PIP5K2 beta (PIP4K2B) (NM_003559) Human Untagged Clone

CAT#: SC111707

PIP4K2B (untagged)-Human phosphatidylinositol-5-phosphate 4-kinase, type II, beta (PIP4K2B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIP5K2 beta
• Synonyms: PI5P4KB; PIP5K2B; PIP5KIIB; PIP5KIIbeta; PIP5P4KB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111707 sequence for NM_003559 edited (data generated by NextGen Sequencing)
  ATGTCGTCCAACTGCACCAGCACCACGGCGGTGGCGGTGGCGCCGCTCAGCGCCAGCAAG
  ACCAAGACCAAGAAGAAGCATTTCGTGTGCCAGAAAGTGAAGCTATTCCGGGCCAGCGAG
  CCGATCCTCAGCGTCCTGATGTGGGGGGTGAACCACACGATCAATGAGCTGAGCAATGTT
  CCTGTTCCTGTCATGCTAATGCCAGATGACTTCAAAGCCTACAGCAAGATCAAGGTGGAC
  AATCATCTCTTCAATAAGGAGAACCTGCCCAGCCGCTTTAAGTTTAAGGAGTATTGCCCC
  ATGGTGTTCCGAAACCTTCGGGAGAGGTTTGGAATTGATGATCAGGATTACCAGAATTCA
  GTGACGCGCAGCGCCCCCATCAACAGTGACAGCCAGGGTCGGTGTGGCACGCGTTTCCTC
  ACCACCTACGACCGGCGCTTTGTCATCAAGACTGTGTCCAGCGAGGACGTGGCGGAGATG
  CACAACATCTTAAAGAAATACCACCAGTTTATAGTGGAGTGTCATGGCAACACGCTTTTG
  CCACAGTTCCTGGGCATGTACCGCCTGACCGTGGATGGTGTGGAAACCTACATGGTGGTT
  ACCAGGAACGTGTTCAGCCATCGGCTCACTGTGCATCGCAAGTATGACCTCAAGGGTTCT
  ACGGTTGCCAGAGAAGCGAGCGACAAGGAGAAGGCCAAGGACTTGCCAACATTCAAAGAC
  AATGACTTCCTCAATGAAGGGCAGAAGCTGCATGTGGGAGAGGAGAGTAAAAAGAACTTC
  CTGGAGAAACTGAAGCGGGACGTTGAGTTCTTGGCACAGCTGAAGATCATGGACTACAGC
  CTGCTGGTGGGCATCCACGACGTGGACCGGGCAGAGCAGGAGGAGATGGAGGTGGAGGAG
  CGGGCAGAGGACGAGGAGTGTGAGAATGATGGGGTGGGTGGCAACCTACTCTGCTCCTAT
  GGCACACCTCCGGACAGCCCTGGCAACCTCCTCAGCTTTCCTCGGTTCTTTGGTCCTGGG
  GAATTCGACCCCTCTGTTGACGTCTATGCCATGAAAAGCCATGAAAGTTCCCCCAAGAAG
  GAGGTGTATTTCATGGCCATCATTGATATCCTCACGCCATACGATACAAAGAAGAAAGCT
  GCACATGCTGCCAAAACGGTGAAACACGGGGCAGGGGCCGAGATCTCGACTGTGAACCCT
  GAGCAGTACTCCAAACGCTTCAACGAGTTTATGTCCAACATCCTGACGTAG
  
  Clone variation with respect to NM_003559.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003559 unedited
  GGCGGCCCGCGAATTCGCACGAGGGCCGCTGGCTTGTGCGCTGTCTCCGCCGCCCCCCTC
  TTTCGCCGCCGCCGCCGCCGCCCCGGGCATGTCGTCCAACTGCACCAGCACCACGGCGGT
  GGCGGTGGCGCCGCTCAGCGCCAGCAAGACCAAGACCAAGAAGAAGCATTTCGTGTGCCA
  GAAAGTGAAGCTATTCCGGGCCAGCGAGCCGATCCTCAGCGTCCTGATGTGGGGGGTGAA
  CCACACGATCAATGAGCTGAGCAATGTTCCTGTTCCTGTCATGCTAATGCCAGATGACTT
  CAAAGCCTACAGCAAGATCAAGGTGGACAATCATCTCTTCAATAAGGAGAACCTGCCCAG
  CCGCTTTAAGTTTAAGGAGTATTGCCCCATGGTGTTCCGAAACCTTCGGGAGAGGTTTGG
  AATTGATGATCAGGATTACCAGAATTCAGTGACGCGCAGCGCCCCCATCAACAGTGACAG
  CCAGGGTCGGTGTGGCACGCGTTTCCTCACCACCTACGACCGGCGCTTTGTCATCAAGAC
  TGTGTCCAGCGAGGACGTGGCGGAGATGCACAACATCTTAAAGAAATACCACCAGTTTAT
  AGTGGAGTGTCATGGCAACACGCTTTTGCCACAGTTCCTGGGCATGTACCGCCTGACCGT
  GGATGGTGTGGAAACCTACATGGTGGTTACCAGGAACGTGTTCAGCCATCGGCTCACTGT
  GCATCGCAAGTATGACCTCAAGGGGTCTACGGTTGCCANAGAAGCGAGCGACAAGGAGAG
  GCCAAGGACTNGCCAACATTTCAAGACATGACTTNNCTCATGAGGGCANAAGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003559 unedited
  AGAGCTNAGGGGTCACAGGGCATGCCACCTCTTGTATCTGTTGCAGGAAAAGCTATGACC
  GCGGTCGCAAACTACAGTCGAGTTTTTTTTTTTTTTTTTTACACAAAGCTTTTTGAGTTT
  TATTTTTTTCCTTAGGTTGGAGATTCATCGTAACATGCATGCATTTTCAAACAGAAACCG
  GTACATAAATTTTAAAAAGCAGGCGTTTTTTTTGGGCGGGGGATTGAAAATTCCCATTTA
  AAAAATGGAAACTGCAGTGGGATATGGAGAAATCACACCCTTGGTGGGGTGGTGGTTTGT
  CCTGCTTCCCCAAAAGGTGGATATGAACTTAATTCACATTTTTTAATTTCAATGATCTGA
  CCTGATGCTGGGATGTGCCGAGCAGAACTGCCCCCTCCCGCAAGGGTGTTGGCTACCACC
  GCCTCTAACAGTGGCTTCTGCGCAAGGGCAAGAGGCTCCTGTGGAAACTTCTGCCCTGGG
  ATACTGGGACAATCTGGCAAGGGACAGCTGAGTCTCCTTTTGCCGTGGGGTACTCTTAAT
  AAGTGGTACATTCTTAAGGAGGGAGAGGGGCACGGGACCCTGACCCTCTGCATAGGGATG
  GTGGATTGGGGTAAAAATTGTCTGGTAGGGAGTTGTCCCTTACGGGGACGTCCCTGCCCT
  GCCTGATTGGTAGGAATGAAGGGAGGCCTCCTTGGTATTTGGGGAGGGCAATAGGTGTGA
  AGGGCTCGGAGAACATGAATTGTTATTTTAAAACCACAGCTGTGTTTTTTGTAAGCCTTC
  TATCTTCCTATTTATAACCGCCCGTGCGCTGGTGGTGTCCGGNGAAGTTCTGCTTGATAC
  CACGCTTAATNTTTGTTGTCAAAAATACCTACTCGAGGGAGAGTGTGTGGTGTGTATCTG
  TGATAGAGGGCCTGCTCGCGACAAGTATTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003559
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003559.3, NP_003550.1
• RefSeq Size: 3878 bp
• RefSeq ORF: 1251 bp
• Locus ID: 8396
• UniProt ID: P78356
• Cytogenetics: 17q12
• Domains: PIP5K
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system, Regulation of actin cytoskeleton
• Gene Summary: The protein encoded by this gene catalyzes the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. The encoded protein sequence does not show similarity to other kinases, but the protein does exhibit kinase activity. Additionally, the encoded protein interacts with p55 TNF receptor. [provided by RefSeq, Jul 2008]