMLGGIPWQAYFQRVLSSSSATYAQVLSFLAAFG CLVMAIPAILIGAIGASTDWNQTAYGLPDPKTTEEADMILPIVLQYLCPVYISFFGLGAVSAAVMSSADS SILSASSMFARNIYQLSFRQNASDKEIVWVMRITVFVFGASATAMALLTKTVYGLWYLSSDLVYIVIFPQ LLCVLFVKGTNTYGAVAGYVSGLFLRITGGEPYLYLQPLIFYPGYYPDDNGIYNQKFPFKTLAMVTSFLT NICISYLAKYLFESGTLPPKLDVFDAVVARHSEENMDKTILVKNENIKLDELALVKPRQSMTLSSTFTNK EAFLDVDSSPEGSGTEDNLQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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Tag | Myc-DDK |
ACCN | NM_021815 |
ORF Size | 1740 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reference Data | |
RefSeq | NM_021815.1, NM_021815.2, NM_021815.3, NM_021815.4, NP_068587 |
RefSeq Size | 5158 |
RefSeq ORF | 1743 |
Locus ID | 60482 |
Cytogenetics | 2q12.3 |
Domains | SSF |
Protein Families | Transmembrane |
MW | 63 kDa |
Gene Summary | This gene encodes a sodium ion- and chloride ion-dependent high-affinity transporter that mediates choline uptake for acetylcholine synthesis in cholinergic neurons. The protein transports choline from the extracellular space into presynaptic terminals for synthesis into acetylcholine. Increased choline uptake results from increased density of this protein in synaptosomal plasma membranes in response to depolarization of cholinergic terminals. Dysfunction of cholinergic signaling has been implicated in various disorders including depression, attention-deficit disorder, and schizophrenia. An allelic variant of this gene is associated with autosomal dominant distal hereditary motor neuronopathy type VIIA. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
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Choline transporter mutations in severe congenital myasthenic syndrome disrupt transporter localization
,Wang, H;Salter, CG;Refai, O;Hardy, H;Barwick, KES;Akpulat, U;Kvarnung, M;Chioza, BA;Harlalka, G;Taylan, F;Sejersen, T;Wright, J;Zimmerman, HH;Karakaya, M;Stüve, B;Weis, J;Schara, U;Russell, MA;Abdul-Rahman, OA;Chilton, J;Blakely, RD;Baple, EL;Cirak, S;Crosby, AH;,
Brain 2017
,PubMed ID 29088354
[SLC5A7]
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Impaired Presynaptic High-Affinity Choline Transporter Causes a Congenital Myasthenic Syndrome with Episodic Apnea
,Bauché, S;O'Regan, S;Azuma, Y;Laffargue, F;McMacken, G;Sternberg, D;Brochier, G;Buon, C;Bouzidi, N;Topf, A;Lacène, E;Remerand, G;Beaufrere, AM;Pebrel-Richard, C;Thevenon, J;El Chehadeh-Djebbar, S;Faivre, L;Duffourd, Y;Ricci, F;Mongini, T;Fiorillo, C;Astrea, G;Burloiu, CM;Butoianu, N;Sandu, C;Servais, L;Bonne, G;Nelson, I;Desguerre, I;Nougues, MC;Bœuf, B;Romero, N;Laporte, J;Boland, A;Lechner, D;Deleuze, JF;Fontaine, B;Strochlic, L;Lochmuller, H;Eymard, B;Mayer, M;Nicole, S;,
Am. J. Hum. Genet. 2016
,PubMed ID 27569547
[CHT]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
cDNA Clone Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC224138L2 | Lenti ORF clone of Human solute carrier family 5 (choline transporter), member 7 (SLC5A7), mGFP tagged |
USD 860.00 |
|
SC112868 | SLC5A7 (untagged)-Human solute carrier family 5 (choline transporter), member 7 (SLC5A7) |
USD 740.00 |
|
RC224138L3 | Lenti ORF clone of Human solute carrier family 5 (choline transporter), member 7 (SLC5A7) , Myc-DDK-tagged |
USD 790.00 |
|
RC224138L2V | Lenti ORF particles, SLC5A7 (mGFP-tagged) - Human solute carrier family 5 (choline transporter), member 7 (SLC5A7), 200ul, >10^7 TU/mL |
USD 1,110.00 |
|
RC224138L1V | Lenti ORF particles, SLC5A7 (Myc-DDK tagged) - Human solute carrier family 5 (choline transporter), member 7 (SLC5A7) , 200ul, >10^7 TU/mL |
USD 1,000.00 |
|
RG224138 | SLC5A7 (GFP-tagged) - Human solute carrier family 5 (choline transporter), member 7 (SLC5A7) |
USD 650.00 |
|
RC224138L4V | Lenti ORF particles, SLC5A7 (mGFP-tagged) - Human solute carrier family 5 (choline transporter), member 7 (SLC5A7), 200ul, >10^7 TU/mL |
USD 1,110.00 |
|
RC224138L1 | Lenti ORF clone of Human solute carrier family 5 (choline transporter), member 7 (SLC5A7) , Myc-DDK-tagged |
USD 790.00 |
|
RC224138L3V | Lenti ORF particles, SLC5A7 (Myc-DDK tagged) - Human solute carrier family 5 (choline transporter), member 7 (SLC5A7) , 200ul, >10^7 TU/mL |
USD 1,000.00 |
|
RC224138L4 | Lenti ORF clone of Human solute carrier family 5 (choline transporter), member 7 (SLC5A7), mGFP tagged |
USD 860.00 |
USD 299.00
USD 550.00