ANKRD10 (NM_017664) Human Tagged ORF Clone

CAT#: RC218904

ANKRD10 (Myc-DDK-tagged)-Human ankyrin repeat domain 10 (ANKRD10)

Product Images

Circular map for RC218904

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  Western blot validation of overexpression lysate (Cat# LY413661) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218904 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ANKRD10 protein (Cat# TP318904). The protein was produced from HEK293T cells transfected with ANKRD10 cDNA clone (Cat# RC218904) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANKRD10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218904 representing NM_017664
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCGGCGGGAGCGGGCGCGGGCGTAGAGGCGGGCTTCTCCAGCGAGGAGCTGCTCTCGCTCCGTT
  TCCCGCTGCACCGCGCCTGCCGCGACGGGGACCTGGCCACGCTCTGCTCGCTGCTGCAGCAGACACCCCA
  CGCCCACCTGGCCTCTGAGGACTCCTTCTATGGCTGGACGCCCGTGCACTGGGCCGCGCATTTCGGCAAG
  TTGGAGTGCTTAGTGCAGTTGGTGAGAGCGGGAGCCACACTCAACGTCTCCACCACACGGTACGCGCAGA
  CGCCAGCCCACATTGCAGCCTTTGGGGGACATCCTCAGTGCCTGGTCTGGCTGATTCAAGCAGGAGCCAA
  CATTAACAAACCGGATTGTGAGGGTGAAACTCCCATTCACAAGGCAGCTCGCTCTGGGAGCCTAGAATGC
  ATCAGTGCCCTTGTGGCGAATGGGGCTCACGTCGACCTGAGAAATGCCAGTGGCCTGACAGCAGCAGACA
  TTGCACAAACCCAGGGTTTCCAAGAGTGTGCCCAGTTTCTCTTGAACCTCCAGAATTGTCATCTGAACCA
  TTTCTATAACAATGGCATCTTAAATGGGGGTCATCAGAATGTATTTCCTAATCATATTAGTGTGGGAACA
  AATCGAAAGAGATGCTTGGAAGACTCAGAAGACTTTGGAGTAAAGAAAGCTAGAACTGAAGCTCAAAGCT
  TGGATTCTGCCGTGCCACTCACGAATGGCGACACAGAAGACGATGCTGACAAAATGCACGTTGATAGGGA
  GTTTGCTGTTGTAACAGATATGAAAAACAGTAGCTCCGTATCGAATACATTGACAAATGGATGTGTCATC
  AATGGACATTTGGACTTCCCCTCCACGACCCCGCTCAGTGGGATGGAAAGCAGGAATGGCCAGTGCTTGA
  CAGGAACTAACGGAATTAGCAGTGGATTAGCCCCAGGACAGCCGTTTCCGAGTAGCCAGGGTTCTCTCTG
  CATTAGTGGGACTGAGGAGCCAGAGAAGACCCTGAGAGCTAACCCTGAGTTGTGCGGTTCTCTGCACCTG
  AACGGGAGTCCAAGTAGCTGCATAGCCAGTAGGCCTTCCTGGGTGGAAGACATTGGGGATAACCTGTACT
  ATGGACACTACCACGGGTTTGGGGACACTGCTGAAAGCATCCCAGAACTGAACAGTGTGGTCGAGCATTC
  CAAGTCCGTGAAGGTGCAGGAGCGGTACGACAGTGCCGTGCTGGGCACCATGCACCTGCACCACGGCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218904 representing NM_017664
  Red=Cloning site Green=Tags(s)
  
  MSAAGAGAGVEAGFSSEELLSLRFPLHRACRDGDLATLCSLLQQTPHAHLASEDSFYGWTPVHWAAHFGK
  LECLVQLVRAGATLNVSTTRYAQTPAHIAAFGGHPQCLVWLIQAGANINKPDCEGETPIHKAARSGSLEC
  ISALVANGAHVDLRNASGLTAADIAQTQGFQECAQFLLNLQNCHLNHFYNNGILNGGHQNVFPNHISVGT
  NRKRCLEDSEDFGVKKARTEAQSLDSAVPLTNGDTEDDADKMHVDREFAVVTDMKNSSSVSNTLTNGCVI
  NGHLDFPSTTPLSGMESRNGQCLTGTNGISSGLAPGQPFPSSQGSLCISGTEEPEKTLRANPELCGSLHL
  NGSPSSCIASRPSWVEDIGDNLYYGHYHGFGDTAESIPELNSVVEHSKSVKVQERYDSAVLGTMHLHHGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017664
• ORF Size: 1260 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017664.4
• RefSeq Size: 2495 bp
• RefSeq ORF: 1263 bp
• Locus ID: 55608
• UniProt ID: Q9NXR5
• Cytogenetics: 13q34
• Domains: ANK
• MW: 44.6 kDa