GIT1 (NM_014030) Human Untagged Clone

CAT#: SC310126

GIT1 (untagged)-Human G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GIT1
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014030, the custom clone sequence may differ by one or more nucleotides
  ATGTCCCGAAAGGGGCCGCGAGCGGAGGTGTGTGCGGACTGCAGCGCCCCGGACCCTGGC
  TGGGCATCCATCAGCAGGGGTGTGCTGGTGTGTGACGAGTGCTGCAGCGTGCACCGGAGC
  CTGGGACGCCACATCTCCATTGTCAAGCACCTTCGCCACAGCGCCTGGCCTCCCACGCTG
  CTGCAGATGGTGCACACGCTTGCCAGCAACGGGGCCAACTCCATCTGGGAGCACTCCCTG
  CTGGACCCCGCACAAGTGCAGAGCGGCCGGCGTAAAGCCAACCCCCAAGACAAAGTCCAC
  CCCATCAAGTCAGAGTTCATCAGGGCCAAGTACCAGATGCTGGCATTTGTGCACAAGCTT
  CCCTGCCGGGACGATGATGGAGTCACCGCCAAAGACCTCAGCAAGCAACTACACTCGAGC
  GTGCGGACAGGCAACCTGGAGACATGTCTGCGCCTGCTCTCCCTGGGTGCCCAGGCCAAC
  TTCTTCCACCCAGAGAAGGGCACCACACCTCTGCACGTGGCTGCCAAGGCAGGACAGACA
  CTGCAGGCCGAGCTGCTTGTAGTGTATGGGGCTGACCCTGGCTCCCCTGATGTTAATGGC
  CGCACACCCATTGACTATGCCAGGCAGGCGGGGCACCATGAGCTGGCGGAAAGGCTGGTT
  GAGTGCCAATATGAGCTCACTGACCGGCTGGCCTTCTACCTCTGTGGACGCAAGCCGGAT
  CACAAGAATGGGCATTACATCATCCCACAGATGGCTGACAGCCTTGACTTATCCGAATTG
  GCCAAAGCTGCTAAGAAGAAGCTGCAGGCGCTCAGCAACCGGCTTTTTGAGGAACTCGCC
  ATGGACGTGTATGACGAGGTGGATCGAAGAGAAAATGATGCAGTGTGGCTGGCTACCCAA
  AACCACAGCACTCTGGTGACAGAGCGCAGTGCCGTGCCCTTCCTGCCTGTTAACCCGGAA
  TACTCAGCCACGCGGAATCAGGGGCGACAAAAGCTGGCCCGCTTTAATGCCCGAGAGTTT
  GCCACCTTGATCATCGACATTCTCAGTGAGGCCAAGCGGAGACAGCAGGGCAAGAGCCTG
  AGCAGCCCCACAGACAACCTCGAGCTGTCTCTGCGGAGCCAGAGTGACCTCGACGACCAA
  CACGACTACGACAGCGTGGCCTCTGACGAGGACACAGACCAGGAGCCCCTGCGCAGCACC
  GGCGCCACTCGGAGCAACCGGGCCCGGAGCATGGACTCCTCGGACTTGTCTGACGGGGCT
  GTGACGCTGCAGGAGTACCTGGAGCTGAAGAAGGCCCTGGCTACATCGGAGGCAAAGGTG
  CAGCAGCTCATGAAGGTCAACAGTAGCCTGAGCGACGAGCTCCGGAGGCTGCAGCGAGAG
  ATCCACAAGCTGCAGGCGGAGAACCTGCAGCTCCGGCAGCCTCCAGGGCCGGTGCCCACA
  CCTCCACTCCCCAGTGAACGGGCGGAACACACACCCATGGCGCCAGGCGGGAGCACACAC
  CGCAGGGATCGCCAGGCCTTTTCCATGTATGAACCTGGCTCTGCCCTGAAGCCCTTTGGG
  GGCCCCCCTGGGGACGAGCTCACTACGCGGCTGCAGCCTTTCCACAGCACTGAGCTAGAG
  GACGACGCCATCTATTCAGTGCACGTCCCTGCTGGCCTTTACCGGATCCGGAAAGGGGTG
  TCTGCCTCAGCTGTGCCCTTCACTCCCTCCTCCCCGCTGCTGTCCTGCTCCCAGGAGGGA
  AGCCGCCACACGAGCAAGCTTTCCCGCCACGGCAGTGGAGCCGACAGTGACTATGAGAAC
  ACGCAAAGTGGGGACCCACTGCTGGGGCTGGAAGGGAAGAGGTTTCTAGAGCTGGGCAAA
  GAGGAAGACTTCCACCCAGAGCTGGAAAGCCTGGATGGAGACCTAGATCCTGGGCTTCCC
  AGCACAGAGGATGTCATCTTGAAGACAGAGCAGGTCACCAAGAACATTCAGGAACTGTTG
  CGGGCAGCCCAGGAGTTCAAGCATGACAGCTTCGTGCCCTGCTCAGAGAAGATCCATTTG
  GCTGTGACCGAGATGGCCTCCCTCTTCCCAAAGAGGCCAGCCCTGGAGCCAGTGCGGAGC
  TCACTGCGGCTGCTCAACGCCAGCGCCTACCGGCTGCAGAGTGAGTGCCGGAAGACAGTG
  CCCCCAGAGCCCGGCGCCCCAGTGGACTTCCAGCTGCTGACTCAGCAGGTGATCCAGTGC
  GCCTATGACATCGCCAAGGCTGCCAAGCAGCTGGTCACCATCACCACCCGAGAGAAGAAG
  CAGTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_014030
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014030.2, NP_054749.2
• RefSeq Size: 3758 bp
• RefSeq ORF: 2286 bp
• Locus ID: 28964
• UniProt ID: Q9Y2X7
• Cytogenetics: 17q11.2
• Domains: ArfGap, ANK, GIT
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, Regulation of actin cytoskeleton
• Gene Summary: GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve ENTR1 and PTPN13 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 2).