TAL1 (NM_003189) Human Tagged ORF Clone

CAT#: RC222628

TAL1 (Myc-DDK-tagged)-Human T-cell acute lymphocytic leukemia 1 (TAL1)

Product Images

Circular map for RC222628

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TAL1 (Cat# RC222628, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TAL1(Cat# TA503922). Positive lysates LY418842 (100ug) and LC418842 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY418842) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222628 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TAL1
• Synonyms: bHLHa17; SCL; tal-1; TCL5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222628 representing NM_003189
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGAGCGGCCGCCGAGCGAGGCGGCTCGCAGTGACCCCCAGCTAGAGGGACGGGACGCGGCCGAGG
  CCAGCATGGCCCCCCCGCACCTGGTCCTGCTGAACGGCGTCGCCAAGGAGACGAGCCGCGCGGCCGCAGC
  GGAGCCCCCAGTCATCGAACTGGGCGCGCGCGGAGGCCCGGGGGGCGGCCCTGCCGGTGGGGGCGGCGCC
  GCGAGAGACTTAAAGGGCCGCGACGCGGCGACGGCCGAAGCGCGCCATCGGGTGCCCACCACCGAGCTGT
  GCAGACCTCCCGGGCCCGCCCCGGCCCCCGCGCCCGCCTCGGTTACAGCGGAGCTGCCCGGCGACGGCCG
  CATGGTGCAGCTGAGTCCTCCCGCGCTGGCTGCCCCCGCCGCCCCCGGCCGCGCGCTGCTCTACAGCCTC
  AGCCAGCCGCTGGCCTCTCTCGGCAGCGGGTTCTTTGGGGAGCCGGATGCCTTCCCTATGTTCACCACCA
  ACAATCGAGTGAAGAGGAGACCTTCCCCCTATGAGATGGAGATTACTGATGGTCCCCACACCAAAGTTGT
  GCGGCGTATCTTCACCAACAGCCGGGAGCGATGGCGGCAGCAGAATGTGAACGGGGCCTTTGCCGAGCTC
  CGCAAGCTGATCCCCACACATCCCCCGGACAAGAAGCTCAGCAAGAATGAGATCCTCCGCCTGGCCATGA
  AGTATATCAACTTCTTGGCCAAGCTGCTCAATGACCAGGAGGAGGAGGGCACCCAGCGGGCCAAGACTGG
  CAAGGACCCTGTGGTGGGGGCTGGTGGGGGTGGAGGTGGGGGAGGGGGTGGCGCGCCCCCAGATGACCTC
  CTGCAAGACGTGCTTTCCCCCAACTCCAGCTGCGGCAGCTCCCTGGATGGGGCAGCCAGCCCGGACAGCT
  ACACGGAGGAGCCCGCGCCCAAGCACACGGCCCGCAGCCTCCATCCTGCCATGCTGCCTGCCGCCGATGG
  AGCCGGCCCTCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222628 representing NM_003189
  Red=Cloning site Green=Tags(s)
  
  MTERPPSEAARSDPQLEGRDAAEASMAPPHLVLLNGVAKETSRAAAAEPPVIELGARGGPGGGPAGGGGA
  ARDLKGRDAATAEARHRVPTTELCRPPGPAPAPAPASVTAELPGDGRMVQLSPPALAAPAAPGRALLYSL
  SQPLASLGSGFFGEPDAFPMFTTNNRVKRRPSPYEMEITDGPHTKVVRRIFTNSRERWRQQNVNGAFAEL
  RKLIPTHPPDKKLSKNEILRLAMKYINFLAKLLNDQEEEGTQRAKTGKDPVVGAGGGGGGGGGGAPPDDL
  LQDVLSPNSSCGSSLDGAASPDSYTEEPAPKHTARSLHPAMLPAADGAGPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003189
• ORF Size: 993 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003189.5
• RefSeq Size: 5000 bp
• RefSeq ORF: 996 bp
• Locus ID: 6886
• UniProt ID: P17542
• Cytogenetics: 1p33
• Domains: HLH
• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• MW: 34.1 kDa
• Gene Summary: Implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation. Serves as a positive regulator of erythroid differentiation (By similarity).[UniProtKB/Swiss-Prot Function]