PIP5K2 beta (PIP4K2B) (NM_003559) Human Tagged ORF Clone

CAT#: RC218338

PIP4K2B (Myc-DDK-tagged)-Human phosphatidylinositol-5-phosphate 4-kinase, type II, beta (PIP4K2B)

Product Images

Circular map for RC218338

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  Western blot validation of overexpression lysate (Cat# LY418600) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218338 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PIP4K2B protein (Cat# TP318338). The protein was produced from HEK293T cells transfected with PIP4K2B cDNA clone (Cat# RC218338) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PIP5K2 beta
• Synonyms: PI5P4KB; PIP5K2B; PIP5KIIB; PIP5KIIbeta; PIP5P4KB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218338 representing NM_003559
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCCAACTGCACCAGCACCACGGCGGTGGCGGTGGCGCCGCTCAGCGCCAGCAAGACCAAGACCA
  AGAAGAAGCATTTCGTGTGCCAGAAAGTGAAGCTATTCCGGGCCAGCGAGCCGATCCTCAGCGTCCTGAT
  GTGGGGGGTGAACCACACGATCAATGAGCTGAGCAATGTTCCTGTTCCTGTCATGCTAATGCCAGATGAC
  TTCAAAGCCTACAGCAAGATCAAGGTGGACAATCATCTCTTCAATAAGGAGAACCTGCCCAGCCGCTTTA
  AGTTTAAGGAGTATTGCCCCATGGTGTTCCGAAACCTTCGGGAGAGGTTTGGAATTGATGATCAGGATTA
  CCAGAATTCAGTGACGCGCAGCGCCCCCATCAACAGTGACAGCCAGGGTCGGTGTGGCACGCGTTTCCTC
  ACCACCTACGACCGGCGCTTTGTCATCAAGACTGTGTCCAGCGAGGACGTGGCGGAGATGCACAACATCT
  TAAAGAAATACCACCAGTTTATAGTGGAGTGTCATGGCAACACGCTTTTGCCACAGTTCCTGGGCATGTA
  CCGCCTGACCGTGGATGGTGTGGAAACCTACATGGTGGTTACCAGGAACGTGTTCAGCCATCGGCTCACT
  GTGCATCGCAAGTATGACCTCAAGGGTTCTACGGTTGCCAGAGAAGCGAGCGACAAGGAGAAGGCCAAGG
  ACTTGCCAACATTCAAAGACAATGACTTCCTCAATGAAGGGCAGAAGCTGCATGTGGGAGAGGAGAGTAA
  AAAGAACTTCCTGGAGAAACTGAAGCGGGACGTTGAGTTCTTGGCACAGCTGAAGATCATGGACTACAGC
  CTGCTGGTGGGCATCCACGACGTGGACCGGGCAGAGCAGGAGGAGATGGAGGTGGAGGAGCGGGCAGAGG
  ACGAGGAGTGTGAGAATGATGGGGTGGGTGGCAACCTACTCTGCTCCTATGGCACACCTCCGGACAGCCC
  TGGCAACCTCCTCAGCTTTCCTCGGTTCTTTGGTCCTGGGGAATTCGACCCCTCTGTTGACGTCTATGCC
  ATGAAAAGCCATGAAAGTTCCCCCAAGAAGGAGGTGTATTTCATGGCCATCATTGATATCCTCACGCCAT
  ACGATACAAAGAAGAAAGCTGCACATGCTGCCAAAACGGTGAAACACGGGGCAGGGGCCGAGATCTCGAC
  TGTGAACCCTGAGCAGTACTCCAAACGCTTCAACGAGTTTATGTCCAACATCCTGACG
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218338 representing NM_003559
  Red=Cloning site Green=Tags(s)
  
  MSSNCTSTTAVAVAPLSASKTKTKKKHFVCQKVKLFRASEPILSVLMWGVNHTINELSNVPVPVMLMPDD
  FKAYSKIKVDNHLFNKENLPSRFKFKEYCPMVFRNLRERFGIDDQDYQNSVTRSAPINSDSQGRCGTRFL
  TTYDRRFVIKTVSSEDVAEMHNILKKYHQFIVECHGNTLLPQFLGMYRLTVDGVETYMVVTRNVFSHRLT
  VHRKYDLKGSTVAREASDKEKAKDLPTFKDNDFLNEGQKLHVGEESKKNFLEKLKRDVEFLAQLKIMDYS
  LLVGIHDVDRAEQEEMEVEERAEDEECENDGVGGNLLCSYGTPPDSPGNLLSFPRFFGPGEFDPSVDVYA
  MKSHESSPKKEVYFMAIIDILTPYDTKKKAAHAAKTVKHGAGAEISTVNPEQYSKRFNEFMSNILT
  
  TRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003559
• ORF Size: 1248 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003559.4
• RefSeq Size: 3878 bp
• RefSeq ORF: 1251 bp
• Locus ID: 8396
• UniProt ID: P78356
• Cytogenetics: 17q12
• Domains: PIP5K
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system, Regulation of actin cytoskeleton
• MW: 47.2 kDa
• Gene Summary: The protein encoded by this gene catalyzes the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. The encoded protein sequence does not show similarity to other kinases, but the protein does exhibit kinase activity. Additionally, the encoded protein interacts with p55 TNF receptor. [provided by RefSeq, Jul 2008]