RCC1 (NM_001048195) Human Untagged Clone

CAT#: SC315432

RCC1 (untagged)-Human regulator of chromosome condensation 1 (RCC1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RCC1
• Synonyms: CHC1; RCC1-I; SNHG3-RCC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC315432 representing NM_001048195.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCACCCAAGCGCATAGCTAAAAGAAGGTCCCCCCCAGCAGATGCCATCCCCAAAAGCAAGAAGGTG
  AAGGACACGAGGGCCGCTGCCTCCCGCCGCGTTCCTGGCGCCCGCTCCTGCCAAGTCTCACACAGGTCC
  CACAGCACAGAACCCGGCTTGGTGCTGACACTAGGCCAGGGCGACGTGGGCCAGCTGGGGCTGGGTGAG
  AATGTGATGGAGAGGAAGAAGCCGGCCCTGGTATCCATTCCGGAGGATGTTGTGCAGGCTGAGGCTGGG
  GGCATGCACACCGTGTGTCTAAGCAAAAGTGGCCAGGTCTATTCCTTCGGCTGCAATGATGAGGGTGCC
  CTGGGAAGGGACACATCAGTGGAGGGCTCGGAGATGGTCCCTGGGAAAGTGGAGCTGCAAGAGAAGGTG
  GTACAGGTGTCAGCAGGAGACAGTCACACAGCAGCCCTCACCGATGATGGCCGTGTCTTCCTCTGGGGC
  TCCTTCCGGGACAATAACGGTGTGATTGGACTGTTGGAGCCCATGAAGAAGAGCATGGTGCCTGTGCAG
  GTGCAGCTGGATGTGCCTGTGGTAAAGGTGGCCTCAGGAAACGACCACTTGGTGATGCTGACAGCTGAT
  GGTGACCTCTACACCTTGGGCTGCGGGGAACAGGGCCAGCTAGGCCGTGTGCCTGAGTTATTTGCCAAC
  CGTGGTGGCCGGCAAGGCCTCGAACGACTCCTGGTCCCCAAGTGTGTGATGCTGAAATCCAGGGGAAGC
  CGGGGCCACGTGAGATTCCAGGATGCCTTTTGTGGTGCCTATTTCACCTTTGCCATCTCCCATGAGGGC
  CACGTGTACGGCTTCGGCCTCTCCAACTACCATCAGCTTGGAACTCCGGGCACAGAATCTTGCTTCATA
  CCCCAGAACCTAACATCCTTCAAGAATTCCACCAAGTCCTGGGTGGGCTTCTCTGGTGGCCAGCACCAT
  ACAGTCTGCATGGATTCGGAAGGAAAAGCATACAGCCTGGGCCGGGCTGAGTATGGGCGGCTGGGCCTT
  GGAGAGGGTGCTGAGGAGAAGAGCATACCCACCCTCATCTCCAGGCTGCCTGCTGTCTCCTCGGTGGCT
  TGTGGGGCCTCTGTGGGGTATGCTGTGACCAAGGATGGTCGTGTTTTCGCCTGGGGCATGGGCACCAAC
  TACCAGCTGGGCACAGGGCAGGATGAGGACGCCTGGAGCCCTGTGGAGATGATGGGCAAACAGCTGGAG
  AACCGTGTGGTCTTATCTGTGTCCAGCGGGGGCCAGCATACAGTCTTATTAGTCAAGGACAAAGAACAG
  AGCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001048195
• Insert Size: 1317 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001048195.2
• RefSeq Size: 2591 bp
• RefSeq ORF: 1317 bp
• Locus ID: 1104
• UniProt ID: P18754
• Cytogenetics: 1p35.3
• MW: 46.8 kDa
• Gene Summary: Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis (PubMed:1944575, PubMed:17435751, PubMed:20668449, PubMed:22215983, PubMed:11336674). Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis (PubMed:12194828, PubMed:17435751, PubMed:22215983). Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import (PubMed:22215983). Involved in the regulation of onset of chromosome condensation in the S phase (PubMed:3678831). Binds both to the nucleosomes and double-stranded DNA (PubMed:17435751, PubMed:18762580).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a.