FBXW12 (NM_207102) Human Tagged ORF Clone

CAT#: RC220761

FBXW12 (Myc-DDK-tagged)-Human F-box and WD repeat domain containing 12 (FBXW12), transcript variant 1

Product Images

Circular map for RC220761

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  Western blot validation of overexpression lysate (Cat# LY404111) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220761 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FBXW12
• Synonyms: FBW12; FBXO12; FBXO35
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220761 representing NM_207102
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATCCGATTGCCTGACTTAGCTTTGAAGCGAATCTTCTCTTTCCTGGACCTGTTCGGCTTGCTGC
  AGGTTTCCCAGGTGAACAAGCATTGGAATAGGATTGCAGACAGTGATTACCTGTGGAGGTCACTATCTCT
  GCAGAGATGGGACTGCAGCAACTTCACCAATCAACACCTGGGCACACACACATGGAAGCAATTTTTCCTG
  CATCAAAGAAGGAAGGAGCTTCGGTTGGCATTGGCACAGCCGCATAACTTTATCTACAAAGTAACTAAGA
  ACATCGCATTTGAGACGGAGTTGGCTTATCTCTCAGGAAATAGACTTACAGTGGATGAACAGGAGAAATC
  AATCATTTGTAGTGTATCTCCAAAGCAAGAGCTTTGTGCCTGGGATGTGCAAGAGGGTACCATGATCTGG
  TCAAGCCCAGTCCAGGAGTTCCATTTCTCAAATCTGGTAACCCTCCCTCAGATGCATCTCGCCATCACTA
  TGGATCGGAAAAAAACTATCAAAGTGTGGAACTGTCAGGACAGGGACGCTCTGGCTGTTCTCCCCATGCC
  ACAGCCCTGTTATTGCATGGAAGCCTATCTTACAAAGGATGGCCCATTCCTGATGGTTGGCGATGCTGCA
  GGTGACATCTACACATTTACACTGCCTGGGTTAAGAGATGTTTCTAAAGTTACTGCATTTCAATATGGTA
  TTGTACTTCTACACTGCTCTCCTGACAAGAAATGGGTATTTGCATGTGGGACATACAGTCGTACCTTGCC
  ACAGGTATTCCTCACAGAGTCCTTACTGAGACCATCAGAAGGCAGTGATCCTCTGTCTACCTTTCTCCCA
  CATAAATTATGTGCCAGCGCCTGCTGGACCCCAAAGGTGAAAAACAGGATAACACTGATGTCCCAAAGTA
  GCACTGGAAAAAAGACAGAATTTATCACCTTTGATCTACCAACCAAGAAGACTGGAGGCCAAACAGTCAT
  CCAAGCATATGAGATCGCAAGTTTCCAGGTGGCAGCTCATCTGAAGTGCCCTATCTGGATGGGAGCCAGT
  GATGGATATATGATTGTCTTTACCAGTGGACCATACTTGTTACTCTTCAGCATCACTGGCTTCCTGCTGC
  AACGATTTGAGGACCATCAGGCAGCCATCAACAACTTCTGGGTGGATCCTTGCTATGTGCTCACCACATC
  CGAGAACTCTGTGCACGTGTACATGTGGGAAGAAGGAGGCCGCCATCCATACCTCAGGAGCTGCTGTCAC
  CTGGAAAACACGTGGCATGATCACACAACAGACAGCTGCATCTCCAGTGTGATGTGTGATAATGCAAGCA
  TAGTACTTAGGGTGAGGAAAGTAAGTGACTCCAGCATTCTGGTGATGTATTCTTTGAATACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220761 representing NM_207102
  Red=Cloning site Green=Tags(s)
  
  MEIRLPDLALKRIFSFLDLFGLLQVSQVNKHWNRIADSDYLWRSLSLQRWDCSNFTNQHLGTHTWKQFFL
  HQRRKELRLALAQPHNFIYKVTKNIAFETELAYLSGNRLTVDEQEKSIICSVSPKQELCAWDVQEGTMIW
  SSPVQEFHFSNLVTLPQMHLAITMDRKKTIKVWNCQDRDALAVLPMPQPCYCMEAYLTKDGPFLMVGDAA
  GDIYTFTLPGLRDVSKVTAFQYGIVLLHCSPDKKWVFACGTYSRTLPQVFLTESLLRPSEGSDPLSTFLP
  HKLCASACWTPKVKNRITLMSQSSTGKKTEFITFDLPTKKTGGQTVIQAYEIASFQVAAHLKCPIWMGAS
  DGYMIVFTSGPYLLLFSITGFLLQRFEDHQAAINNFWVDPCYVLTTSENSVHVYMWEEGGRHPYLRSCCH
  LENTWHDHTTDSCISSVMCDNASIVLRVRKVSDSSILVMYSLNT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207102
• ORF Size: 1392 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207102.1, NP_996985.1
• RefSeq Size: 1647 bp
• RefSeq ORF: 1395 bp
• Locus ID: 285231
• UniProt ID: Q6X9E4
• Cytogenetics: 3p21.31
• Protein Families: Druggable Genome
• MW: 52.9 kDa
• Gene Summary: Members of the F-box protein family, such as FBXW12, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603034), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]