PAIP2B (NM_020459) Human Tagged ORF Clone
CAT#: RC219314
PAIP2B (Myc-DDK-tagged)-Human poly(A) binding protein interacting protein 2B (PAIP2B)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_020459" in other vectors (4)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PAIP2B |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC219314 representing NM_020459
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGATCCAATATGGCAAATACATCACCGAGTGTAAAATCCAAAGAGGACCAGGGGTTAAGTGGGC ACGATGAAAAGGAAAACCCATTTGCAGAGTACATGTGGATGGAGAATGAAGAGGATTTCAACAGACAGGT GGAGGAGGAACTGCAGGAGCAAGACTTCTTGGACCGCTGCTTCCAAGAGATGCTGGATGAAGAAGACCAA GACTGGTTTATTCCCTCACGAGACCTGCCTCAGGCCATGGGACAGTTGCAACAGCAGTTAAATGGACTGT CAGTCAGTGAAGGTCATGATTCTGAAGATATTTTGAGCAAAAGTAACCTGAACCCAGATGCCAAGGAGTT TATTCCAGGAGAGAAGTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219314 representing NM_020459
Red=Cloning site Green=Tags(s) MNGSNMANTSPSVKSKEDQGLSGHDEKENPFAEYMWMENEEDFNRQVEEELQEQDFLDRCFQEMLDEEDQ DWFIPSRDLPQAMGQLQQQLNGLSVSEGHDSEDILSKSNLNPDAKEFIPGEKY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_020459 |
ORF Size | 369 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020459.1, NP_065192.1 |
RefSeq Size | 6300 bp |
RefSeq ORF | 372 bp |
Locus ID | 400961 |
UniProt ID | Q9ULR5 |
Cytogenetics | 2p13.3 |
MW | 14.1 kDa |
Gene Summary | Most mRNAs, except for histones, contain a 3-prime poly(A) tail. Poly(A)-binding protein (PABP; see MIM 604679) enhances translation by circularizing mRNA through its interaction with the translation initiation factor EIF4G1 (MIM 600495) and the poly(A) tail. Various PABP-binding proteins regulate PABP activity, including PAIP1 (MIM 605184), a translational stimulator, and PAIP2A (MIM 605604) and PAIP2B, translational inhibitors (Derry et al., 2006 [PubMed 17381337]).[supplied by OMIM, Mar 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC219314L3 | Lenti ORF clone of Human poly(A) binding protein interacting protein 2B (PAIP2B), Myc-DDK-tagged |
USD 450.00 |
|
RC219314L4 | Lenti ORF clone of Human poly(A) binding protein interacting protein 2B (PAIP2B), mGFP tagged |
USD 450.00 |
|
RG219314 | PAIP2B (tGFP-tagged) - Human poly(A) binding protein interacting protein 2B (PAIP2B) |
USD 350.00 |
|
SC316122 | PAIP2B (untagged)-Human poly(A) binding protein interacting protein 2B (PAIP2B) |
USD 165.00 |
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