ZBTB7C (NM_001039360) Human Tagged ORF Clone

CAT#: RC221474

ZBTB7C (Myc-DDK-tagged)-Human zinc finger and BTB domain containing 7C (ZBTB7C)

Product Images

Circular map for RC221474

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  Western blot validation of overexpression lysate (Cat# LY422035) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221474 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ZBTB7C protein (Cat# TP321474). The protein was produced from HEK293T cells transfected with ZBTB7C cDNA clone (Cat# RC221474) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZBTB7C
• Synonyms: APM-1; APM1; ZBTB36; ZNF857C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221474 representing NM_001039360
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAATGACATTGATGAGCTCATTGGCATTCCCTTCCCCAACCACAGCAGTGAGGTCCTGTGCAGCC
  TCAATGAGCAACGGCACGATGGCCTGCTGTGTGACGTGCTCCTGGTGGTGCAGGAGCAGGAGTATCGGAC
  CCACCGCTCCGTCCTGGCTGCCTGCAGCAAGTACTTCAAGAAGCTTTTCACAGCCGGCACCCTAGCCAGC
  CAGCCCTACGTCTATGAGATCGACTTTGTCCAGCCTGAGGCTCTGGCTGCTATCCTGGAGTTCGCCTACA
  CCTCCACGCTCACCATCACCGCTGGCAATGTCAAGCACATCCTCAACGCAGCCAGGATGCTGGAGATCCA
  GTGCATCGTGAACGTGTGCCTGGAGATCATGGAGCCTGGGGGGGACGGGGGGGAGGAGGATGACAAGGAG
  GACGATGACGACGACGAAGATGATGATGATGAGGAGGACGAAGAGGAGGAGGAGGAAGAGGAGGAGGATG
  ACGATGATGACACGGAGGACTTTGCTGACCAAGAAAACTTGCCTGACCCCCAGGACATCAGCTGCCACCA
  AAGCCCTTCCAAGACAGACCATCTCACAGAGAAGGCCTATTCAGACACCCCCAGGGACTTCCCTGACTCC
  TTCCAGGCTGGCAGTCCTGGCCATCTGGGGGTGATCCGGGACTTCTCCATCGAATCTCTGCTAAGGGAGA
  ACCTGTACCCCAAGGCCAACATCCCCGACAGGAGACCCTCCTTGTCTCCATTCGCCCCGGACTTCTTTCC
  ACACCTCTGGCCAGGGGACTTCGGTGCCTTTGCCCAGCTGCCTGAGCAGCCCATGGACAGTGGGCCACTG
  GATCTGGTCATCAAGAATCGGAAGATCAAGGAGGAGGAGAAGGAGGAGCTGCCCCCACCCCCACCGCCAC
  CCTTCCCTAATGACTTCTTCAAGGACATGTTCCCTGACCTGCCGGGGGGGCCTCTGGGACCCATCAAGGC
  GGAGAACGACTACGGTGCCTATCTCAACTTCCTGAGTGCCACCCACCTGGGAGGCCTCTTCCCACCCTGG
  CCCCTGGTAGAAGAGCGCAAGCTGAAGCCCAAGGCCTCTCAGCAGTGCCCCATCTGCCACAAAGTCATCA
  TGGGGGCCGGGAAGCTGCCGCGGCACATGAGGACCCATACCGGGGAGAAGCCATACATGTGCACCATCTG
  CGAGGTCCGCTTCACCAGGCAGGACAAGCTGAAAATCCACATGCGGAAGCACACAGGGGAGCGGCCCTAC
  CTGTGCATCCACTGCAACGCCAAGTTCGTGCACAACTACGACCTCAAGAACCACATGCGCATCCACACGG
  GCGTGCGGCCCTACCAGTGCGAGTTCTGCTACAAGAGCTTCACGCGCTCTGACCACCTGCACCGCCACAT
  CAAGCGCCAGAGCTGCCGCATGGCACGGCCCCGACGCGGCCGCAAGCCTGCTGCGTGGAGGGCCGCCAGC
  CTGCTCTTCGGGCCCGGCGGCCCGGCCCCCGACAAGGCGGCCTTCGTGATGCCCCCTGCGCTGGGCGAGG
  TGGGCGGCCACCTGGGCGGCGCAGCTGTGTGCCTCCCGGGCCCCAGCCCCGCCAAGCACTTCCTGGCAGC
  GCCCAAGGGCGCCCTGAGCCTGCAAGAGCTGGAGCGGCAGTTCGAGGAGACACAGATGAAGCTGTTCGGG
  CGCGCGCAGCTGGAGGCTGAGAGGAACGCGGGGGGCCTCCTGGCCTTCGCGCTGGCCGAGAACGTGGCGG
  CGGCGCGGCCCTACTTCCCGCTGCCCGACCCTTGGGCCGCCGGCCTGGCCGGCCTCCCTGGGCTCGCCGG
  CCTCAACCACGTGGCCTCCATGTCCGAAGCCAACAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221474 representing NM_001039360
  Red=Cloning site Green=Tags(s)
  
  MANDIDELIGIPFPNHSSEVLCSLNEQRHDGLLCDVLLVVQEQEYRTHRSVLAACSKYFKKLFTAGTLAS
  QPYVYEIDFVQPEALAAILEFAYTSTLTITAGNVKHILNAARMLEIQCIVNVCLEIMEPGGDGGEEDDKE
  DDDDDEDDDDEEDEEEEEEEEEDDDDDTEDFADQENLPDPQDISCHQSPSKTDHLTEKAYSDTPRDFPDS
  FQAGSPGHLGVIRDFSIESLLRENLYPKANIPDRRPSLSPFAPDFFPHLWPGDFGAFAQLPEQPMDSGPL
  DLVIKNRKIKEEEKEELPPPPPPPFPNDFFKDMFPDLPGGPLGPIKAENDYGAYLNFLSATHLGGLFPPW
  PLVEERKLKPKASQQCPICHKVIMGAGKLPRHMRTHTGEKPYMCTICEVRFTRQDKLKIHMRKHTGERPY
  LCIHCNAKFVHNYDLKNHMRIHTGVRPYQCEFCYKSFTRSDHLHRHIKRQSCRMARPRRGRKPAAWRAAS
  LLFGPGGPAPDKAAFVMPPALGEVGGHLGGAAVCLPGPSPAKHFLAAPKGALSLQELERQFEETQMKLFG
  RAQLEAERNAGGLLAFALAENVAAARPYFPLPDPWAAGLAGLPGLAGLNHVASMSEANN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039360
• ORF Size: 1857 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039360.3
• RefSeq Size: 3774 bp
• RefSeq ORF: 1860 bp
• Locus ID: 201501
• UniProt ID: A1YPR0
• Cytogenetics: 18q21.1
• MW: 68.8 kDa
• Gene Summary: May be a tumor suppressor gene.[UniProtKB/Swiss-Prot Function]