ZNF423 (NM_015069) Human Tagged ORF Clone

CAT#: RC218703

ZNF423 (Myc-DDK-tagged)-Human zinc finger protein 423 (ZNF423)

Product Images

Circular map for RC218703

• 

  Western blot validation of overexpression lysate (Cat# LY414807) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218703 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF423
• Synonyms: Ebfaz; hOAZ; JBTS19; NPHP14; OAZ; Roaz; Zfp104; ZFP423
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218703 representing NM_015069.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCATAAGAAGAGGGTTGAAGAGGGGGAGGCCTCAGACTTCTCGCTGGCCTGGGATTCCTCCGTGACA
  GCAGCAGGAGGCCTAGAAGGAGAGCCAGAGTGCGATCAGAAAACCAGCCGTGCGCTGGAAGACAGGAAC
  AGCGTGACAAGTCAAGAGGAGAGAAATGAGGATGATGAAGACATGGAGGATGAATCAATTTACACCTGC
  GATCACTGTCAGCAGGACTTCGAGTCTCTGGCAGACCTGACGGACCACCGGGCCCACCGCTGTCCTGGA
  GATGGTGATGACGACCCACAACTCTCCTGGGTGGCCTCGTCTCCCTCCAGCAAGGATGTTGCGTCACCC
  ACGCAGATGATCGGAGATGGTTGTGACCTCGGCCTCGGCGAGGAGGAAGGGGGCACGGGCCTGCCATAC
  CCTTGCCAGTTCTGCGACAAGTCCTTCATCCGCTTGAGCTACTTGAAGAGGCACGAGCAGATCCACAGC
  GACAAGCTGCCGTTCAAGTGCACCTACTGCAGCCGCCTCTTCAAGCACAAGAGGAGCCGCGACCGGCAC
  ATCAAGCTGCATACGGGCGACAAGAAGTATCACTGCCACGAGTGCGAGGCAGCCTTCTCCCGCAGCGAC
  CACCTCAAGATCCACCTGAAGACCCACAGCTCCAGCAAGCCCTTCAAGTGCACTGTGTGCAAGCGCGGC
  TTCTCCTCCACCAGCTCGCTGCAGAGCCACATGCAGGCCCACAAAAAGAACAAGGAGCATCTGGCCAAG
  TCGGAGAAGGAAGCCAAGAAGGACGACTTCATGTGCGACTACTGCGAGGACACCTTCAGCCAGACGGAG
  GAGCTGGAGAAGCACGTGCTCACCCGCCACCCGCAGCTGTCCGAGAAGGCGGACCTGCAGTGCATTCAC
  TGCCCTGAGGTCTTCGTCGACGAGAACACACTGCTCGCCCATATCCACCAAGCCCACGCCAACCAGAAA
  CACAAGTGCCCCATGTGCCCTGAGCAGTTCTCCTCAGTGGAAGGTGTCTACTGCCACCTGGACAGCCAC
  CGGCAGCCCGACTCCAGCAACCACAGTGTCAGTCCCGACCCTGTACTGGGCAGCGTGGCCTCCATGAGC
  AGCGCCACACCCGACTCCAGCGCCTCTGTGGAGCGTGGCTCCACCCCGGACTCCACCTTGAAGCCGCTG
  CGGGGGCAGAAGAAGATGCGGGATGACGGGCAGGGCTGGACCAAGGTGGTCTATAGCTGCCCCTATTGT
  TCCAAGCGGGACTTTAACAGCCTGGCCGTGCTGGAGATCCACCTGAAGACCATCCACGCGGACAAGCCC
  CAGCAGAGCCACACATGTCAGATCTGCCTGGACTCCATGCCCACCCTCTACAACCTCAACGAGCACGTT
  CGCAAGCTGCACAAGAACCATGCCTACCCTGTGATGCAGTTTGGCAACATCTCTGCCTTCCACTGCAAC
  TACTGCCCCGAGATGTTCGCCGACATCAATAGCCTGCAGGAGCACATCCGCGTCTCCCACTGCGGCCCC
  AACGCCAACCCCTCTGACGGTAATAATGCTTTCTTCTGCAACCAGTGCTCCATGGGTTTCCTTACTGAG
  TCCTCCCTCACCGAGCACATCCAGCAGGCCCACTGCAGTGTGGGCAGTGCCAAACTAGAGTCTCCGGTG
  GTGCAGCCCACGCAGTCCTTCATGGAGGTCTATTCCTGCCCCTACTGCACCAACTCCCCCATCTTTGGC
  TCCATCCTGAAACTCACCAAGCACATCAAGGAGAACCACAAGAACATTCCACTGGCCCACAGCAAGAAG
  TCCAAGGCCGAGCAGAGCCCAGTCTCGTCCGATGTGGAGGTGTCTTCCCCGAAGCGGCAGCGGCTCTCA
  GCAAGCGCCAACTCCATCTCCAATGGGGAGTATCCTTGCAATCAATGCGACCTCAAGTTCTCCAACTTT
  GAGAGCTTCCAGACCCACCTGAAGCTGCACCTGGAGCTGCTGCTGCGGAAGCAAGCGTGCCCCCAGTGC
  AAAGAGGACTTTGACTCCCAGGAGTCCCTCCTGCAGCACCTGACAGTGCATTACATGACCACGTCGACC
  CACTATGTGTGCGAGAGCTGCGACAAGCAATTTTCCTCGGTGGATGACCTGCAGAAGCACCTGCTGGAC
  ATGCACACCTTTGTGTTGTACCACTGCACCCTGTGTCAGGAGGTCTTCGACTCCAAGGTGTCCATCCAG
  GTGCACCTGGCGGTGAAGCACAGCAATGAGAAGAAGATGTACCGCTGCACGGCCTGCAACTGGGACTTC
  CGCAAGGAGGCTGACCTGCAGGTGCACGTCAAACACAGCCACCTGGGCAACCCGGCCAAGGCTCACAAG
  TGCATCTTCTGTGGGGAGACCTTCAGCACCGAGGTGGAGCTGCAGTGCCACATCACCACACACAGCAAG
  AAGTATAACTGTAAGTTCTGCAGCAAGGCCTTCCACGCCATCATCCTGCTGGAGAAGCACCTGCGGGAG
  AAGCACTGTGTGTTTGATGCTGCGACCGAGAACGGCACGGCCAATGGGGTACCCCCAATGGCCACCAAG
  AAAGCTGAGCCTGCTGACCTGCAGGGCATGCTGCTTAAGAACCCTGAGGCACCTAACAGCCATGAGGCC
  AGCGAGGATGACGTGGACGCGTCGGAGCCCATGTACGGCTGTGACATCTGCGGGGCGGCCTACACCATG
  GAGGTGCTGCTGCAGAATCACCGGCTGCGGGACCACAATATCCGGCCGGGCGAGGATGATGGCTCACGC
  AAGAAGGCTGAGTTTATCAAGGGCAGTCACAAGTGCAACGTTTGTTCACGGACTTTCTTCTCGGAGAAC
  GGGCTACGGGAGCACCTGCAGACGCACCGGGGCCCTGCCAAGCACTACATGTGTCCCATCTGTGGTGAG
  CGCTTCCCTTCGCTGCTGACGCTCACCGAACACAAGGTGACCCACAGCAAGAGCCTGGACACGGGCACC
  TGTCGCATCTGCAAGATGCCCCTGCAGAGCGAGGAGGAGTTTATTGAGCACTGCCAGATGCACCCTGAC
  CTGCGCAACTCACTCACGGGCTTCCGCTGTGTGGTCTGCATGCAGACAGTCACTTCCACGCTTGAGCTC
  AAGATCCATGGCACCTTCCACATGCAGAAGCTGGCGGGCAGCTCAGCGGCGTCCTCCCCCAATGGCCAG
  GGGCTGCAGAAGCTCTACAAGTGCGCCCTGTGCCTCAAGGAGTTCCGCAGCAAGCAGGACCTGGTGAAG
  CTTGACGTCAATGGGCTGCCCTACGGCCTCTGCGCCGGCTGCATGGCCCGCAGCGCCAACGGACAGGTG
  GGTGGCCTGGCCCCGCCCGAGCCCGCCGACCGGCCCTGTGCCGGCCTCCGTTGCCCCGAGTGCAGTGTC
  AAGTTTGAGAGTGCCGAAGACCTGGAGAGCCACATGCAGGTGGACCACCGTGACCTCACGCCGGAGACC
  AGTGGGCCCCGGAAAGGCACCCAGACATCGCCAGTGCCCCGGAAAAAGACATACCAGTGCATCAAGTGC
  CAGATGACCTTCGAGAACGAGAGAGAGATCCAAATCCACGTTGCCAACCACATGATTGAGGAAGGCATC
  AACCACGAGTGTAAGCTGTGCAACCAGATGTTCGACTCCCCGGCCAAGCTCCTCTGTCACCTCATTGAG
  CACAGCTTCGAGGGCATGGGCGGCACCTTCAAATGCCCCGTGTGTTTCACAGTCTTCGTCCAGGCCAAC
  AAGTTGCAGCAGCACATCTTTGCCGTGCACGGGCAGGAGGACAAGATCTACGACTGCTCACAGTGCCCT
  CAGAAGTTCTTCTTCCAGACCGAGCTGCAGAACCACACGATGAGCCAGCACGCACAGAG
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by RC218703
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MHKKRVEEGEASDFSLAWDSSVTAAGGLEGEPECDQKTSRALEDRNSVTSQEERNEDDEDMEDESIYTC
  DHCQQDFESLADLTDHRAHRCPGDGDDDPQLSWVASSPSSKDVASPTQMIGDGCDLGLGEEEGGTGLPY
  PCQFCDKSFIRLSYLKRHEQIHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCHECEAAFSRSD
  HLKIHLKTHSSSKPFKCTVCKRGFSSTSSLQSHMQAHKKNKEHLAKSEKEAKKDDFMCDYCEDTFSQTE
  ELEKHVLTRHPQLSEKADLQCIHCPEVFVDENTLLAHIHQAHANQKHKCPMCPEQFSSVEGVYCHLDSH
  RQPDSSNHSVSPDPVLGSVASMSSATPDSSASVERGSTPDSTLKPLRGQKKMRDDGQGWTKVVYSCPYC
  SKRDFNSLAVLEIHLKTIHADKPQQSHTCQICLDSMPTLYNLNEHVRKLHKNHAYPVMQFGNISAFHCN
  YCPEMFADINSLQEHIRVSHCGPNANPSDGNNAFFCNQCSMGFLTESSLTEHIQQAHCSVGSAKLESPV
  VQPTQSFMEVYSCPYCTNSPIFGSILKLTKHIKENHKNIPLAHSKKSKAEQSPVSSDVEVSSPKRQRLS
  ASANSISNGEYPCNQCDLKFSNFESFQTHLKLHLELLLRKQACPQCKEDFDSQESLLQHLTVHYMTTST
  HYVCESCDKQFSSVDDLQKHLLDMHTFVLYHCTLCQEVFDSKVSIQVHLAVKHSNEKKMYRCTACNWDF
  RKEADLQVHVKHSHLGNPAKAHKCIFCGETFSTEVELQCHITTHSKKYNCKFCSKAFHAIILLEKHLRE
  KHCVFDAATENGTANGVPPMATKKAEPADLQGMLLKNPEAPNSHEASEDDVDASEPMYGCDICGAAYTM
  EVLLQNHRLRDHNIRPGEDDGSRKKAEFIKGSHKCNVCSRTFFSENGLREHLQTHRGPAKHYMCPICGE
  RFPSLLTLTEHKVTHSKSLDTGTCRICKMPLQSEEEFIEHCQMHPDLRNSLTGFRCVVCMQTVTSTLEL
  KIHGTFHMQKLAGSSAASSPNGQGLQKLYKCALCLKEFRSKQDLVKLDVNGLPYGLCAGCMARSANGQV
  GGLAPPEPADRPCAGLRCPECSVKFESAEDLESHMQVDHRDLTPETSGPRKGTQTSPVPRKKTYQCIKC
  QMTFENEREIQIHVANHMIEEGINHECKLCNQMFDSPAKLLCHLIEHSFEGMGGTFKCPVCFTVFVQAN
  KLQQHIFAVHGQEDKIYDCSQCPQKFFFQTELQNHTMSQHAQ
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015069
• ORF Size: 3861 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 4846 bp
• RefSeq ORF: 3855 bp
• Locus ID: 23090
• UniProt ID: Q2M1K9
• Cytogenetics: 16q12.1
• Domains: zf-C2H2
• MW: 144.8 kDa
• Gene Summary: The protein encoded by this gene is a nuclear protein that belongs to the family of Kruppel-like C2H2 zinc finger proteins. It functions as a DNA-binding transcription factor by using distinct zinc fingers in different signaling pathways. Thus, it is thought that this gene may have multiple roles in signal transduction during development. Mutations in this gene are associated with nephronophthisis-14 and Joubert syndrome-19. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2012]