IL12RB1 (NM_005535) Human Tagged ORF Clone

CAT#: RC221974

IL12RB1 (Myc-DDK-tagged)-Human interleukin 12 receptor, beta 1 (IL12RB1), transcript variant 1

Product Images

Circular map for RC221974

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  Western blot validation of overexpression lysate (Cat# LY417240) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221974 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified IL12RB1 protein (Cat# TP321974). The protein was produced from HEK293T cells transfected with IL12RB1 cDNA clone (Cat# RC221974) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IL12RB1
• Synonyms: CD212; IL-12R-BETA1; IL12RB; IMD30
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221974 representing NM_005535
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGCTGGTGACCTGGGTGGTCCCCCTCCTCTTCCTCTTCCTGCTGTCCAGGCAGGGCGCTGCCT
  GCAGAACCAGTGAGTGCTGTTTTCAGGACCCGCCATATCCGGATGCAGACTCAGGCTCGGCCTCGGGCCC
  TAGGGACCTGAGATGCTATCGGATATCCAGTGATCGTTACGAGTGCTCCTGGCAGTATGAGGGTCCCACA
  GCTGGGGTCAGCCACTTCCTGCGGTGTTGCCTTAGCTCCGGGCGCTGCTGCTACTTCGCCGCCGGCTCAG
  CCACCAGGCTGCAGTTCTCCGACCAGGCTGGGGTGTCTGTGCTGTACACTGTCACACTCTGGGTGGAATC
  CTGGGCCAGGAACCAGACAGAGAAGTCTCCTGAGGTCACCCTGCAGCTCTACAACTCAGTTAAATATGAG
  CCTCCTCTGGGAGACATCAAGGTGTCCAAGTTGGCCGGGCAGCTGCGTATGGAGTGGGAGACCCCGGATA
  ACCAGGTTGGTGCTGAGGTGCAGTTCCGGCACCGGACACCCAGCAGCCCATGGAAGTTGGGCGACTGCGG
  ACCTCAGGATGATGATACTGAGTCCTGCCTCTGCCCCCTGGAGATGAATGTGGCCCAGGAATTCCAGCTC
  CGACGACGGCGGCTGGGGAGCCAAGGAAGTTCCTGGAGCAAGTGGAGCAGCCCTGTGTGCGTTCCCCCTG
  AAAACCCCCCACAGCCTCAGGTGAGATTCTCGGTGGAGCAGCTGGGCCAGGATGGGAGGAGGCGGCTGAC
  CCTGAAAGAGCAGCCAACCCAGCTGGAGCTTCCAGAAGGCTGTCAAGGGCTGGCGCCTGGCACGGAGGTC
  ACTTACCGACTACAGCTCCACATGCTGTCCTGCCCGTGTAAGGCCAAGGCCACCAGGACCCTGCACCTGG
  GGAAGATGCCCTATCTCTCGGGTGCTGCCTACAACGTGGCTGTCATCTCCTCGAACCAATTTGGTCCTGG
  CCTGAACCAGACGTGGCACATTCCTGCCGACACCCACACAGAACCAGTGGCTCTGAATATCAGCGTCGGA
  ACCAACGGGACCACCATGTATTGGCCAGCCCGGGCTCAGAGCATGACGTATTGCATTGAATGGCAGCCTG
  TGGGCCAGGACGGGGGCCTTGCCACCTGCAGCCTGACTGCGCCGCAAGACCCGGATCCGGCTGGAATGGC
  AACCTACAGCTGGAGTCGAGAGTCTGGGGCAATGGGGCAGGAAAAGTGTTACTACATTACCATCTTTGCC
  TCTGCGCACCCCGAGAAGCTCACCTTGTGGTCTACGGTCCTGTCCACCTACCACTTTGGGGGCAATGCCT
  CAGCAGCTGGGACACCGCACCACGTCTCGGTGAAGAATCATAGCTTGGACTCTGTGTCTGTGGACTGGGC
  ACCATCCCTGCTGAGCACCTGTCCCGGCGTCCTAAAGGAGTATGTTGTCCGCTGCCGAGATGAAGACAGC
  AAACAGGTGTCAGAGCATCCCGTGCAGCCCACAGAGACCCAAGTTACCCTCAGTGGCCTGCGGGCTGGTG
  TAGCCTACACGGTGCAGGTGCGAGCAGACACAGCGTGGCTGAGGGGTGTCTGGAGCCAGCCCCAGCGCTT
  CAGCATCGAAGTGCAGGTTTCTGATTGGCTCATCTTCTTCGCCTCCCTGGGGAGCTTCCTGAGCATCCTT
  CTCGTGGGCGTCCTTGGCTACCTTGGCCTGAACAGGGCCGCACGGCACCTGTGCCCGCCGCTGCCCACAC
  CCTGTGCCAGCTCCGCCATTGAGTTCCCTGGAGGGAAGGAGACTTGGCAGTGGATCAACCCAGTGGACTT
  CCAGGAAGAGGCATCCCTGCAGGAGGCCCTGGTGGTAGAGATGTCCTGGGACAAAGGCGAGAGGACTGAG
  CCTCTCGAGAAGACAGAGCTACCTGAGGGTGCCCCTGAGCTGGCCCTGGATACAGAGTTGTCCTTGGAGG
  ATGGAGACAGGTGCAAGGCCAAGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221974 representing NM_005535
  Red=Cloning site Green=Tags(s)
  
  MEPLVTWVVPLLFLFLLSRQGAACRTSECCFQDPPYPDADSGSASGPRDLRCYRISSDRYECSWQYEGPT
  AGVSHFLRCCLSSGRCCYFAAGSATRLQFSDQAGVSVLYTVTLWVESWARNQTEKSPEVTLQLYNSVKYE
  PPLGDIKVSKLAGQLRMEWETPDNQVGAEVQFRHRTPSSPWKLGDCGPQDDDTESCLCPLEMNVAQEFQL
  RRRRLGSQGSSWSKWSSPVCVPPENPPQPQVRFSVEQLGQDGRRRLTLKEQPTQLELPEGCQGLAPGTEV
  TYRLQLHMLSCPCKAKATRTLHLGKMPYLSGAAYNVAVISSNQFGPGLNQTWHIPADTHTEPVALNISVG
  TNGTTMYWPARAQSMTYCIEWQPVGQDGGLATCSLTAPQDPDPAGMATYSWSRESGAMGQEKCYYITIFA
  SAHPEKLTLWSTVLSTYHFGGNASAAGTPHHVSVKNHSLDSVSVDWAPSLLSTCPGVLKEYVVRCRDEDS
  KQVSEHPVQPTETQVTLSGLRAGVAYTVQVRADTAWLRGVWSQPQRFSIEVQVSDWLIFFASLGSFLSIL
  LVGVLGYLGLNRAARHLCPPLPTPCASSAIEFPGGKETWQWINPVDFQEEASLQEALVVEMSWDKGERTE
  PLEKTELPEGAPELALDTELSLEDGDRCKAKM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005535
• ORF Size: 1986 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005535.3
• RefSeq Size: 2100 bp
• RefSeq ORF: 1989 bp
• Locus ID: 3594
• UniProt ID: P42701
• Cytogenetics: 19p13.11
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway
• MW: 73.14 kDa
• Gene Summary: The protein encoded by this gene is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukine 12 (IL12) with a low affinity, and is thought to be a part of IL12 receptor complex. This protein forms a disulfide-linked oligomer, which is required for its IL12 binding activity. The coexpression of this and IL12RB2 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. Mutations in this gene impair the development of interleukin-17-producing T lymphocytes and result in increased susceptibility to mycobacterial and Salmonella infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]