SOAT 2 (SOAT2) (NM_003578) Human Tagged ORF Clone

CAT#: RC221499

SOAT2 (Myc-DDK-tagged)-Human sterol O-acyltransferase 2 (SOAT2)

Product Images

Circular map for RC221499

• 

  Western blot validation of overexpression lysate (Cat# LY418574) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221499 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SOAT 2
• Synonyms: ACACT2; ACAT2; ARGP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221499 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCAGGCGGGGCCCGTCTGCGTCTGCAGAGGACAGGAGGGCTGGGAGGGGAGCGGGAGCGCCAAC
  CCTGTGGAGATGGAAACACTGAGACGCACAGAGCCCCGGACTTGGTACAATGGACCCGACACATGGAGGC
  TGTGAAGGCACAATTGCTGGAGCAAGCGCAGGGACAACTGAGGGAGCTGCTGGATCGGGCCATGCGGGAG
  GCTATACAATCCTACCCATCACAAGACAAACCTCTGCCCCCACCTCCCCCAGGTTCCTTGAGCAGGACCC
  AGGAGCCATCCCTGGGGAAACAGAAAGTTTTCATCATCCGCAAGTCCCTGCTTGATGAGCTGATGGAGGT
  GCAGCATTTCCGCACCATCTACCACATGTTCATCGCTGGCCTGTGTGTCTTCATCATCAGCACCCTGGCC
  ATCGACTTCATTGATGAGGGCAGGCTGCTGCTGGAGTTTGACCTACTGATCTTCAGCTTCGGACAGCTGC
  CATTGGCGCTGGTGACCTGGGTGCCCATGTTTCTGTCCACCCTGTTGGCGCCGTACCAGGCCCTACGGCT
  GTGGGCCAGGGGCACCTGGACGCAGGCGACGGGCCTGGGCTGTGCGCTTTTAGCCGCCCACGCCGTGGTG
  CTCTGCGCGCTGCCGGTCCACGTGGCCGTGGAGCATCAGCTCCCGCCGGCCTCCCGTTGTGTCCTGGTCT
  TCGAGCAGGTTAGGTTCCTGATGAAAAGCTACTCCTTCCTGAGAGAGGCTGTGCCTGGGATCCTTCGTGC
  CAGACGAGGTGAGGGGATCCAGGCCCCCAGTTTCTCCAGCTACCTCTACTTCCTCTTCTGCCCAACACTC
  ATCTACAGGGAGACTTACCCTAGGACGCCCTATGTCAGGTGGAATTATGTGGCCAAGAACTTTGCCCAGG
  CCCTGGGATGTGTGCTCTATGCCTGCTTCATCCTGGGCCGCCTCTGTGTTCCTGTCTTTGCCAACATGAG
  CCGAGAGCCCTTCAGCACCCGTGCCCTGGTGCTCTCTATCCTGCATGCCACGTTGCCAGGCATCTTCATG
  CTGCTGCTCATCTTCTTTGCCTTCCTCCATTGCTGGCTCAACGCCTTTGCCGAGATGCTACGATTTGGAG
  ACAGGATGTTCTACCGGGACTGGTGGAACTCAACGTCCTTCTCCAACTACTACCGCACTTGGAACGTGGT
  GGTCCATGACTGGCTGTACAGCTACGTGTATCAGGATGGGCTGCGGCTCCTTGGTGCCCGGGCCCGAGGG
  GTAGCCATGCTGGGTGTGTTCCTGGTCTCCGCAGTGGCCCATGAGTATATCTTCTGCTTCGTCCTGGGGT
  TCTTCTATCCCGTCATGCTGATACTCTTCCTTGTCATTGGAGGAATGTTGAACTTCATGATGCATGACCA
  GCGCACCGGCCCGGCATGGAACGTGCTGATGTGGACCATGCTGTTTCTAGGCCAGGGAATCCAGGTCAGC
  CTGTACTGCCAGGAGTGGTACGCACGGCGGCACTGCCCCTTACCCCAGGCAACTTTCTGGGGGCTGGTGA
  CACCTCGATCTTGGTCCTGCCATACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221499 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPGGARLRLQRTGGLGGERERQPCGDGNTETHRAPDLVQWTRHMEAVKAQLLEQAQGQLRELLDRAMRE
  AIQSYPSQDKPLPPPPPGSLSRTQEPSLGKQKVFIIRKSLLDELMEVQHFRTIYHMFIAGLCVFIISTLA
  IDFIDEGRLLLEFDLLIFSFGQLPLALVTWVPMFLSTLLAPYQALRLWARGTWTQATGLGCALLAAHAVV
  LCALPVHVAVEHQLPPASRCVLVFEQVRFLMKSYSFLREAVPGILRARRGEGIQAPSFSSYLYFLFCPTL
  IYRETYPRTPYVRWNYVAKNFAQALGCVLYACFILGRLCVPVFANMSREPFSTRALVLSILHATLPGIFM
  LLLIFFAFLHCWLNAFAEMLRFGDRMFYRDWWNSTSFSNYYRTWNVVVHDWLYSYVYQDGLRLLGARARG
  VAMLGVFLVSAVAHEYIFCFVLGFFYPVMLILFLVIGGMLNFMMHDQRTGPAWNVLMWTMLFLGQGIQVS
  LYCQEWYARRHCPLPQATFWGLVTPRSWSCHT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003578
• ORF Size: 1566 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003578.4
• RefSeq Size: 2073 bp
• RefSeq ORF: 1569 bp
• Locus ID: 8435
• UniProt ID: O75908
• Cytogenetics: 12q13.13
• Protein Families: Transmembrane
• Protein Pathways: Steroid biosynthesis
• MW: 59.8 kDa
• Gene Summary: Summary:This gene is a member of a small family of acyl coenzyme A:cholesterol acyltransferases. The gene encodes a membrane-bound enzyme localized in the endoplasmic reticulum that produces intracellular cholesterol esters from long-chain fatty acyl CoA and cholesterol. The cholesterol esters are then stored as cytoplasmic lipid droplets inside the cell. The enzyme is implicated in cholesterol absorption in the intestine and in the assembly and secretion of apolipoprotein B-containing lipoproteins such as very low density lipoprotein (VLDL). Several alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2008]