ZC3H12B (NM_001010888) Human Tagged ORF Clone

CAT#: RC218677

ZC3H12B (Myc-DDK-tagged)-Human zinc finger CCCH-type containing 12B (ZC3H12B)

Product Images

Circular map for RC218677

• 

  Western blot validation of overexpression lysate (Cat# LY423209) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218677 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZC3H12B
• Synonyms: CXorf32; MCPIP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218677 representing NM_001010888
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGCCACAGCTGAGGTAGAGACACCAAAAATGGAGAAGAGTGCCTCCAAGGAAGAGAAGCAGCAGC
  CTAAGCAGGACAGCACAGAGCAGGGCAATGCTGATTCTGAAGAGTGGATGAGCTCTGAGAGTGACCCTGA
  ACAGATAAGCCTTAAGAGTAGCGACAACAGCAAGAGCTGCCAACCTAGGGATGGTCAGTTGAAGAAAAAG
  GAGATGCACTCCAAGCCACACCGCCAGCTCTGTCGATCACCCTGCCTAGATCGTCCAAGTTTCTCCCAGA
  GCAGCATTTTACAGGATGGTAAACTTGACTTGGAGAAGGAATACCAAGCTAAGATGGAGTTTGCGCTAAA
  GCTGGGCTATGCAGAGGAACAGATTCAATCAGTGCTAAACAAGCTGGGCCCAGAATCACTTATTAATGAT
  GTATTGGCAGAGCTTGTCAGACTTGGGAACAAAGGTGATTCAGAAGGGCAGATCAACTTGAGTCTGTTAG
  TGCCTCGTGGGCCCAGCTCCAGAGAGATTGCAAGCCCTGAATTGTCTCTTGAAGATGAAATAGATAACAG
  TGACAATTTGAGGCCAGTTGTCATTGATGGAAGTAATGTGGCAATGAGCCATGGGAATAAAGAAGAATTC
  TCCTGCAGAGGAATACAACTTGCTGTGGATTGGTTTCTAGATAAAGGCCATAAAGATATTACTGTATTTG
  TGCCTGCATGGAGAAAGGAGCAATCCCGCCCTGATGCACCAATTACAGATCAAGATATTCTACGAAAACT
  GGAGAAGGAAAAGATTCTTGTCTTCACACCATCCCGAAGAGTCCAAGGCAGGAGGGTTGTCTGCTATGAT
  GACCGGTTCATAGTCAAACTGGCTTTTGATTCTGATGGCATCATTGTGTCCAATGATAACTACCGAGACC
  TTCAAGTTGAAAAGCCAGAATGGAAGAAGTTTATAGAGGAGCGGTTGCTGATGTATTCTTTTGTGAATGA
  CAAATTTATGCCTCCAGATGATCCATTAGGACGCCACGGCCCAAGCCTTGAAAATTTCTTAAGAAAGAGA
  CCCATTGTTCCTGAGCATAAGAAGCAACCATGTCCTTATGGCAAAAAATGCACCTACGGCCACAAGTGCA
  AATACTACCATCCGGAGCGGGCCAACCAACCCCAGCGTTCGGTGGCTGATGAGCTCCGCATCAGTGCCAA
  ACTGTCCACAGTGAAAACTATGAGTGAAGGCACCCTGGCCAAGTGTGGCACAGGGATGTCTAGTGCCAAA
  GGTGAGATAACCTCAGAGGTCAAACGTGTGGCCCCCAAGCGCCAATCAGATCCCAGCATCCGGTCTGTGG
  CTATGGAGCCTGAGGAATGGCTGTCCATTGCCCGTAAGCCTGAGGCTAGTTCTGTCCCCTCGCTTGTGAC
  TGCCCTAAGTGTTCCCACAATCCCACCCCCCAAAAGCCATGCAGTGGGTGCACTCAACACCCGTTCGGCC
  AGCAGCCCAGTGCCAGGATCCTCCCATTTCCCCCACCAGAAGGCCTCTTTGGAGCATATGGCCAGCATGC
  AGTATCCTCCCATCTTGGTTACCAACAGCCATGGGACCCCTATTAGCTATGCTGAGCAATACCCAAAGTT
  TGAATCCATGGGGGACCATGGCTACTATTCAATGTTAGGTGACTTCTCCAAACTGAACATCAACAGCATG
  CATAACCGAGAGTATTACATGGCTGAAGTAGACCGGGGGGTGTATGCCCGGAATCCTAACCTCTGTTCTG
  ACAGCCGTGTGAGCCATACCAGGAATGACAACTATTCCTCTTACAACAACGTGTATTTGGCTGTAGCTGA
  TACCCATCCTGAAGGCAATTTGAAGCTGCACCGCTCAGCATCCCAGAACCGACTTCAGCCTTTTCCTCAT
  GGTTACCATGAAGCCTTAACACGAGTGCAGAGCTATGGCCCAGAGGATTCTAAGCAAGGCCCCCACAAAC
  AGTCAGTCCCCCACTTAGCTCTGCATGCCCAGCACCCATCAACTGGAACACGTTCCAGCTGTCCTGCAGA
  CTACCCCATGCCTCCCAATATCCATCCTGGGGCAACCCCCCAGCCAGGCCGTGCCCTGGTGATGACTCGG
  ATGGATAGCATTTCCGACTCCCGCCTCTATGAGAGCAACCCCGTGAGGCAAAGACGACCTCCCCTGTGCC
  GGGAACAGCATGCCAGCTGGGACCCGCTGCCCTGTACAACTGACTCCTATGGCTACCACTCCTATCCCTT
  GAGTAACAGCCTCATGCAACCATGTTATGAGCCAGTCATGGTACGGAGCGTGCCTGAAAAGATGGAGCAG
  CTTTGGAGGAATCCTTGGGTTGGAATGTGCAATGATTCCAGGGAGCATATGATCCCAGAACACCAGTATC
  AGACCTACAAGAACCTCTGCAATATTTTCCCTTCTAACATCGTCCTTGCAGTGATGGAGAAGAATCCCCA
  CACAGCAGATGCCCAGCAACTGGCAGCCTTGATTGTTGCTAAGCTTAGGGCTGCACGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218677 representing NM_001010888
  Red=Cloning site Green=Tags(s)
  
  MTATAEVETPKMEKSASKEEKQQPKQDSTEQGNADSEEWMSSESDPEQISLKSSDNSKSCQPRDGQLKKK
  EMHSKPHRQLCRSPCLDRPSFSQSSILQDGKLDLEKEYQAKMEFALKLGYAEEQIQSVLNKLGPESLIND
  VLAELVRLGNKGDSEGQINLSLLVPRGPSSREIASPELSLEDEIDNSDNLRPVVIDGSNVAMSHGNKEEF
  SCRGIQLAVDWFLDKGHKDITVFVPAWRKEQSRPDAPITDQDILRKLEKEKILVFTPSRRVQGRRVVCYD
  DRFIVKLAFDSDGIIVSNDNYRDLQVEKPEWKKFIEERLLMYSFVNDKFMPPDDPLGRHGPSLENFLRKR
  PIVPEHKKQPCPYGKKCTYGHKCKYYHPERANQPQRSVADELRISAKLSTVKTMSEGTLAKCGTGMSSAK
  GEITSEVKRVAPKRQSDPSIRSVAMEPEEWLSIARKPEASSVPSLVTALSVPTIPPPKSHAVGALNTRSA
  SSPVPGSSHFPHQKASLEHMASMQYPPILVTNSHGTPISYAEQYPKFESMGDHGYYSMLGDFSKLNINSM
  HNREYYMAEVDRGVYARNPNLCSDSRVSHTRNDNYSSYNNVYLAVADTHPEGNLKLHRSASQNRLQPFPH
  GYHEALTRVQSYGPEDSKQGPHKQSVPHLALHAQHPSTGTRSSCPADYPMPPNIHPGATPQPGRALVMTR
  MDSISDSRLYESNPVRQRRPPLCREQHASWDPLPCTTDSYGYHSYPLSNSLMQPCYEPVMVRSVPEKMEQ
  LWRNPWVGMCNDSREHMIPEHQYQTYKNLCNIFPSNIVLAVMEKNPHTADAQQLAALIVAKLRAAR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001010888
• ORF Size: 2508 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001010888.3, NP_001010888.3
• RefSeq Size: 7183 bp
• RefSeq ORF: 2511 bp
• Locus ID: 340554
• UniProt ID: Q5HYM0
• Cytogenetics: Xq11.2-q12
• MW: 94.21 kDa
• Gene Summary: The protein encoded by this gene belongs to a family of CCCH-type zinc finger proteins that are involved in the proinflammatory activation of macrophages. The exact function of this family member is unknown, but it is thought to function as a ribonuclease. [provided by RefSeq, May 2010]