GIT1 (NM_014030) Human Tagged ORF Clone

CAT#: RC220153

GIT1 (Myc-DDK-tagged)-Human G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant 2

Product Images

Circular map for RC220153

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  Western blot validation of overexpression lysate (Cat# LY415510) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220153 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GIT1 protein (Cat# TP320153). The protein was produced from HEK293T cells transfected with GIT1 cDNA clone (Cat# RC220153) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GIT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220153 representing NM_014030
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCGAAAGGGGCCGCGAGCGGAGGTGTGTGCGGACTGCAGCGCCCCGGACCCTGGCTGGGCATCCA
  TCAGCAGGGGTGTGCTGGTGTGTGACGAGTGCTGCAGCGTGCACCGGAGCCTGGGACGCCACATCTCCAT
  TGTCAAGCACCTTCGCCACAGCGCCTGGCCTCCCACGCTGCTGCAGATGGTGCACACGCTTGCCAGCAAC
  GGGGCCAACTCCATCTGGGAGCACTCCCTGCTGGACCCCGCACAAGTGCAGAGCGGCCGGCGTAAAGCCA
  ACCCCCAAGACAAAGTCCACCCCATCAAGTCAGAGTTCATCAGGGCCAAGTACCAGATGCTGGCATTTGT
  GCACAAGCTTCCCTGCCGGGACGATGATGGAGTCACCGCCAAAGACCTCAGCAAGCAACTACACTCGAGC
  GTGCGGACAGGCAACCTGGAGACATGTCTGCGCCTGCTCTCCCTGGGTGCCCAGGCCAACTTCTTCCACC
  CAGAGAAGGGCACCACACCTCTGCACGTGGCTGCCAAGGCAGGACAGACACTGCAGGCCGAGCTGCTTGT
  AGTGTATGGGGCTGACCCTGGCTCCCCTGATGTTAATGGCCGCACACCCATTGACTATGCCAGGCAGGCG
  GGGCACCATGAGCTGGCGGAAAGGCTGGTTGAGTGCCAATATGAGCTCACTGACCGGCTGGCCTTCTACC
  TCTGTGGACGCAAGCCGGATCACAAGAATGGGCATTACATCATCCCACAGATGGCTGACAGCCTTGACTT
  ATCCGAATTGGCCAAAGCTGCTAAGAAGAAGCTGCAGGCGCTCAGCAACCGGCTTTTTGAGGAACTCGCC
  ATGGACGTGTATGACGAGGTGGATCGAAGAGAAAATGATGCAGTGTGGCTGGCTACCCAAAACCACAGCA
  CTCTGGTGACAGAGCGCAGTGCCGTGCCCTTCCTGCCTGTTAACCCGGAATACTCAGCCACGCGGAATCA
  GGGGCGACAAAAGCTGGCCCGCTTTAATGCCCGAGAGTTTGCCACCTTGATCATCGACATTCTCAGTGAG
  GCCAAGCGGAGACAGCAGGGCAAGAGCCTGAGCAGCCCCACAGACAACCTCGAGCTGTCTCTGCGGAGCC
  AGAGTGACCTCGACGACCAACACGACTACGACAGCGTGGCCTCTGACGAGGACACAGACCAGGAGCCCCT
  GCGCAGCACCGGCGCCACTCGGAGCAACCGGGCCCGGAGCATGGACTCCTCGGACTTGTCTGACGGGGCT
  GTGACGCTGCAGGAGTACCTGGAGCTGAAGAAGGCCCTGGCTACATCGGAGGCAAAGGTGCAGCAGCTCA
  TGAAGGTCAACAGTAGCCTGAGCGACGAGCTCCGGAGGCTGCAGCGAGAGATCCACAAGCTGCAGGCGGA
  GAACCTGCAGCTCCGGCAGCCTCCAGGGCCGGTGCCCACACCTCCACTCCCCAGTGAACGGGCGGAACAC
  ACACCCATGGCGCCAGGCGGGAGCACACACCGCAGGGATCGCCAGGCCTTTTCCATGTATGAACCTGGCT
  CTGCCCTGAAGCCCTTTGGGGGCCCCCCTGGGGACGAGCTCACTACGCGGCTGCAGCCTTTCCACAGCAC
  TGAGCTAGAGGACGACGCCATCTATTCAGTGCACGTCCCTGCTGGCCTTTACCGGATCCGGAAAGGGGTG
  TCTGCCTCAGCTGTGCCCTTCACTCCCTCCTCCCCGCTGCTGTCCTGCTCCCAGGAGGGAAGCCGCCACA
  CGAGCAAGCTTTCCCGCCACGGCAGTGGAGCCGACAGTGACTATGAGAACACGCAAAGTGGGGACCCACT
  GCTGGGGCTGGAAGGGAAGAGGTTTCTAGAGCTGGGCAAAGAGGAAGACTTCCACCCAGAGCTGGAAAGC
  CTGGATGGAGACCTAGATCCTGGGCTTCCCAGCACAGAGGATGTCATCTTGAAGACAGAGCAGGTCACCA
  AGAACATTCAGGAACTGTTGCGGGCAGCCCAGGAGTTCAAGCATGACAGCTTCGTGCCCTGCTCAGAGAA
  GATCCATTTGGCTGTGACCGAGATGGCCTCCCTCTTCCCAAAGAGGCCAGCCCTGGAGCCAGTGCGGAGC
  TCACTGCGGCTGCTCAACGCCAGCGCCTACCGGCTGCAGAGTGAGTGCCGGAAGACAGTGCCCCCAGAGC
  CCGGCGCCCCAGTGGACTTCCAGCTGCTGACTCAGCAGGTGATCCAGTGCGCCTATGACATCGCCAAGGC
  TGCCAAGCAGCTGGTCACCATCACCACCCGAGAGAAGAAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220153 representing NM_014030
  Red=Cloning site Green=Tags(s)
  
  MSRKGPRAEVCADCSAPDPGWASISRGVLVCDECCSVHRSLGRHISIVKHLRHSAWPPTLLQMVHTLASN
  GANSIWEHSLLDPAQVQSGRRKANPQDKVHPIKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSS
  VRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQTLQAELLVVYGADPGSPDVNGRTPIDYARQA
  GHHELAERLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADSLDLSELAKAAKKKLQALSNRLFEELA
  MDVYDEVDRRENDAVWLATQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSE
  AKRRQQGKSLSSPTDNLELSLRSQSDLDDQHDYDSVASDEDTDQEPLRSTGATRSNRARSMDSSDLSDGA
  VTLQEYLELKKALATSEAKVQQLMKVNSSLSDELRRLQREIHKLQAENLQLRQPPGPVPTPPLPSERAEH
  TPMAPGGSTHRRDRQAFSMYEPGSALKPFGGPPGDELTTRLQPFHSTELEDDAIYSVHVPAGLYRIRKGV
  SASAVPFTPSSPLLSCSQEGSRHTSKLSRHGSGADSDYENTQSGDPLLGLEGKRFLELGKEEDFHPELES
  LDGDLDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPKRPALEPVRS
  SLRLLNASAYRLQSECRKTVPPEPGAPVDFQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014030
• ORF Size: 2283 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014030.4
• RefSeq Size: 3758 bp
• RefSeq ORF: 2286 bp
• Locus ID: 28964
• UniProt ID: Q9Y2X7
• Cytogenetics: 17q11.2
• Domains: ArfGap, ANK, GIT
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, Regulation of actin cytoskeleton
• MW: 84.2 kDa
• Gene Summary: GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve ENTR1 and PTPN13 (By similarity).[UniProtKB/Swiss-Prot Function]