LRRC8C (NM_032270) Human Tagged ORF Clone

CAT#: RC222603

LRRC8C (Myc-DDK-tagged)-Human leucine rich repeat containing 8 family, member C (LRRC8C)

Product Images

Circular map for RC222603

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  Western blot validation of overexpression lysate (Cat# LY410256) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222603 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified LRRC8C protein (Cat# TP322603). The protein was produced from HEK293T cells transfected with LRRC8C cDNA clone (Cat# RC222603) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LRRC8C
• Synonyms: AD158; FAD158
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222603 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCCCGTGACAGAATTCCGGCAGTTCTCTGAGCAGCAGCCTGCCTTCCGAGTGCTGAAGCCATGGT
  GGGATGTGTTTACCGATTACCTCTCAGTAGCCATGCTCATGATCGGCGTGTTTGGATGTACTTTACAGGT
  CATGCAAGACAAGATAATCTGCCTTCCGAAAAGAGTGCAGCCTGCTCAGAACCACTCTTCCCTTTCGAAT
  GTCTCTCAAGCAGTTGCCAGTACCACTCCACTGCCTCCACCTAAACCATCTCCTGCTAACCCCATCACTG
  TGGAAATGAAAGGCCTGAAGACAGATTTGGACCTTCAGCAGTACAGCTTTATAAATCAGATGTGTTATGA
  GCGAGCCCTCCACTGGTATGCCAAGTATTTCCCTTACCTTGTCCTCATCCATACCCTGGTCTTTATGCTC
  TGCAGTAACTTTTGGTTCAAATTCCCTGGTTCCAGCTCCAAAATAGAACATTTCATCTCCATTCTGGGGA
  AGTGTTTTGACTCTCCTTGGACCACACGGGCTTTATCTGAAGTGTCTGGGGAGGACTCAGAAGAAAAGGA
  CAACAGGAAGAACAACATGAACAGGTCCAACACCATCCAATCTGGTCCAGAAGGCAGCCTGGTCAACTCT
  CAGTCTTTAAAGTCCATTCCTGAGAAGTTTGTAGTTGATAAATCCACTGCAGGGGCTCTGGATAAAAAGG
  AAGGTGAGCAGGCTAAGGCCTTATTTGAGAAGGTGAAGAAGTTCAGGCTGCATGTGGAAGAAGGTGATAT
  TCTATATGCCATGTATGTTCGCCAGACTGTACTTAAAGTTATCAAATTCCTAATCATCATTGCATATAAT
  AGTGCTCTGGTTTCCAAGGTCCAGTTTACAGTGGACTGTAATGTGGACATTCAGGACATGACTGGATATA
  AAAACTTTTCTTGCAATCATACCATGGCACACTTGTTCTCAAAACTGTCCTTTTGCTATCTGTGCTTTGT
  TAGTATCTATGGATTGACGTGCCTTTATACCTTATACTGGCTGTTCTACCGTTCTCTACGGGAATATTCC
  TTTGAGTATGTCCGTCAGGAGACTGGAATTGATGATATTCCAGATGTGAAAAATGACTTTGCTTTTATGC
  TTCATATGATAGATCAGTATGACCCTCTCTATTCCAAGAGATTTGCAGTGTTCCTGTCTGAAGTCAGTGA
  AAACAAATTAAAGCAGCTGAACTTAAATAACGAATGGACTCCTGATAAACTGAGGCAGAAGCTACAGACA
  AATGCCCATAATCGACTGGAATTGCCTCTTATCATGCTCTCTGGCCTTCCAGACACTGTTTTTGAAATCA
  CAGAGTTGCAATCTCTAAAACTTGAAATCATTAAGAACGTAATGATACCAGCCACCATTGCACAGCTAGA
  CAATCTTCAAGAGCTCTCTCTGCACCAGTGTTCTGTCAAAATCCACAGTGCGGCGCTCTCTTTCCTGAAG
  GAAAACCTCAAGGTCTTGAGCGTCAAGTTTGATGACATGAGGGAACTCCCCCCCTGGATGTATGGGCTCC
  GAAATCTGGAAGAGCTGTACCTAGTTGGCTCTCTAAGTCATGATATTTCCAGAAATGTCACCCTTGAGTC
  TCTGCGGGATCTCAAAAGCCTTAAAATTCTCTCTATCAAAAGCAACGTTTCCAAAATCCCTCAGGCAGTG
  GTTGATGTTTCCAGCCATCTCCAGAAGATGTGCATACATAATGATGGCACCAAGCTGGTGATGCTCAACA
  ACTTAAAGAAGATGACCAATCTGACAGAGCTGGAGCTGGTCCACTGTGACCTGGAGCGTATTCCTCATGC
  TGTGTTCAGCCTACTCAGCCTCCAGGAATTGGACCTGAAGGAAAACAATCTGAAATCTATAGAAGAAATC
  GTTAGCTTTCAGCACTTAAGAAAGTTGACAGTGCTAAAACTGTGGCATAACAGCATCACCTACATCCCAG
  AGCATATAAAGAAACTCACCAGCCTGGAACGCCTGTCCTTTAGTCACAATAAAATAGAGGTGCTGCCTTC
  CCACCTCTTCCTATGCAACAAGATCCGATACTTGGACTTATCGTACAATGACATTCGATTTATCCCCCCT
  GAAATTGGAGTTCTACAAAGTTTACAGTATTTTTCCATCACATGTAACAAAGTGGAAAGCCTTCCAGATG
  AACTCTACTTCTGCAAGAAACTTAAAACTCTGAAGATTGGAAAAAACAGCCTATCTGTACTTTCACCGAA
  AATTGGAAATTTGCTATTTCTTTCCTACTTAGATGTAAAAGGTAATCACTTTGAAATCCTCCCTCCTGAA
  CTGGGTGACTGTCGGGCTCTGAAGCGAGCTGGTTTAGTTGTAGAAGATGCTCTGTTTGAAACTCTGCCTT
  CTGACGTCCGGGAGCAAATGAAAACAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222603 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MIPVTEFRQFSEQQPAFRVLKPWWDVFTDYLSVAMLMIGVFGCTLQVMQDKIICLPKRVQPAQNHSSLSN
  VSQAVASTTPLPPPKPSPANPITVEMKGLKTDLDLQQYSFINQMCYERALHWYAKYFPYLVLIHTLVFML
  CSNFWFKFPGSSSKIEHFISILGKCFDSPWTTRALSEVSGEDSEEKDNRKNNMNRSNTIQSGPEGSLVNS
  QSLKSIPEKFVVDKSTAGALDKKEGEQAKALFEKVKKFRLHVEEGDILYAMYVRQTVLKVIKFLIIIAYN
  SALVSKVQFTVDCNVDIQDMTGYKNFSCNHTMAHLFSKLSFCYLCFVSIYGLTCLYTLYWLFYRSLREYS
  FEYVRQETGIDDIPDVKNDFAFMLHMIDQYDPLYSKRFAVFLSEVSENKLKQLNLNNEWTPDKLRQKLQT
  NAHNRLELPLIMLSGLPDTVFEITELQSLKLEIIKNVMIPATIAQLDNLQELSLHQCSVKIHSAALSFLK
  ENLKVLSVKFDDMRELPPWMYGLRNLEELYLVGSLSHDISRNVTLESLRDLKSLKILSIKSNVSKIPQAV
  VDVSSHLQKMCIHNDGTKLVMLNNLKKMTNLTELELVHCDLERIPHAVFSLLSLQELDLKENNLKSIEEI
  VSFQHLRKLTVLKLWHNSITYIPEHIKKLTSLERLSFSHNKIEVLPSHLFLCNKIRYLDLSYNDIRFIPP
  EIGVLQSLQYFSITCNKVESLPDELYFCKKLKTLKIGKNSLSVLSPKIGNLLFLSYLDVKGNHFEILPPE
  LGDCRALKRAGLVVEDALFETLPSDVREQMKTE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032270
• ORF Size: 2409 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032270.4
• RefSeq Size: 7207 bp
• RefSeq ORF: 2412 bp
• Locus ID: 84230
• UniProt ID: Q8TDW0
• Cytogenetics: 1p22.2
• Domains: LRR, LRR_TYP, LRR_PS
• Protein Families: Transmembrane
• MW: 92.4 kDa
• Gene Summary: Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes. The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine. Plays a redundant role in the efflux of amino acids, such as aspartate and glutamate, in response to osmotic stress. Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition.[UniProtKB/Swiss-Prot Function]