TRPA1 (NM_007332) Human Tagged ORF Clone

CAT#: RC219290

TRPA1 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily A, member 1 (TRPA1)

Product Images

Circular map for RC219290

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRPA1
• Synonyms: ANKTM1; FEPS; FEPS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219290 representing NM_007332.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAGCGCAGCCTGAGGAAGATGTGGCGCCCTGGAGAAAAGAAGGAGCCCCAGGGCGTTGTCTATGAG
  GATGTGCCGGACGACACGGAGGATTTCAAGGAATCGCTTAAGGTGGTTTTTGAAGGAAGTGCATATGGA
  TTACAAAACTTTAATAAGCAAAAGAAATTAAAAAGATGTGACGATATGGACACCTTCTTCTTGCATTAT
  GCTGCAGCAGAAGGCCAAATTGAGCTAATGGAGAAGATCACCAGAGATTCCTCTTTGGAAGTGCTGCAT
  GAAATGGATGATTATGGAAATACCCCTCTGCATTGTGCTGTAGAAAAAAACCAAATTGAAAGCGTTAAG
  TTTCTTCTCAGCAGAGGAGCAAACCCAAACCTCCGAAACTTCAACATGATGGCTCCTCTCCACATAGCT
  GTGCAGGGCATGAATAATGAGGTGATGAAGGTCTTGCTTGAGCATAGAACTATTGATGTTAATTTGGAA
  GGAGAAAATGGAAACACAGCTGTGATCATTGCGTGCACCACAAATAATAGCGAAGCATTGCAGATTTTG
  CTTAACAAAGGAGCTAAGCCATGTAAATCAAATAAATGGGGATGTTTCCCTATTCACCAAGCTGCATTT
  TCAGGTTCCAAAGAATGCATGGAAATAATACTAAGGTTTGGTGAAGAGCATGGGTACAGTAGACAGTTG
  CACATTAACTTTATGAATAATGGGAAAGCCACCCCTCTCCACCTGGCTGTGCAAAATGGTGACTTGGAA
  ATGATCAAAATGTGCCTGGACAATGGTGCACAAATAGACCCAGTGGAGAAGGGAAGGTGCACAGCCATT
  CATTTTGCTGCCACCCAGGGAGCCACTGAGATTGTTAAACTGATGATATCGTCCTATTCTGGTAGCGTG
  GATATTGTTAACACAACCGATGGATGTCATGAGACCATGCTTCACAGAGCTTCATTGTTTGATCACCAT
  GAGCTAGCAGACTATTTAATTTCAGTGGGAGCAGATATTAATAAGATCGATTCTGAAGGACGCTCTCCA
  CTTATATTAGCAACTGCTTCTGCATCTTGGAATATTGTAAATTTGCTACTCTCTAAAGGTGCCCAAGTA
  GACATAAAAGATAATTTTGGACGTAATTTTCTGCATTTAACTGTACAGCAACCTTATGGATTAAAAAAT
  CTGCGACCTGAATTTATGCAGATGCAACAGATCAAAGAGCTGGTAATGGATGAAGACAACGATGGGTGT
  ACTCCTCTACATTATGCATGTAGACAGGGGGGCCCTGGTTCTGTAAATAACCTACTTGGCTTTAATGTG
  TCCATTCATTCCAAAAGCAAAGATAAGAAATCACCTCTGCATTTTGCAGCCAGTTATGGGCGTATCAAT
  ACCTGTCAGAGGCTCCTACAAGACATAAGTGATACGAGGCTTCTGAATGAAGGTGACCTTCATGGAATG
  ACTCCTCTCCATCTGGCAGCAAAGAATGGACATGATAAAGTAGTTCAGCTTCTTCTGAAAAAAGGTGCA
  TTGTTTCTCAGTGACCACAATGGCTGGACAGCTTTGCATCATGCGTCCATGGGCGGGTACACTCAGACC
  ATGAAGGTCATTCTTGATACTAATTTGAAGTGCACAGATCGCTTGGATGAAGACGGGAACACTGCACTT
  CACTTTGCTGCAAGGGAAGGCCATGCCAAAGCCGTTGCGCTTCTTCTGAGCCACAATGCTGACATAGTC
  CTGAACAAGCAGCAGGCCTCCTTTTTGCACCTTGCACTTCACAATAAGAGGAAGGAGGTTGTTCTTACG
  ATCATCAGGAGCAAAAGATGGGATGAATGTCTTAAGATTTTCAGTCATAATTCTCCAGGCAATAAATGT
  CCAATTACAGAAATGATAGAATACCTCCCTGAATGCATGAAGGTACTTTTAGATTTCTGCATGTTGCAT
  TCCACAGAAGACAAGTCCTGCCGAGACTATTATATCGAGTATAATTTCAAATATCTTCAATGTCCATTA
  GAATTCACCAAAAAAACACCTACACAGGATGTTATATATGAACCGCTTACAGCCCTCAACGCAATGGTA
  CAAAATAACCGCATAGAGCTTCTCAATCATCCTGTGTGTAAAGAATATTTACTCATGAAATGGTTGGCT
  TATGGATTTAGAGCTCATATGATGAATTTAGGATCTTACTGTCTTGGTCTCATACCTATGACCATTCTC
  GTTGTCAATATAAAACCAGGAATGGCTTTCAACTCAACTGGCATCATCAATGAAACTAGTGATCATTCA
  GAAATACTAGATACCACGAATTCATATCTAATAAAAACTTGTATGATTTTAGTGTTTTTATCAAGTATA
  TTTGGGTATTGCAAAGAAGCGGGGCAAATTTTCCAACAGAAAAGGAATTATTTTATGGATATAAGCAAT
  GTTCTTGAATGGATTATCTACACGACGGGCATCATTTTTGTGCTGCCCTTGTTTGTTGAAATACCAGCT
  CATCTGCAGTGGCAATGTGGAGCAATTGCTGTTTACTTCTATTGGATGAATTTCTTATTGTATCTTCAA
  AGATTTGAAAATTGTGGAATTTTTATTGTTATGTTGGAGGTAATTTTGAAAACTTTGTTGAGGTCTACA
  GTTGTATTTATCTTCCTTCTTCTGGCTTTTGGACTCAGCTTTTACATCCTCCTGAATTTACAGGATCCC
  TTCAGCTCTCCATTGCTTTCTATAATCCAGACCTTCAGCATGATGCTAGGAGATATCAATTATCGAGAG
  TCCTTCCTAGAACCATATCTGAGAAATGAATTGGCACATCCAGTTCTGTCCTTTGCACAACTTGTTTCC
  TTCACAATATTTGTCCCAATTGTCCTCATGAATTTACTTATTGGTTTGGCAGTTGGCGACATTGCTGAG
  GTCCAGAAACATGCATCATTGAAGAGGATAGCTATGCAGGTGGAACTTCATACCAGCTTAGAGAAGAAG
  CTGCCACTTTGGTTTCTACGCAAAGTGGATCAGAAATCCACCATCGTGTATCCCAACAAACCCAGATCT
  GGTGGGATGTTATTCCATATATTCTGTTTTTTATTTTGCACTGGGGAAATAAGACAAGAAATACCAAAT
  GCTGATAAATCTTTAGAAATGGAAATATTAAAGCAGAAATACCGGCTGAAGGATCTTACTTTTCTCCTG
  GAAAAACAGCATGAGCTCATTAAACTGATCATTCAGAAGATGGAGATCATCTCTGAGACAGAGGATGAT
  GATAGCCATTGTTCTTTTCAAGACAGGTTTAAGAAAGAGCAGATGGAACAAAGGAATAGCAGATGGAAT
  ACTGTGTTGAGAGCAGTCAAGGCAAAAACACACCATCTTGAGCCT
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by RC219290
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MKRSLRKMWRPGEKKEPQGVVYEDVPDDTEDFKESLKVVFEGSAYGLQNFNKQKKLKRCDDMDTFFLHY
  AAAEGQIELMEKITRDSSLEVLHEMDDYGNTPLHCAVEKNQIESVKFLLSRGANPNLRNFNMMAPLHIA
  VQGMNNEVMKVLLEHRTIDVNLEGENGNTAVIIACTTNNSEALQILLNKGAKPCKSNKWGCFPIHQAAF
  SGSKECMEIILRFGEEHGYSRQLHINFMNNGKATPLHLAVQNGDLEMIKMCLDNGAQIDPVEKGRCTAI
  HFAATQGATEIVKLMISSYSGSVDIVNTTDGCHETMLHRASLFDHHELADYLISVGADINKIDSEGRSP
  LILATASASWNIVNLLLSKGAQVDIKDNFGRNFLHLTVQQPYGLKNLRPEFMQMQQIKELVMDEDNDGC
  TPLHYACRQGGPGSVNNLLGFNVSIHSKSKDKKSPLHFAASYGRINTCQRLLQDISDTRLLNEGDLHGM
  TPLHLAAKNGHDKVVQLLLKKGALFLSDHNGWTALHHASMGGYTQTMKVILDTNLKCTDRLDEDGNTAL
  HFAAREGHAKAVALLLSHNADIVLNKQQASFLHLALHNKRKEVVLTIIRSKRWDECLKIFSHNSPGNKC
  PITEMIEYLPECMKVLLDFCMLHSTEDKSCRDYYIEYNFKYLQCPLEFTKKTPTQDVIYEPLTALNAMV
  QNNRIELLNHPVCKEYLLMKWLAYGFRAHMMNLGSYCLGLIPMTILVVNIKPGMAFNSTGIINETSDHS
  EILDTTNSYLIKTCMILVFLSSIFGYCKEAGQIFQQKRNYFMDISNVLEWIIYTTGIIFVLPLFVEIPA
  HLQWQCGAIAVYFYWMNFLLYLQRFENCGIFIVMLEVILKTLLRSTVVFIFLLLAFGLSFYILLNLQDP
  FSSPLLSIIQTFSMMLGDINYRESFLEPYLRNELAHPVLSFAQLVSFTIFVPIVLMNLLIGLAVGDIAE
  VQKHASLKRIAMQVELHTSLEKKLPLWFLRKVDQKSTIVYPNKPRSGGMLFHIFCFLFCTGEIRQEIPN
  ADKSLEMEILKQKYRLKDLTFLLEKQHELIKLIIQKMEIISETEDDDSHCSFQDRFKKEQMEQRNSRWN
  TVLRAVKAKTHHLEP
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007332
• ORF Size: 3357 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 5190 bp
• RefSeq ORF: 3360 bp
• Locus ID: 8989
• UniProt ID: O75762
• Cytogenetics: 8q21.11
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• MW: 127.5 kDa
• Gene Summary: The structure of the protein encoded by this gene is highly related to both the protein ankyrin and transmembrane proteins. The specific function of this protein has not yet been determined; however, studies indicate the function may involve a role in signal transduction and growth control. [provided by RefSeq, Jul 2008]