TRPA1 (NM_007332) Human Tagged ORF Clone

CAT#: RG219290

TRPA1 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily A, member 1 (TRPA1)

Product Images

Circular map for RG219290

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TRPA1
• Synonyms: ANKTM1; FEPS; FEPS1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219290 representing NM_007332
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCGCAGCCTGAGGAAGATGTGGCGCCCTGGAGAAAAGAAGGAGCCCCAGGGCGTTGTCTATGAGG
  ATGTGCCGGACGACACGGAGGATTTCAAGGAATCGCTTAAGGTGGTTTTTGAAGGAAGTGCATATGGATT
  ACAAAACTTTAATAAGCAAAAGAAATTAAAAAGATGTGACGATATGGACACCTTCTTCTTGCATTATGCT
  GCAGCAGAAGGCCAAATTGAGCTAATGGAGAAGATCACCAGAGATTCCTCTTTGGAAGTGCTGCATGAAA
  TGGATGATTATGGAAATACCCCTCTGCATTGTGCTGTAGAAAAAAACCAAATTGAAAGCGTTAAGTTTCT
  TCTCAGCAGAGGAGCAAACCCAAACCTCCGAAACTTCAACATGATGGCTCCTCTCCACATAGCTGTGCAG
  GGCATGAATAATGAGGTGATGAAGGTCTTGCTTGAGCATAGAACTATTGATGTTAATTTGGAAGGAGAAA
  ATGGAAACACAGCTGTGATCATTGCGTGCACCACAAATAATAGCGAAGCATTGCAGATTTTGCTTAACAA
  AGGAGCTAAGCCATGTAAATCAAATAAATGGGGATGTTTCCCTATTCACCAAGCTGCATTTTCAGGTTCC
  AAAGAATGCATGGAAATAATACTAAGGTTTGGTGAAGAGCATGGGTACAGTAGACAGTTGCACATTAACT
  TTATGAATAATGGGAAAGCCACCCCTCTCCACCTGGCTGTGCAAAATGGTGACTTGGAAATGATCAAAAT
  GTGCCTGGACAATGGTGCACAAATAGACCCAGTGGAGAAGGGAAGGTGCACAGCCATTCATTTTGCTGCC
  ACCCAGGGAGCCACTGAGATTGTTAAACTGATGATATCGTCCTATTCTGGTAGCGTGGATATTGTTAACA
  CAACCGATGGATGTCATGAGACCATGCTTCACAGAGCTTCATTGTTTGATCACCATGAGCTAGCAGACTA
  TTTAATTTCAGTGGGAGCAGATATTAATAAGATCGATTCTGAAGGACGCTCTCCACTTATATTAGCAACT
  GCTTCTGCATCTTGGAATATTGTAAATTTGCTACTCTCTAAAGGTGCCCAAGTAGACATAAAAGATAATT
  TTGGACGTAATTTTCTGCATTTAACTGTACAGCAACCTTATGGATTAAAAAATCTGCGACCTGAATTTAT
  GCAGATGCAACAGATCAAAGAGCTGGTAATGGATGAAGACAACGATGGGTGTACTCCTCTACATTATGCA
  TGTAGACAGGGGGGCCCTGGTTCTGTAAATAACCTACTTGGCTTTAATGTGTCCATTCATTCCAAAAGCA
  AAGATAAGAAATCACCTCTGCATTTTGCAGCCAGTTATGGGCGTATCAATACCTGTCAGAGGCTCCTACA
  AGACATAAGTGATACGAGGCTTCTGAATGAAGGTGACCTTCATGGAATGACTCCTCTCCATCTGGCAGCA
  AAGAATGGACATGATAAAGTAGTTCAGCTTCTTCTGAAAAAAGGTGCATTGTTTCTCAGTGACCACAATG
  GCTGGACAGCTTTGCATCATGCGTCCATGGGCGGGTACACTCAGACCATGAAGGTCATTCTTGATACTAA
  TTTGAAGTGCACAGATCGCTTGGATGAAGACGGGAACACTGCACTTCACTTTGCTGCAAGGGAAGGCCAT
  GCCAAAGCCGTTGCGCTTCTTCTGAGCCACAATGCTGACATAGTCCTGAACAAGCAGCAGGCCTCCTTTT
  TGCACCTTGCACTTCACAATAAGAGGAAGGAGGTTGTTCTTACGATCATCAGGAGCAAAAGATGGGATGA
  ATGTCTTAAGATTTTCAGTCATAATTCTCCAGGCAATAAATGTCCAATTACAGAAATGATAGAATACCTC
  CCTGAATGCATGAAGGTACTTTTAGATTTCTGCATGTTGCATTCCACAGAAGACAAGTCCTGCCGAGACT
  ATTATATCGAGTATAATTTCAAATATCTTCAATGTCCATTAGAATTCACCAAAAAAACACCTACACAGGA
  TGTTATATATGAACCGCTTACAGCCCTCAACGCAATGGTACAAAATAACCGCATAGAGCTTCTCAATCAT
  CCTGTGTGTAAAGAATATTTACTCATGAAATGGTTGGCTTATGGATTTAGAGCTCATATGATGAATTTAG
  GATCTTACTGTCTTGGTCTCATACCTATGACCATTCTCGTTGTCAATATAAAACCAGGAATGGCTTTCAA
  CTCAACTGGCATCATCAATGAAACTAGTGATCATTCAGAAATACTAGATACCACGAATTCATATCTAATA
  AAAACTTGTATGATTTTAGTGTTTTTATCAAGTATATTTGGGTATTGCAAAGAAGCGGGGCAAATTTTCC
  AACAGAAAAGGAATTATTTTATGGATATAAGCAATGTTCTTGAATGGATTATCTACACGACGGGCATCAT
  TTTTGTGCTGCCCTTGTTTGTTGAAATACCAGCTCATCTGCAGTGGCAATGTGGAGCAATTGCTGTTTAC
  TTCTATTGGATGAATTTCTTATTGTATCTTCAAAGATTTGAAAATTGTGGAATTTTTATTGTTATGTTGG
  AGGTAATTTTGAAAACTTTGTTGAGGTCTACAGTTGTATTTATCTTCCTTCTTCTGGCTTTTGGACTCAG
  CTTTTACATCCTCCTGAATTTACAGGATCCCTTCAGCTCTCCATTGCTTTCTATAATCCAGACCTTCAGC
  ATGATGCTAGGAGATATCAATTATCGAGAGTCCTTCCTAGAACCATATCTGAGAAATGAATTGGCACATC
  CAGTTCTGTCCTTTGCACAACTTGTTTCCTTCACAATATTTGTCCCAATTGTCCTCATGAATTTACTTAT
  TGGTTTGGCAGTTGGCGACATTGCTGAGGTCCAGAAACATGCATCATTGAAGAGGATAGCTATGCAGGTG
  GAACTTCATACCAGCTTAGAGAAGAAGCTGCCACTTTGGTTTCTACGCAAAGTGGATCAGAAATCCACCA
  TCGTGTATCCCAACAAACCCAGATCTGGTGGGATGTTATTCCATATATTCTGTTTTTTATTTTGCACTGG
  GGAAATAAGACAAGAAATACCAAATGCTGATAAATCTTTAGAAATGGAAATATTAAAGCAGAAATACCGG
  CTGAAGGATCTTACTTTTCTCCTGGAAAAACAGCATGAGCTCATTAAACTGATCATTCAGAAGATGGAGA
  TCATCTCTGAGACAGAGGATGATGATAGCCATTGTTCTTTTCAAGACAGGTTTAAGAAAGAGCAGATGGA
  ACAAAGGAATAGCAGATGGAATACTGTGTTGAGAGCAGTCAAGGCAAAAACACACCATCTTGAGCCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219290 representing NM_007332
  Red=Cloning site Green=Tags(s)
  
  MKRSLRKMWRPGEKKEPQGVVYEDVPDDTEDFKESLKVVFEGSAYGLQNFNKQKKLKRCDDMDTFFLHYA
  AAEGQIELMEKITRDSSLEVLHEMDDYGNTPLHCAVEKNQIESVKFLLSRGANPNLRNFNMMAPLHIAVQ
  GMNNEVMKVLLEHRTIDVNLEGENGNTAVIIACTTNNSEALQILLNKGAKPCKSNKWGCFPIHQAAFSGS
  KECMEIILRFGEEHGYSRQLHINFMNNGKATPLHLAVQNGDLEMIKMCLDNGAQIDPVEKGRCTAIHFAA
  TQGATEIVKLMISSYSGSVDIVNTTDGCHETMLHRASLFDHHELADYLISVGADINKIDSEGRSPLILAT
  ASASWNIVNLLLSKGAQVDIKDNFGRNFLHLTVQQPYGLKNLRPEFMQMQQIKELVMDEDNDGCTPLHYA
  CRQGGPGSVNNLLGFNVSIHSKSKDKKSPLHFAASYGRINTCQRLLQDISDTRLLNEGDLHGMTPLHLAA
  KNGHDKVVQLLLKKGALFLSDHNGWTALHHASMGGYTQTMKVILDTNLKCTDRLDEDGNTALHFAAREGH
  AKAVALLLSHNADIVLNKQQASFLHLALHNKRKEVVLTIIRSKRWDECLKIFSHNSPGNKCPITEMIEYL
  PECMKVLLDFCMLHSTEDKSCRDYYIEYNFKYLQCPLEFTKKTPTQDVIYEPLTALNAMVQNNRIELLNH
  PVCKEYLLMKWLAYGFRAHMMNLGSYCLGLIPMTILVVNIKPGMAFNSTGIINETSDHSEILDTTNSYLI
  KTCMILVFLSSIFGYCKEAGQIFQQKRNYFMDISNVLEWIIYTTGIIFVLPLFVEIPAHLQWQCGAIAVY
  FYWMNFLLYLQRFENCGIFIVMLEVILKTLLRSTVVFIFLLLAFGLSFYILLNLQDPFSSPLLSIIQTFS
  MMLGDINYRESFLEPYLRNELAHPVLSFAQLVSFTIFVPIVLMNLLIGLAVGDIAEVQKHASLKRIAMQV
  ELHTSLEKKLPLWFLRKVDQKSTIVYPNKPRSGGMLFHIFCFLFCTGEIRQEIPNADKSLEMEILKQKYR
  LKDLTFLLEKQHELIKLIIQKMEIISETEDDDSHCSFQDRFKKEQMEQRNSRWNTVLRAVKAKTHHLEP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007332
• ORF Size: 3357 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007332.1, NP_015628.1
• RefSeq Size: 5190 bp
• RefSeq ORF: 3360 bp
• Locus ID: 8989
• UniProt ID: O75762
• Cytogenetics: 8q21.11
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• Gene Summary: The structure of the protein encoded by this gene is highly related to both the protein ankyrin and transmembrane proteins. The specific function of this protein has not yet been determined; however, studies indicate the function may involve a role in signal transduction and growth control. [provided by RefSeq, Jul 2008]