MAD1 (MAD1L1) (NM_001013836) Human Tagged ORF Clone

CAT#: RC222718

MAD1L1 (Myc-DDK-tagged)-Human MAD1 mitotic arrest deficient-like 1 (yeast) (MAD1L1), transcript variant 2

Product Images

Circular map for RC222718

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  Western blot validation of overexpression lysate (Cat# LY423038) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222896 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MAD1L1 protein (Cat# TP322718). The protein was produced from HEK293T cells transfected with MAD1L1 cDNA clone (Cat# RC222718) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MAD1
• Synonyms: MAD1; PIG9; TP53I9; TXBP181
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222718 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGACCTGGGGGAAAACACCATGGTTTTATCCACCCTGAGATCTTTGAACAACTTCATCTCTCAGC
  GTGTGGAGGGAGGCTCTGGACTGGATATTTCTACCTCGGCCCCAGGTTCTCTGCAGATGCAGTACCAGCA
  GAGCATGCAGCTGGAGGAAAGAGCAGAGCAGATCCGTTCGAAGTCCCACCTCATCCAGGTGGAGCGGGAG
  AAAATGCAGATGGAGCTGAGTCACAAGAGGGCTCGAGTGGAGCTGGAGAGAGCAGCCAGCACCAGTGCCA
  GGAACTACGAGCGTGAGGTCGACCGCAACCAGGAGCTCCTGACGCGCATCCGGCAGCTTCAGGAGCGGGA
  GGCCGGGGCGGAGGAGAAGATGCAGGAGCAGCTGGAGCGCAACAGGCAGTGTCAGCAGAACTTGGATGCT
  GCCAGCAAGAGGCTGCGTGAGAAAGAGGACAGTCTGGCCCAGGCTGGCGAGACCATCAACGCACTGAAGG
  GGAGGATCTCGGAACTGCAGTGGAGCGTGATGGACCAGGAGATGCGGGTGAAGCGCCTGGAGTCGGAGAA
  GCAGGAGCTGCAGGAGCAGCTGGACCTGCAACACAAAAAATGCCAGGAAGCCAATCAGAAAATCCAGGAA
  CTCCAGGCCAGCCAAGAAGCAAGAGCAGACCACGAGCAGCAGATTAAGGATCTGGAGCAGAAGCTGTCCC
  TGCAAGAGCAGGATGCAGCGATTGTGAAGAACATGAAGTCTGAGCTGGTACGGCTCCCTAGGCTGGAACG
  GGAGCTGAAGCAGCTGCGGGAGGAGAGCGCGCACCTGCGGGAGATGAGAGAGACCAACGGGCTGCTCCAG
  GAAGAGCTGGAAGGGCTGCAGAGGAAGCTGGGGCGCCAGGAGAAGATGCAGGAGACGCTGGTTGGCTTGG
  AGCTGGAGAACGAGAGGCTGCTGGCCAAGCTGCAAAGCTGGGAGAGACTGGACCAGACCATGGGCCTGAG
  CATCAGGACTCCAGAAGACCTTTCCAGATTCGTGGTTGAGCTGCAGCAGAGGGAGCTTGCCTTGAAGGAC
  AAGAACAGCGCCGTCACCAGCAGCGCCCGGGGGCTGGAGAAGGCCAGGCAGCAGCTGCAGGAGGAGCTCC
  GGCAGGTCAGCGGCCAGCTGTTGGAGGAGAGGAAGAAGCGCGAGACCCACGAGGCGCTGGCCCGGAGGCT
  CCAGAAACGGGTCCTGCTGCTCACCAAGGAGCGGGACGGTATGCGGGCCATCCTGGGGTCCTACGACAGC
  GAGCTGACCCCGGCCGAGTACTCACCCCAGCTGACGCGGCGCATGCGGGAGGCTGAGGATATGGTGCAGA
  AGGTGCACAGCCACAGCGCCGAGATGGAGGCTCAGCTGTCGCAGGCCCTGGAGGAGCTGGGAGGCCAGAA
  ACAAAGAGCAGACATGCTGGAGATGGAGCTGAAGATGCTGAAGTCTCAGTCCAGCTCTGCCGAACAGAGC
  TTCCTGTTCTCCAGGGAGGAGGCGGACACGCTCAGGTTGAAGGTCGAGGAGCTGGAAGGCGAGCGGAGTC
  GGCTGGAGGAGGAAAAGAGGATGCTGGAGGCACAGCTGGAGCGGCGAGCTCTGCAGGGTGACTATGACCA
  GAGCAGGACCAAAGTGCTGCACATGAGCCTGAACCCCACCAGTGTGGCCAGGCAGCGCCTGCGCGAGGAC
  CACAGCCAGCTGCAGGCGGAGTGCGAGCGACTGCGCGGGCTCCTGCGCGCCATGGAGAGAGGAGGCACCG
  TCCCAGCCGACCTTGAGGCTGCCGCCGCGAGTCTGCCATCGTCCAAGGAGGTGGCAGAGCTGAAGAAGCA
  GGTGGAGAGTGCCGAGCTGAAGAACCAGCGGCTCAAGGAGGTTTTCCAGACCAAGATCCAGGAGTTCCGC
  AAGGCCTGCTACACGCTCACCGGCTACCAGATCGACATCACCACGGAGAACCAGTACCGGCTGACCTCGC
  TGTACGCCGAGCACCCAGGCGACTGCCTCATCTTCAAGGCCACCAGCCCCTCGGGTTCCAAGATGCAGCT
  ACTGGAGACAGAGTTCTCACACACCGTGGGCGAGCTCATCGAGGTGCACCTGCGGCGCCAGGACAGCATC
  CCTGCCTTCCTCAGCTCGCTCACCCTCGAGCTCTTCAGCCGCCAGACCGTGGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222718 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEDLGENTMVLSTLRSLNNFISQRVEGGSGLDISTSAPGSLQMQYQQSMQLEERAEQIRSKSHLIQVERE
  KMQMELSHKRARVELERAASTSARNYEREVDRNQELLTRIRQLQEREAGAEEKMQEQLERNRQCQQNLDA
  ASKRLREKEDSLAQAGETINALKGRISELQWSVMDQEMRVKRLESEKQELQEQLDLQHKKCQEANQKIQE
  LQASQEARADHEQQIKDLEQKLSLQEQDAAIVKNMKSELVRLPRLERELKQLREESAHLREMRETNGLLQ
  EELEGLQRKLGRQEKMQETLVGLELENERLLAKLQSWERLDQTMGLSIRTPEDLSRFVVELQQRELALKD
  KNSAVTSSARGLEKARQQLQEELRQVSGQLLEERKKRETHEALARRLQKRVLLLTKERDGMRAILGSYDS
  ELTPAEYSPQLTRRMREAEDMVQKVHSHSAEMEAQLSQALEELGGQKQRADMLEMELKMLKSQSSSAEQS
  FLFSREEADTLRLKVEELEGERSRLEEEKRMLEAQLERRALQGDYDQSRTKVLHMSLNPTSVARQRLRED
  HSQLQAECERLRGLLRAMERGGTVPADLEAAAASLPSSKEVAELKKQVESAELKNQRLKEVFQTKIQEFR
  KACYTLTGYQIDITTENQYRLTSLYAEHPGDCLIFKATSPSGSKMQLLETEFSHTVGELIEVHLRRQDSI
  PAFLSSLTLELFSRQTVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013836
• ORF Size: 2154 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013836.2
• RefSeq Size: 2717 bp
• RefSeq ORF: 2157 bp
• Locus ID: 8379
• UniProt ID: Q9Y6D9
• Cytogenetics: 7p22.3
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle
• MW: 83.1 kDa
• Gene Summary: MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]