TAL1 Mouse Monoclonal Antibody [Clone ID: OTI5H1]

CAT#: TA503922

TAL1 mouse monoclonal antibody, clone OTI5H1 (formerly 5H1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TAL1 (Cat# RC222628, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TAL1(Cat# TA503922). Positive lysates LY418842 (100ug) and LC418842 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-TAL1 monoclonal antibody at 1:200.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503922)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503922)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503922)

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  Anti-TAL1 mouse monoclonal antibody (TA503922) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TAL1 (RC222628).

• HEK293T cells transfected with either RC222628 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TAL1 antibody (TA503922), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI5H1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:200 - 1:1000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TAL1(NP_003180) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.88 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.1 kDa
• Gene Name: TAL bHLH transcription factor 1, erythroid differentiation factor
• Database Link:
  NP_003180
  Entrez Gene 21349 MouseEntrez Gene 313507 RatEntrez Gene 6886 Human
  UniProt ID P17542
• Synonyms: bHLHa17; SCL; tal-1; TCL5

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors