MYO19 (NM_001033580) Human Tagged ORF Clone

CAT#: RC219483

MYO19 (Myc-DDK-tagged)-Human myosin XIX (MYO19), transcript variant 3

Product Images

Circular map for RC219483

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  Western blot validation of overexpression lysate (Cat# LY422404) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219483 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MYO19
• Synonyms: MYOHD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219483 representing NM_001033580
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCAGCAGGTCAATGGCCACAATCCGGGGTCTGATGGCCAAGCCAGGGAGTACCTCAGAGAAGACC
  TGCAGGAGTTCCTGGGTGGGGAGGTCCTGCTGTACAAACTGGATGACCTCACCAGGGTGAATCCTGTGAC
  ACTAGAGACAGTCCTGAGGTGCCTGCAGGCCCGGTACATGGCAGACACATTCTACACCAATGCTGGCTGC
  ACCCTGGTAGCCTTGAACCCCTTCAAGCCTGTTCCTCAGCTCTACTCGCCCGAGCTAATGAGAGAGTACC
  ATGCTGCGCCTCAGCCCCAGAAACTGAAGCCCCATGTGTTCACTGTGGGTGAACAGACCTACAGGAATGT
  CAAGAGCCTGATTGAACCAGTCAACCAGTCTATTGTTGTCAGTGGAGAGAGTGGTGCTGGAAAGACATGG
  ACGTCTCGCTGCCTAATGAAGTTCTATGCTGTGGTGGCCACCTCACCTGCATCTTGGGAGAGCCACAAGA
  TTGCAGAGAGGATAGAACAGAGGATCCTGAACTCCAACCCTGTCATGGAAGCTTTTGGGAATGCGTGTAC
  ACTGAGGAATAACAACAGCAGTCGCTTTGGGAAGTTCATCCAGCTCCAGCTGAACAGGGCTCAGCAAATG
  ACTGGAGCCGCAGTCCAGACCTACCTCCTAGAGAAAACTCGAGTGGCCTGCCAGGCTTCCAGTGAGAGGA
  ACTTCCACATCTTCTATCAGATTTGCAAAGGAGCCAGTGAGGACGAGAGGCTCCAGTGGCACCTTCCTGA
  GGGAGCTGCCTTCTCCTGGCTGCCCAACCCAGAGAGGAGCTTAGAAGAGGATTGTTTTGAGGTGACCAGA
  GAGGCCATGCTCCATTTGGGCATTGACACCCCTACCCAGAACAACATCTTTAAGGTCAGAAGAAAAGCCA
  CGCCACTTAAGTTTGGCAGAGATGACGGGCAGCCCTTTGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219483 representing NM_001033580
  Red=Cloning site Green=Tags(s)
  
  MLQQVNGHNPGSDGQAREYLREDLQEFLGGEVLLYKLDDLTRVNPVTLETVLRCLQARYMADTFYTNAGC
  TLVALNPFKPVPQLYSPELMREYHAAPQPQKLKPHVFTVGEQTYRNVKSLIEPVNQSIVVSGESGAGKTW
  TSRCLMKFYAVVATSPASWESHKIAERIEQRILNSNPVMEAFGNACTLRNNNSSRFGKFIQLQLNRAQQM
  TGAAVQTYLLEKTRVACQASSERNFHIFYQICKGASEDERLQWHLPEGAAFSWLPNPERSLEEDCFEVTR
  EAMLHLGIDTPTQNNIFKVRRKATPLKFGRDDGQPFA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033580
• ORF Size: 951 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033580.2, NP_001028752.1
• RefSeq Size: 1730 bp
• RefSeq ORF: 954 bp
• Locus ID: 80179
• UniProt ID: Q96H55
• Cytogenetics: 17q12
• MW: 35.8 kDa
• Gene Summary: Actin-based motor molecule with ATPase activity that localizes to the mitochondrion outer membrane (PubMed:19932026, PubMed:23568824, PubMed:25447992). Motor protein that moves towards the plus-end of actin filaments (By similarity). Required for mitochondrial inheritance during mitosis (PubMed:25447992). May be involved in mitochondrial transport or positioning (PubMed:23568824).[UniProtKB/Swiss-Prot Function]