LRRC8C (NM_032270) Human Tagged ORF Clone

CAT#: RC222603

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LRRC8C (Myc-DDK-tagged)-Human leucine rich repeat containing 8 family, member C (LRRC8C)



  "NM_032270" in other vectors (6)

Reconstitution Protocol

USD 735.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol LRRC8C
Synonyms AD158; FAD158
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC222603 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTCCCGTGACAGAATTCCGGCAGTTCTCTGAGCAGCAGCCTGCCTTCCGAGTGCTGAAGCCATGGT
GGGATGTGTTTACCGATTACCTCTCAGTAGCCATGCTCATGATCGGCGTGTTTGGATGTACTTTACAGGT
CATGCAAGACAAGATAATCTGCCTTCCGAAAAGAGTGCAGCCTGCTCAGAACCACTCTTCCCTTTCGAAT
GTCTCTCAAGCAGTTGCCAGTACCACTCCACTGCCTCCACCTAAACCATCTCCTGCTAACCCCATCACTG
TGGAAATGAAAGGCCTGAAGACAGATTTGGACCTTCAGCAGTACAGCTTTATAAATCAGATGTGTTATGA
GCGAGCCCTCCACTGGTATGCCAAGTATTTCCCTTACCTTGTCCTCATCCATACCCTGGTCTTTATGCTC
TGCAGTAACTTTTGGTTCAAATTCCCTGGTTCCAGCTCCAAAATAGAACATTTCATCTCCATTCTGGGGA
AGTGTTTTGACTCTCCTTGGACCACACGGGCTTTATCTGAAGTGTCTGGGGAGGACTCAGAAGAAAAGGA
CAACAGGAAGAACAACATGAACAGGTCCAACACCATCCAATCTGGTCCAGAAGGCAGCCTGGTCAACTCT
CAGTCTTTAAAGTCCATTCCTGAGAAGTTTGTAGTTGATAAATCCACTGCAGGGGCTCTGGATAAAAAGG
AAGGTGAGCAGGCTAAGGCCTTATTTGAGAAGGTGAAGAAGTTCAGGCTGCATGTGGAAGAAGGTGATAT
TCTATATGCCATGTATGTTCGCCAGACTGTACTTAAAGTTATCAAATTCCTAATCATCATTGCATATAAT
AGTGCTCTGGTTTCCAAGGTCCAGTTTACAGTGGACTGTAATGTGGACATTCAGGACATGACTGGATATA
AAAACTTTTCTTGCAATCATACCATGGCACACTTGTTCTCAAAACTGTCCTTTTGCTATCTGTGCTTTGT
TAGTATCTATGGATTGACGTGCCTTTATACCTTATACTGGCTGTTCTACCGTTCTCTACGGGAATATTCC
TTTGAGTATGTCCGTCAGGAGACTGGAATTGATGATATTCCAGATGTGAAAAATGACTTTGCTTTTATGC
TTCATATGATAGATCAGTATGACCCTCTCTATTCCAAGAGATTTGCAGTGTTCCTGTCTGAAGTCAGTGA
AAACAAATTAAAGCAGCTGAACTTAAATAACGAATGGACTCCTGATAAACTGAGGCAGAAGCTACAGACA
AATGCCCATAATCGACTGGAATTGCCTCTTATCATGCTCTCTGGCCTTCCAGACACTGTTTTTGAAATCA
CAGAGTTGCAATCTCTAAAACTTGAAATCATTAAGAACGTAATGATACCAGCCACCATTGCACAGCTAGA
CAATCTTCAAGAGCTCTCTCTGCACCAGTGTTCTGTCAAAATCCACAGTGCGGCGCTCTCTTTCCTGAAG
GAAAACCTCAAGGTCTTGAGCGTCAAGTTTGATGACATGAGGGAACTCCCCCCCTGGATGTATGGGCTCC
GAAATCTGGAAGAGCTGTACCTAGTTGGCTCTCTAAGTCATGATATTTCCAGAAATGTCACCCTTGAGTC
TCTGCGGGATCTCAAAAGCCTTAAAATTCTCTCTATCAAAAGCAACGTTTCCAAAATCCCTCAGGCAGTG
GTTGATGTTTCCAGCCATCTCCAGAAGATGTGCATACATAATGATGGCACCAAGCTGGTGATGCTCAACA
ACTTAAAGAAGATGACCAATCTGACAGAGCTGGAGCTGGTCCACTGTGACCTGGAGCGTATTCCTCATGC
TGTGTTCAGCCTACTCAGCCTCCAGGAATTGGACCTGAAGGAAAACAATCTGAAATCTATAGAAGAAATC
GTTAGCTTTCAGCACTTAAGAAAGTTGACAGTGCTAAAACTGTGGCATAACAGCATCACCTACATCCCAG
AGCATATAAAGAAACTCACCAGCCTGGAACGCCTGTCCTTTAGTCACAATAAAATAGAGGTGCTGCCTTC
CCACCTCTTCCTATGCAACAAGATCCGATACTTGGACTTATCGTACAATGACATTCGATTTATCCCCCCT
GAAATTGGAGTTCTACAAAGTTTACAGTATTTTTCCATCACATGTAACAAAGTGGAAAGCCTTCCAGATG
AACTCTACTTCTGCAAGAAACTTAAAACTCTGAAGATTGGAAAAAACAGCCTATCTGTACTTTCACCGAA
AATTGGAAATTTGCTATTTCTTTCCTACTTAGATGTAAAAGGTAATCACTTTGAAATCCTCCCTCCTGAA
CTGGGTGACTGTCGGGCTCTGAAGCGAGCTGGTTTAGTTGTAGAAGATGCTCTGTTTGAAACTCTGCCTT
CTGACGTCCGGGAGCAAATGAAAACAGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC222603 protein sequence
Red=Cloning site Green=Tags(s)

MIPVTEFRQFSEQQPAFRVLKPWWDVFTDYLSVAMLMIGVFGCTLQVMQDKIICLPKRVQPAQNHSSLSN
VSQAVASTTPLPPPKPSPANPITVEMKGLKTDLDLQQYSFINQMCYERALHWYAKYFPYLVLIHTLVFML
CSNFWFKFPGSSSKIEHFISILGKCFDSPWTTRALSEVSGEDSEEKDNRKNNMNRSNTIQSGPEGSLVNS
QSLKSIPEKFVVDKSTAGALDKKEGEQAKALFEKVKKFRLHVEEGDILYAMYVRQTVLKVIKFLIIIAYN
SALVSKVQFTVDCNVDIQDMTGYKNFSCNHTMAHLFSKLSFCYLCFVSIYGLTCLYTLYWLFYRSLREYS
FEYVRQETGIDDIPDVKNDFAFMLHMIDQYDPLYSKRFAVFLSEVSENKLKQLNLNNEWTPDKLRQKLQT
NAHNRLELPLIMLSGLPDTVFEITELQSLKLEIIKNVMIPATIAQLDNLQELSLHQCSVKIHSAALSFLK
ENLKVLSVKFDDMRELPPWMYGLRNLEELYLVGSLSHDISRNVTLESLRDLKSLKILSIKSNVSKIPQAV
VDVSSHLQKMCIHNDGTKLVMLNNLKKMTNLTELELVHCDLERIPHAVFSLLSLQELDLKENNLKSIEEI
VSFQHLRKLTVLKLWHNSITYIPEHIKKLTSLERLSFSHNKIEVLPSHLFLCNKIRYLDLSYNDIRFIPP
EIGVLQSLQYFSITCNKVESLPDELYFCKKLKTLKIGKNSLSVLSPKIGNLLFLSYLDVKGNHFEILPPE
LGDCRALKRAGLVVEDALFETLPSDVREQMKTE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_032270
ORF Size 2409 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_032270.4
RefSeq Size 7207 bp
RefSeq ORF 2412 bp
Locus ID 84230
UniProt ID Q8TDW0
Cytogenetics 1p22.2
Domains LRR, LRR_TYP, LRR_PS
Protein Families Transmembrane
MW 92.4 kDa
Gene Summary Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes. The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine. Plays a redundant role in the efflux of amino acids, such as aspartate and glutamate, in response to osmotic stress. Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition.[UniProtKB/Swiss-Prot Function]

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