TMEM180 (MFSD13A) (NM_024789) Human Tagged ORF Clone

CAT#: RC219636

TMEM180 (Myc-DDK-tagged)-Human transmembrane protein 180 (TMEM180)

Product Images

Circular map for RC219636

• 

  Western blot validation of overexpression lysate (Cat# LY411050) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219636 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMEM180
• Synonyms: bA18I14.8; C10orf77; TMEM180
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219636 representing NM_024789
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTGGGTCAGCCCCAGGCCTGGTTGCTGGGTCTGCCCACAGCTGTGGTCTATGGCTCCCTGGCTC
  TCTTCACCACCATCCTGCACAATGTCTTCCTGCTCTACTATGTGGACACCTTTGTCTCAGTGTACAAGAT
  CAACAAAATGGCCTTCTGGGTCGGAGAGACAGTGTTTCTCCTCTGGAACAGCCTCAATGACCCTCTCTTC
  GGTTGGCTCAGTGACCGGCAGTTCCTCAGCTCCCAGCCCCGGTCAGGCGCCGGGCTCTCCTCAAGGGCTG
  TGGTGCTGGCCCGGGTGCAGGCCCTGGGCTGGCATGGGCCGCTGCTGGCGCTGTCGTTCCTGGCGTTCTG
  GGTGCCCTGGGCCCCAGCTGGCCTGCAGTTCTTGCTGTGCCTGTGCCTCTATGATGGCTTCCTGACGCTC
  GTGGACCTGCACCACCATGCCTTGCTGGCCGACCTGGCCCTCTCAGCCCACGACCGCACCCACCTCAACT
  TCTACTGCTCCCTCTTCAGCGCGGCCGGCTCCCTCTCTGTCTTTGCATCCTATGCCTTTTGGAACAAGGA
  GGATTTCTCCTCCTTCCGCGCTTTCTGCGTGACACTGGCTGTCAGCTCTGGGCTGGGCTTTCTGGGGGCC
  ACACAGCTGCTGAGGCGGCGGGTTGAGGCGGCCCGAAAGGACCCAGGGTGCTCAGGCCTGGTTGTGGATA
  GCGGCCTGTGTGGAGAGGAGCTGCTTGTGGGCAGTGAGGAGGCGGACAGCATCACCTTGGGCCGGTATCT
  CCGGCAGCTGGCACGCCATCGGAACTTCCTGTGGTTCGTGAGCATGGACCTGGTGCAGGTCTTCCACTGC
  CACTTCAACAGCAACTTCTTCCCTCTCTTCCTGGAGCATCTGTTGTCCGACCATATCTCCCTTTCCACGG
  GCTCCATCCTGTTGGGCCTCTCCTATGTCGCTCCCCATCTCAACAACCTCTACTTCCTGTCCCTGTGCCG
  GCGCTGGGGCGTCTACGCGGTGGTGCGGGGGCTCTTCCTGCTCAAGCTGGGACTTAGCCTGCTCATGTTG
  TTGGCCGGCCCGGACCACCTCAGCCTGCTGTGCCTCTTCATTGCCAGCAACCGCGTCTTCACTGAGGGCA
  CCTGTAAGCTGCTGACCTTGGTGGTCACTGACCTGGTAGACGAGGACCTGGTGCTGAACCACCGCAAGCA
  GGCAGCCTCGGCACTCCTCTTTGGCATGGTTGCCTTGGTGACCAAGCCAGGCCAGACCTTTGCCCCGCTG
  CTGGGCACCTGGCTGCTCTGTTTCTACACAGGTCATGACCTCTTCCAGCAGTCCCTCATAACCCCTGTGG
  GGAGTGCCCATCCCTGGCCAGAGCCCCCAGCTCCAGCCCCTGCACAGGCCCCGACGCTCCGCCAGGGCTG
  CTTCTACCTGCTGGTGCTGGTGCCCATCACCTGTGCTCTGCTGCAGCTCTTCACCTGGTCCCAGTTCACG
  CTGCATGGGAGACGCCTGCACATGGTCAAGGCCCAGCGCCAGAACCTGTCACAGGCCCAAACCCTGGATG
  TTAAGATGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219636 representing NM_024789
  Red=Cloning site Green=Tags(s)
  
  MGLGQPQAWLLGLPTAVVYGSLALFTTILHNVFLLYYVDTFVSVYKINKMAFWVGETVFLLWNSLNDPLF
  GWLSDRQFLSSQPRSGAGLSSRAVVLARVQALGWHGPLLALSFLAFWVPWAPAGLQFLLCLCLYDGFLTL
  VDLHHHALLADLALSAHDRTHLNFYCSLFSAAGSLSVFASYAFWNKEDFSSFRAFCVTLAVSSGLGFLGA
  TQLLRRRVEAARKDPGCSGLVVDSGLCGEELLVGSEEADSITLGRYLRQLARHRNFLWFVSMDLVQVFHC
  HFNSNFFPLFLEHLLSDHISLSTGSILLGLSYVAPHLNNLYFLSLCRRWGVYAVVRGLFLLKLGLSLLML
  LAGPDHLSLLCLFIASNRVFTEGTCKLLTLVVTDLVDEDLVLNHRKQAASALLFGMVALVTKPGQTFAPL
  LGTWLLCFYTGHDLFQQSLITPVGSAHPWPEPPAPAPAQAPTLRQGCFYLLVLVPITCALLQLFTWSQFT
  LHGRRLHMVKAQRQNLSQAQTLDVKMV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024789
• ORF Size: 1551 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024789.2, NP_079065.2
• RefSeq Size: 2846 bp
• RefSeq ORF: 1554 bp
• Locus ID: 79847
• UniProt ID: Q14CX5
• Cytogenetics: 10q24.32
• Protein Families: Transmembrane
• MW: 57.2 kDa