RCC1 (NM_001048195) Human Tagged ORF Clone
CAT#: RC219953
- TrueORF®
RCC1 (Myc-DDK-tagged)-Human regulator of chromosome condensation 1 (RCC1), transcript variant 2
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_001048195" in other vectors (4)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | RCC1 |
Synonyms | CHC1; RCC1-I; SNHG3-RCC1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC219953 representing NM_001048195
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCACCCAAGCGCATAGCTAAAAGAAGGTCCCCCCCAGCAGATGCCATCCCCAAAAGCAAGAAGGTGA AGGACACGAGGGCCGCTGCCTCCCGCCGCGTTCCTGGCGCCCGCTCCTGCCAAGTCTCACACAGGTCCCA CAGCACAGAACCCGGCTTGGTGCTGACACTAGGCCAGGGCGACGTGGGCCAGCTGGGGCTGGGTGAGAAT GTGATGGAGAGGAAGAAGCCGGCCCTGGTATCCATTCCGGAGGATGTTGTGCAGGCTGAGGCTGGGGGCA TGCACACCGTGTGTCTAAGCAAAAGTGGCCAGGTCTATTCCTTCGGCTGCAATGATGAGGGTGCCCTGGG AAGGGACACATCAGTGGAGGGCTCGGAGATGGTCCCTGGGAAAGTGGAGCTGCAAGAGAAGGTGGTACAG GTGTCAGCAGGAGACAGTCACACAGCAGCCCTCACCGATGATGGCCGTGTCTTCCTCTGGGGCTCCTTCC GGGACAATAACGGTGTGATTGGACTGTTGGAGCCCATGAAGAAGAGCATGGTGCCTGTGCAGGTGCAGCT GGATGTGCCTGTGGTAAAGGTGGCCTCAGGAAACGACCACTTGGTGATGCTGACAGCTGATGGTGACCTC TACACCTTGGGCTGCGGGGAACAGGGCCAGCTAGGCCGTGTGCCTGAGTTATTTGCCAACCGTGGTGGCC GGCAAGGCCTCGAACGACTCCTGGTCCCCAAGTGTGTGATGCTGAAATCCAGGGGAAGCCGGGGCCACGT GAGATTCCAGGATGCCTTTTGTGGTGCCTATTTCACCTTTGCCATCTCCCATGAGGGCCACGTGTACGGC TTCGGCCTCTCCAACTACCATCAGCTTGGAACTCCGGGCACAGAATCTTGCTTCATACCCCAGAACCTAA CATCCTTCAAGAATTCCACCAAGTCCTGGGTGGGCTTCTCTGGTGGCCAGCACCATACAGTCTGCATGGA TTCGGAAGGAAAAGCATACAGCCTGGGCCGGGCTGAGTATGGGCGGCTGGGCCTTGGAGAGGGTGCTGAG GAGAAGAGCATACCCACCCTCATCTCCAGGCTGCCTGCTGTCTCCTCGGTGGCTTGTGGGGCCTCTGTGG GGTATGCTGTGACCAAGGATGGTCGTGTTTTCGCCTGGGGCATGGGCACCAACTACCAGCTGGGCACAGG GCAGGATGAGGACGCCTGGAGCCCTGTGGAGATGATGGGCAAACAGCTGGAGAACCGTGTGGTCTTATCT GTGTCCAGCGGGGGCCAGCATACAGTCTTATTAGTCAAGGACAAAGAACAGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219953 representing NM_001048195
Red=Cloning site Green=Tags(s) MSPKRIAKRRSPPADAIPKSKKVKDTRAAASRRVPGARSCQVSHRSHSTEPGLVLTLGQGDVGQLGLGEN VMERKKPALVSIPEDVVQAEAGGMHTVCLSKSGQVYSFGCNDEGALGRDTSVEGSEMVPGKVELQEKVVQ VSAGDSHTAALTDDGRVFLWGSFRDNNGVIGLLEPMKKSMVPVQVQLDVPVVKVASGNDHLVMLTADGDL YTLGCGEQGQLGRVPELFANRGGRQGLERLLVPKCVMLKSRGSRGHVRFQDAFCGAYFTFAISHEGHVYG FGLSNYHQLGTPGTESCFIPQNLTSFKNSTKSWVGFSGGQHHTVCMDSEGKAYSLGRAEYGRLGLGEGAE EKSIPTLISRLPAVSSVACGASVGYAVTKDGRVFAWGMGTNYQLGTGQDEDAWSPVEMMGKQLENRVVLS VSSGGQHTVLLVKDKEQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001048195 |
ORF Size | 1314 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001048195.4 |
RefSeq Size | 2490 bp |
RefSeq ORF | 1317 bp |
Locus ID | 1104 |
UniProt ID | P18754 |
Cytogenetics | 1p35.3 |
MW | 46.6 kDa |
Gene Summary | Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis (PubMed:1944575, PubMed:17435751, PubMed:20668449, PubMed:22215983, PubMed:11336674). Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis (PubMed:12194828, PubMed:17435751, PubMed:22215983). Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import (PubMed:22215983). Involved in the regulation of onset of chromosome condensation in the S phase (PubMed:3678831). Binds both to the nucleosomes and double-stranded DNA (PubMed:17435751, PubMed:18762580).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC219953L3 | Lenti-ORF clone of RCC1 (Myc-DDK-tagged)-Human regulator of chromosome condensation 1 (RCC1), transcript variant 2 |
USD 757.00 |
|
RC219953L4 | Lenti-ORF clone of RCC1 (mGFP-tagged)-Human regulator of chromosome condensation 1 (RCC1), transcript variant 2 |
USD 757.00 |
|
RG219953 | RCC1 (tGFP-tagged) - Human regulator of chromosome condensation 1 (RCC1), transcript variant 2 |
USD 657.00 |
|
SC315432 | RCC1 (untagged)-Human regulator of chromosome condensation 1 (RCC1), transcript variant 2 |
USD 503.00 |
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