IL12RB1 (NM_005535) Human Untagged Clone

CAT#: SC303661

IL12RB1 (untagged)-Human interleukin 12 receptor, beta 1 (IL12RB1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IL12RB1
• Synonyms: CD212; IL-12R-BETA1; IL12RB; IMD30
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005535 edited
  ACCTCGCAGGTGGCAGAGAGGCTCCCCTGGGGCTGTGGGGCTCTACGTGGATCTGATGGA
  GCCGCTGGTGACCTGGGTGGTCCCCCTCCTCTTCCTCTTCCTGCTGTCCAGGCAGGGCGC
  TGCCTGCAGAACCAGTGAGTGCTGTTTTCAGGACCCGCCATATCCGGATGCAGACTCAGG
  CTCGGCCTCGGGCCCTAGGGACCTGAGATGCTATCGGATATCCAGTGATCGTTACGAGTG
  CTCCTGGCAGTATGAGGGTCCCACAGCTGGGGTCAGCCACTTCCTGCGGTGTTGCCTTAG
  CTCCGGGCGCTGCTGCTACTTCGCCGCCGGCTCAGCCACCAGGCTGCAGTTCTCCGACCA
  GGCTGGGGTGTCTGTGCTGTACACTGTCACACTCTGGGTGGAATCCTGGGCCAGGAACCA
  GACAGAGAAGTCTCCTGAGGTCACCCTGCAGCTCTACAACTCAGTTAAATATGAGCCTCC
  TCTGGGAGACATCAAGGTGTCCAAGTTGGCCGGGCAGCTGCGTATGGAGTGGGAGACCCC
  GGATAACCAGGTTGGTGCTGAGGTGCAGTTCCGGCACCGGACACCCAGCAGCCCATGGAA
  GTTGGGCGACTGCGGACCTCAGGATGATGATACTGAGTCCTGCCTCTGCCCCCTGGAGAT
  GAATGTGGCCCAGGAATTCCAGCTCCGACGACGGCGGCTGGGGAGCCAAGGAAGTTCCTG
  GAGCAAGTGGAGCAGCCCTGTGTGCGTTCCCCCTGAAAACCCCCCACAGCCTCAGGTGAG
  ATTCTCGGTGGAGCAGCTGGGCCAGGATGGGAGGAGGCGGCTGACCCTGAAAGAGCAGCC
  AACCCAGCTGGAGCTTCCAGAAGGCTGTCAAGGGCTGGCGCCTGGCACGGAGGTCACTTA
  CCGACTACAGCTCCACATGCTGTCCTGCCCGTGTAAGGCCAAGGCCACCAGGACCCTGCA
  CCTGGGGAAGATGCCCTATCTCTCGGGTGCTGCCTACAACGTGGCTGTCATCTCCTCGAA
  CCAATTTGGTCCTGGCCTGAACCAGACGTGGCACATTCCTGCCGACACCCACACAGAACC
  AGTGGCTCTGAATATCAGCGTCGGAACCAACGGGACCACCATGTATTGGCCAGCCCGGGC
  TCAGAGCATGACGTATTGCATTGAATGGCAGCCTGTGGGCCAGGACGGGGGCCTTGCCAC
  CTGCAGCCTGACTGCGCCGCAAGACCCGGATCCGGCTGGAATGGCAACCTACAGCTGGAG
  TCGAGAGTCTGGGGCAATGGGGCAGGAAAAGTGTTACTACATTACCATCTTTGCCTCTGC
  GCACCCCGAGAAGCTCACCTTGTGGTCTACGGTCCTGTCCACCTACCACTTTGGGGGCAA
  TGCCTCAGCAGCTGGGACACCGCACCACGTCTCGGTGAAGAATCATAGCTTGGACTCTGT
  GTCTGTGGACTGGGCACCATCCCTGCTGAGCACCTGTCCCGGCGTCCTAAAGGAGTATGT
  TGTCCGCTGCCGAGATGAAGACAGCAAACAGGTGTCAGAGCATCCCGTGCAGCCCACAGA
  GACCCAAGTTACCCTCAGTGGCCTGCGGGCTGGTGTAGCCTACACGGTGCAGGTGCGAGC
  AGACACAGCGTGGCTGAGGGGTGTCTGGAGCCAGCCCCAGCGCTTCAGCATCGAAGTGCA
  GGTTTCTGATTGGCTCATCTTCTTCGCCTCCCTGGGGAGCTTCCTGAGCATCCTTCTCGT
  GGGCGTCCTTGGCTACCTTGGCCTGAACAGGGCCGCACGGCACCTGTGCCCGCCGCTGCC
  CACACCCTGTGCCAGCTCCGCCATTGAGTTCCCTGGAGGGAAGGAGACTTGGCAGTGGAT
  CAACCCAGTGGACTTCCAGGAAGAGGCATCCCTGCAGGAGGCCCTGGTGGTAGAGATGTC
  CTGGGACAAAGGCGAGAGGACTGAGCCTCTCGAGAAGACAGAGCTACCTGAGGGTGCCCC
  TGAGCTGGCCCTGGATACAGAGTTGTCCTTGGAGGATGGAGACAGGTGCAAGGCCAAGAT
  GTGATCGTTGAGGCTC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005535 unedited
  GTTCACCATTTGTATACGACTCATATAGGCGGCCGCGNATTCAGATCTGGTACCGAGCTC
  GGATCCACTAGTAACGGCCGCCATGTGCTGGAATTCGCCCTTACCTCGCAGGTGGCAGAG
  AGGCTCCCCTGGGGCTGTGGGGCTCTACGTGGATCTGATGGAGCCGCTGGTGACCTGGGT
  GGTCCCCCTCCTCTTCCTCTTCCTGCTGTCCAGGCAGGGCGCTGCCTGCAGAACCAGTGA
  GTGCTGTTTTCAGGACCCGCCATATCCGGATGCAGACTCAGGCTCGGCCTCGGGCCCTAG
  GGACCTGAGATGCTATCGGATATCCAGTGATCGTTACGAGTGCTCCTGGCAGTATGAGGG
  TCCCACAGCTGGGGTCAGCCACTTCCTGCGGTGTTGCCTTAGCTCCGGGCGCTGCTGCTA
  CTTCGCCGCCGGCTCAGCCACCAGGCTGCAGTTCTCCGACCAGGCTGGGGTGTCTGTGCT
  GTACACTGTCACACTCTGGGTGGAATCCTGGGCCAGGAACCAGACAGAGAAGTCTCCTGA
  GGTCACCCTGCAGCTCTACAACTCAGTTAAATATGAGCCTCCTCTGGGAGACATCAAGGT
  GTCCAAGTTGGCCGGGCAGCTGCGTATGGAGTGGGAGACCCCGGATAACCAGGTTGGTGC
  TNGAGTGCAGTTCCGGCACCGGACACCCAGCAGCCCATGGAAGTTGGGCGACTGCGGACC
  TCANGATGATGATACTGAGTCCTGCCTCTGCCCCCTGGAGATGAATGTGGCCCCAAGAAT
  TCCAGCTCCGACGACGGCGGCTGGGGAGCCAAGGGAAGTCCTGGAGCAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005535 unedited
  CATTGGNGATGGCAACTTCCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGGATGCC
  ACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGATGCATGCTCGAG
  CGGCCGCCAGTGTGATGGATATCTGCAGAATTCGCCCTTGAGCCTCAACGATCACATCTT
  GGCCTTGCACCTGTCTCCATCCTCCAAGGACAACTCTGTATCCAGGGCCAGCTCAGGGGC
  ACCCTCAGGTAGCTCTGTCTTCTCGAGAGGCTCAGTCCTCTCGCCTTTGTCCCAGGACAT
  CTCTACCACCAGGGCCTCCTGCAGGGATGCCTCTTCCTGGAAGTCCACTGGGTTGATCCA
  CTGCCAAGTCTCCTTCCCTCCAGGGAACTCAATGGCGGAGCTGGCACAGGGTGTGGGCAG
  CGGCGGGCACAGGTGCCGTGCGGCCCTGTTCAGGCCAAGGTAGCCAAGGACGCCCACGAG
  AAGGATGCTCAGGAAGCTCCCCAGGGAGGCGAAGAAGATGAGCCAATCAGAAACCTGCAC
  TTCGATGCTGAAGCGCTGGGGCTGGCTCCAGACACCCCTCAGCCACGCTGTGTCTGCTCG
  CACCTGCACCGTGTAGGCTACACCAGCCCCGCAGCCACTGAGGGTAACTTGNGTCTCTGT
  GGGCTGCACGGGATGCTCTGACACCTGTTTGCTGTCTTCATCTCGGCAGCGGACAACATA
  CTCCTTATAGACGCCGGGACAGGTGCTCAACAGGGATGGTGCCCCAGTCCACAGACACAG
  AGTCCAAGCTATGATTCCTTCACCGAGACGTGGTGCGGTGTCCCAGCTGCTGAGGCATTT
  GCCCCCAAAGTGTAAGTGGAACCGGACC
  
• Restriction Sites: Please inquire
• ACCN: NM_005535
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: There are 4 nucleotide differences between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the substitution of 1 amino acid.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005535.1, NP_005526.1
• RefSeq Size: 2100 bp
• RefSeq ORF: 1989 bp
• Locus ID: 3594
• UniProt ID: P42701
• Cytogenetics: 19p13.11
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway
• Gene Summary: The protein encoded by this gene is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukine 12 (IL12) with a low affinity, and is thought to be a part of IL12 receptor complex. This protein forms a disulfide-linked oligomer, which is required for its IL12 binding activity. The coexpression of this and IL12RB2 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. Mutations in this gene impair the development of interleukin-17-producing T lymphocytes and result in increased susceptibility to mycobacterial and Salmonella infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (1) represents the use of an alternate promoter, differs in the 5' UTR and uses a downstream start codon compared to variant 4. The encoded isoform (1, also known as Long) has a shorter N-terminus compared to isoform 4. Sequence Note: The 5' UTR was inferred from partial sequence data.