TMEM168 (NM_022484) Human Tagged ORF Clone

CAT#: RC220027

TMEM168 (Myc-DDK-tagged)-Human transmembrane protein 168 (TMEM168)

Product Images

Circular map for RC220027

• 

  Western blot validation of overexpression lysate (Cat# LY411651) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220027 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMEM168
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220027 representing NM_022484
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTAAATCACTGCGTTATTGCTTTAGTCATTGTCTCTATTTAGCAATGACAAGACTGGAAGAAGTAA
  ATAGAGAAGTGAACATGCATTCTTCAGTGCGGTATCTTGGCTATTTAGCCAGAATCAATTTATTGGTTGC
  TATATGCTTAGGTCTATACGTAAGATGGGAAAAAACAGCAAATTCCTTAATTTTGGTAATTTTTATTCTT
  GGTCTTTTTGTTCTTGGAATCGCCAGCATACTCTATTACTATTTTTCAATGGAAGCAGCAAGTTTAAGTC
  TCTCCAATCTTTGGTTTGGATTCTTGCTTGGCCTCCTATGTTTTCTTGATAATTCATCCTTTAAAAATGA
  TGTAAAAGAAGAATCAACCAAATATTTGCTTCTAACATCCATAGTGTTAAGGATATTGTGCTCTCTGGTG
  GAGAGAATTTCTGGTTATGTCCGTCATCGGCCCACTTTACTAACCACAGTTGAATTTCTGGAGCTTGTTG
  GATTTGCCATTGCCAGCACAACTATGTTGGTGGAGAAGTCTCTGAGTGTCATTTTGCTTGTTGTAGCTCT
  GGCTATGCTGATTATTGATCTGAGAATGAAATCTTTCTTAGCTATTCCAAACTTAGTTATTTTTGCAGTT
  TTGTTATTTTTTTCCTCATTGGAAACTCCCAAAAATCCGATTGCTTTTGCGTGTTTTTTTATTTGCCTGA
  TAACTGATCCTTTCCTTGACATTTATTTTAGTGGACTTTCAGTAACTGAAAGATGGAAACCCTTTTTGTA
  CCGTGGAAGAATTTGCAGAAGACTTTCAGTCGTTTTTGCTGGAATGATTGAGCTTACATTTTTTATTCTT
  TCCGCATTCAAACTTAGAGACACTCACCTCTGGTATTTTGTAATACCTGGCTTTTCCATTTTTGGAATTT
  TCTGGATGATTTGTCATATTATTTTTCTTTTAACTCTTTGGGGATTCCATACCAAATTAAATGACTGCCA
  TAAAGTATATTTTACTCACAGGACAGATTACAATAGCCTTGATAGAATCATGGCATCCAAAGGGATGCGC
  CATTTTTGCTTGATTTCAGAGCAGTTGGTGTTCTTTAGTCTTCTTGCAACAGCGATTTTGGGAGCAGTTT
  CCTGGCAGCCAACAAATGGAATTTTCTTGAGCATGTTTCTAATCGTTTTGCCATTGGAATCCATGGCTCA
  TGGGCTCTTCCATGAATTGGGTAACTGTTTAGGAGGAACATCTGTTGGATATGCTATTGTGATTCCCACC
  AACTTCTGCAGTCCTGATGGTCAGCCAACACTGCTTCCCCCAGAACATGTACAGGAGTTAAATTTGAGGT
  CTACTGGCATGCTCAATGCTATCCAAAGATTTTTTGCATATCATATGATTGAGACCTATGGATGTGACTA
  TTCCACAAGTGGACTGTCATTTGATACTCTGCATTCCAAACTAAAAGCTTTCCTCGAACTTCGGACAGTG
  GATGGACCCAGACATGATACGTATATTTTGTATTACAGTGGGCACACCCATGGTACAGGAGAGTGGGCTC
  TAGCAGGTGGAGATACACTACGCCTTGACACACTTATAGAATGGTGGAGAGAAAAGAATGGTTCCTTTTG
  TTCCCGGCTTATTATCGTATTAGACAGCGAAAATTCAACCCCTTGGGTGAAAGAAGTGAGGAAAATTAAT
  GACCAGTATATTGCAGTGCAAGGAGCAGAGTTGATAAAAACAGTAGATATTGAAGAAGCTGACCCGCCAC
  AGCTAGGTGACTTTACAAAAGACTGGGTAGAATATAACTGCAACTCCAGTAATAACATCTGCTGGACTGA
  AAAGGGACGCACAGTGAAAGCAGTATATGGTGTGTCAAAACGGTGGAGTGACTACACTCTGCATTTGCCA
  ACGGGAAGCGATGTGGCCAAGCACTGGATGTTACACTTTCCTCGTATTACATATCCCCTAGTGCATTTGG
  CAAATTGGTTATGCGGTCTGAACCTTTTTTGGATCTGCAAAACTTGTTTTAGGTGCTTGAAAAGATTAAA
  AATGAGTTGGTTTCTTCCTACTGTGCTGGACACAGGACAAGGCTTCAAACTTGTCAAATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220027 representing NM_022484
  Red=Cloning site Green=Tags(s)
  
  MCKSLRYCFSHCLYLAMTRLEEVNREVNMHSSVRYLGYLARINLLVAICLGLYVRWEKTANSLILVIFIL
  GLFVLGIASILYYYFSMEAASLSLSNLWFGFLLGLLCFLDNSSFKNDVKEESTKYLLLTSIVLRILCSLV
  ERISGYVRHRPTLLTTVEFLELVGFAIASTTMLVEKSLSVILLVVALAMLIIDLRMKSFLAIPNLVIFAV
  LLFFSSLETPKNPIAFACFFICLITDPFLDIYFSGLSVTERWKPFLYRGRICRRLSVVFAGMIELTFFIL
  SAFKLRDTHLWYFVIPGFSIFGIFWMICHIIFLLTLWGFHTKLNDCHKVYFTHRTDYNSLDRIMASKGMR
  HFCLISEQLVFFSLLATAILGAVSWQPTNGIFLSMFLIVLPLESMAHGLFHELGNCLGGTSVGYAIVIPT
  NFCSPDGQPTLLPPEHVQELNLRSTGMLNAIQRFFAYHMIETYGCDYSTSGLSFDTLHSKLKAFLELRTV
  DGPRHDTYILYYSGHTHGTGEWALAGGDTLRLDTLIEWWREKNGSFCSRLIIVLDSENSTPWVKEVRKIN
  DQYIAVQGAELIKTVDIEEADPPQLGDFTKDWVEYNCNSSNNICWTEKGRTVKAVYGVSKRWSDYTLHLP
  TGSDVAKHWMLHFPRITYPLVHLANWLCGLNLFWICKTCFRCLKRLKMSWFLPTVLDTGQGFKLVKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022484
• ORF Size: 2091 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022484.6
• RefSeq Size: 3951 bp
• RefSeq ORF: 2094 bp
• Locus ID: 64418
• UniProt ID: Q9H0V1
• Cytogenetics: 7q31.1
• Protein Families: Transmembrane
• MW: 79.6 kDa