TRPC4 (NM_016179) Human Tagged ORF Clone

CAT#: RC218941

TRPC4 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha

Product Images

Circular map for RC218941

• 

  Western blot validation of overexpression lysate (Cat# LY402514) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218941 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRPC4
• Synonyms: HTRP-4; HTRP4; TRP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218941 representing NM_016179
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGTTCTATTACAAAAGAAATGTTAATGCTCCCTATAGAGACCGCATCCCTCTAAGGATAGTAA
  GAGCAGAATCAGAACTCTCGCCATCAGAAAAAGCCTACTTGAATGCTGTGGAAAAGGGAGATTATGCCAG
  TGTCAAGAAATCCCTAGAGGAAGCTGAAATTTATTTTAAAATCAATATTAATTGCATTGATCCTCTCGGA
  AGAACTGCTCTCCTCATTGCAATTGAAAATGAGAACTTGGAGCTCATCGAACTACTCTTAAGCTTTAATG
  TCTATGTTGGAGATGCTCTATTACATGCTATCAGAAAAGAAGTCGTCGGAGCTGTTGAGCTGTTATTGAA
  CCACAAAAAACCTAGTGGAGAAAAACAGGTGCCTCCTATACTCCTTGATAAGCAGTTCTCTGAATTCACT
  CCAGACATTACACCAATCATTTTGGCAGCCCATACAAATAATTATGAGATAATAAAACTCTTGGTTCAGA
  AAGGAGTCTCAGTGCCTCGACCCCACGAGGTCCGCTGTAACTGTGTGGAATGCGTGTCCAGTTCAGATGT
  GGACAGCCTCCGTCACTCACGCTCCAGACTCAACATCTACAAGGCCTTGGCCAGTCCCTCTCTCATTGCA
  CTGTCAAGCGAAGATCCTTTTCTCACAGCCTTTCAGTTAAGTTGGGAACTTCAGGAACTGAGCAAGGTGG
  AAAATGAATTCAAGTCGGAGTATGAAGAGCTGTCACGGCAGTGCAAACAATTTGCTAAGGACCTACTGGA
  TCAGACGAGAAGTTCCAGAGAACTGGAAATCATTCTTAATTACCGAGATGACAATAGTCTCATAGAAGAA
  CAAAGTGGAAATGATCTTGCAAGACTAAAATTGGCCATTAAGTACCGTCAAAAAGAGTTTGTTGCCCAGC
  CCAATTGTCAACAGCTGCTGGCATCTCGCTGGTACGATGAGTTTCCAGGCTGGAGGAGAAGACACTGGGC
  AGTGAAGATGGTGACATGTTTCATAATAGGACTTCTTTTTCCTGTCTTCTCTGTGTGCTACCTGATAGCT
  CCCAAAAGCCCACTTGGACTGTTCATCAGGAAGCCATTTATCAAGTTTATCTGCCACACAGCCTCCTATT
  TGACTTTTTTGTTCCTGCTGCTGCTTGCCTCTCAGCACATCGACAGGTCAGACTTGAACAGGCAAGGTCC
  ACCACCAACCATCGTCGAGTGGATGATATTACCGTGGGTCCTGGGCTTCATATGGGGAGAAATTAAACAG
  ATGTGGGATGGCGGACTTCAGGACTACATCCATGATTGGTGGAATCTAATGGACTTTGTAATGAACTCCT
  TATATTTAGCAACAATCTCCTTGAAAATTGTTGCATTTGTAAAGTACAGTGCCCTTAATCCACGAGAATC
  ATGGGACATGTGGCATCCCACTCTGGTGGCAGAGGCTTTATTTGCTATTGCAAACATCTTCAGTTCTCTG
  CGTCTGATCTCACTGTTTACTGCAAATTCTCACCTGGGACCTCTGCAAATATCTCTGGGAAGAATGCTCC
  TGGACATTTTGAAGTTTCTATTCATATACTGCCTTGTGTTGCTAGCATTTGCAAATGGCCTAAATCAATT
  GTACTTCTATTATGAAGAAACGAAAGGGTTAACCTGCAAAGGCATAAGATGTGAAAAGCAGAATAATGCA
  TTTTCAACGTTATTTGAGACACTGCAGTCCCTGTTTTGGTCAATATTTGGGCTCATCAATTTATATGTGA
  CCAATGTCAAAGCACAGCATGAATTTACTGAGTTTGTTGGTGCCACCATGTTTGGGACATACAATGTCAT
  CTCTCTGGTTGTTCTACTCAACATGTTAATAGCTATGATGAATAATTCTTACCAACTGATTGCTGACCAT
  GCAGATATAGAATGGAAATTTGCACGAACAAAGCTTTGGATGAGTTATTTTGAAGAAGGAGGTACTCTGC
  CTACTCCCTTCAATGTCATCCCGAGCCCCAAGTCTCTCTGGTACCTGATCAAATGGATCTGGACACACTT
  GTGCAAGAAAAAGATGAGAAGAAAGCCAGAAAGTTTTGGAACAATAGGGAGGCGAGCTGCTGATAACTTG
  AGAAGACATCACCAATACCAAGAAGTTATGAGGAACCTGGTGAAGCGATACGTTGCTGCAATGATTAGAG
  ATGCTAAAACTGAAGAAGGCCTGACCGAAGAGAACTTTAAGGAACTAAAGCAAGACATTTCTAGTTTCCG
  CTTTGAAGTCCTGGGATTACTAAGAGGAAGCAAACTTTCCACAATACAATCTGCGAATGCCTCGAAGGAG
  TCTTCAAATTCGGCAGACTCAGATGAAAAGAGTGATAGCGAAGGTAATAGCAAGGACAAGAAAAAGAATT
  TCAGCCTTTTTGATTTAACCACCCTGATTCATCCGAGATCAGCAGCAATTGCCTCTGAAAGACATAACAT
  AAGCAATGGCTCTGCCCTGGTGGTTCAGGAGCCGCCCAGGGAGAAGCAGAGAAAAGTGAATTTTGTGACC
  GATATCAAAAACTTTGGGTTATTTCATAGACGATCAAAACAAAATGCTGCTGAGCAAAATGCAAACCAAA
  TCTTCTCTGTTTCAGAAGAAGTTGCTCGTCAACAGGCTGCAGGACCACTTGAGAGAAATATTCAACTGGA
  ATCTCGAGGATTAGCTTCACGGGGTGACCTGAGCATTCCCGGTCTCAGTGAACAATGTGTGTTAGTAGAC
  CATAGAGAAAGGAATACGGACACACTGGGGTTACAGGTAGGAAAGAGAGTGTGTCCATTCAAGTCAGAGA
  AGGTGGTGGTGGAGGACACGGTTCCTATAATACCAAAGGAGAAACATGCAAAAGAAGAGGACTCTAGTAT
  AGACTATGATCTAAACCTCCCAGACACAGTCACCCACGAAGATTACGTGACCACAAGATTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218941 representing NM_016179
  Red=Cloning site Green=Tags(s)
  
  MAQFYYKRNVNAPYRDRIPLRIVRAESELSPSEKAYLNAVEKGDYASVKKSLEEAEIYFKININCIDPLG
  RTALLIAIENENLELIELLLSFNVYVGDALLHAIRKEVVGAVELLLNHKKPSGEKQVPPILLDKQFSEFT
  PDITPIILAAHTNNYEIIKLLVQKGVSVPRPHEVRCNCVECVSSSDVDSLRHSRSRLNIYKALASPSLIA
  LSSEDPFLTAFQLSWELQELSKVENEFKSEYEELSRQCKQFAKDLLDQTRSSRELEIILNYRDDNSLIEE
  QSGNDLARLKLAIKYRQKEFVAQPNCQQLLASRWYDEFPGWRRRHWAVKMVTCFIIGLLFPVFSVCYLIA
  PKSPLGLFIRKPFIKFICHTASYLTFLFLLLLASQHIDRSDLNRQGPPPTIVEWMILPWVLGFIWGEIKQ
  MWDGGLQDYIHDWWNLMDFVMNSLYLATISLKIVAFVKYSALNPRESWDMWHPTLVAEALFAIANIFSSL
  RLISLFTANSHLGPLQISLGRMLLDILKFLFIYCLVLLAFANGLNQLYFYYEETKGLTCKGIRCEKQNNA
  FSTLFETLQSLFWSIFGLINLYVTNVKAQHEFTEFVGATMFGTYNVISLVVLLNMLIAMMNNSYQLIADH
  ADIEWKFARTKLWMSYFEEGGTLPTPFNVIPSPKSLWYLIKWIWTHLCKKKMRRKPESFGTIGRRAADNL
  RRHHQYQEVMRNLVKRYVAAMIRDAKTEEGLTEENFKELKQDISSFRFEVLGLLRGSKLSTIQSANASKE
  SSNSADSDEKSDSEGNSKDKKKNFSLFDLTTLIHPRSAAIASERHNISNGSALVVQEPPREKQRKVNFVT
  DIKNFGLFHRRSKQNAAEQNANQIFSVSEEVARQQAAGPLERNIQLESRGLASRGDLSIPGLSEQCVLVD
  HRERNTDTLGLQVGKRVCPFKSEKVVVEDTVPIIPKEKHAKEEDSSIDYDLNLPDTVTHEDYVTTRL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016179
• ORF Size: 2931 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016179.4
• RefSeq Size: 3440 bp
• RefSeq ORF: 2934 bp
• Locus ID: 7223
• UniProt ID: Q9UBN4
• Cytogenetics: 13q13.3
• Domains: ANK, ion_trans
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• MW: 111.9 kDa
• Gene Summary: This gene encodes a member of the canonical subfamily of transient receptor potential cation channels. The encoded protein forms a non-selective calcium-permeable cation channel that is activated by Gq-coupled receptors and tyrosine kinases, and plays a role in multiple processes including endothelial permeability, vasodilation, neurotransmitter release and cell proliferation. Single nucleotide polymorphisms in this gene may be associated with generalized epilepsy with photosensitivity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]