ISLR2 (NM_020851) Human Tagged ORF Clone

CAT#: RC217963

ISLR2 (Myc-DDK-tagged)-Human immunoglobulin superfamily containing leucine-rich repeat 2 (ISLR2), transcript variant 2

Product Images

Circular map for RC217963

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ISLR2
• Synonyms: LINX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217963 representing NM_020851
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCCCCTTCGGGCCCTGTGGTTGGTCTGGGCGCTTCTAGGAGTGGCCGGATCATGCCCGGAGCCGT
  GCGCCTGCGTGGACAAGTACGCTCACCAGTTCGCGGACTGCGCTTACAAAGAGTTGCGTGAGGTGCCGGA
  AGGACTGCCTGCCAACGTGACGACGCTTAGTCTGTCCGCGAACAAGATCACTGTGCTGCGGCGCGGGGCC
  TTCGCCGACGTCACACAGGTCACGTCGCTGTGGCTGGCGCACAATGAGGTGCGCACCGTGGAGCCAGGCG
  CACTGGCCGTGCTGAGTCAGCTCAAGAACCTCGATCTGAGCCACAACTTCATATCCAGCTTTCCGTGGAG
  CGACCTGCGCAACCTGAGCGCGCTGCAGCTGCTCAAAATGAACCACAACCGCCTGGGCTCTCTGCCCCGG
  GACGCACTCGGTGCGCTACCCGACCTGCGTTCCCTGCGCATCAACAACAACCGGCTGCGTACGCTGGCGC
  CTGGCACCTTCGACGCGCTTAGCGCGCTGTCACACTTGCAACTCTATCACAATCCCTTCCACTGCGGCTG
  CGGCCTTGTGTGGCTGCAGGCCTGGGCCGCGAGCACCCGGGTGTCCTTACCCGAGCCCGACTCCATTGCT
  TGTGCCTCGCCTCCCGCGCTGCAGGGGGTGCCGGTGTACCGCCTGCCCGCCCTGCCCTGTGCACCGCCCA
  GCGTGCATCTGAGTGCCGAGCCACCGCTTGAAGCACCCGGCACCCCACTGCGCGCAGGACTGGCGTTCGT
  GTTACACTGCATCGCCGACGGCCACCCTACGCCTCGCCTGCAATGGCAACTTCAGATCCCCGGTGGCACC
  GTAGTCTTAGAGCCACCGGTTCTGAGCGGGGAGGACGACGGGGTTGGGGCGGAGGAAGGAGAGGGAGAAG
  GAGATGGGGATTTGCTGACGCAGACCCAAGCCCAAACGCCGACTCCAGCACCCGCTTGGCCGGCGCCCCC
  AGCCACACCGCGCTTCCTGGCCCTCGCAAATGGCTCCCTGTTGGTGCCCCTCCTGAGTGCCAAGGAGGCG
  GGCGTCTACACTTGCCGTGCACACAATGAGCTGGGCGCCAACTCTACGTCAATACGCGTGGCGGTGGCAG
  CAACCGGGCCCCCAAAACACGCGCCTGGCGCCGGGGGAGAACCCGACGGACAGGCCCCGACCTCTGAGCG
  CAAGTCCACAGCCAAGGGCCGGGGCAACAGCGTCCTGCCTTCCAAACCCGAGGGCAAAATCAAAGGCCAA
  GGCCTGGCCAAGGTCAGCATTCTCGGGGAGACCGAGACGGAGCCGGAGGAGGACACAAGTGAGGGAGAGG
  AGGCCGAAGACCAGATCCTCGCGGACCCGGCGGAGGAGCAGCGCTGTGGCAACGGGGACCCCTCTCGGTA
  CGTTTCTAACCACGCGTTCAACCAGAGCGCAGAGCTCAAGCCGCACGTCTTCGAGCTGGGCGTCATCGCG
  CTGGATGTGGCGGAGCGCGAGGCGCGGGTGCAGCTGACTCCGCTGGCTGCGCGCTGGGGCCCTGGGCCCG
  GCGGGGCTGGCGGAGCCCCGCGACCCGGGCGGCGACCCCTGCGCCTACTCTATCTGTGTCCAGCGGGGGG
  CGGCGCGGCAGTGCAGTGGTCCCGCGTAGAGGAAGGCGTCAACGCCTACTGGTTCCGCGGCCTGCGGCCG
  GGTACCAACTACTCCGTGTGCCTGGCGCTGGCGGGCGAAGCCTGCCACGTGCAAGTGGTGTTTTCCACCA
  AGAAGGAGCTCCCATCGCTGCTGGTCATAGTGGCAGTGAGCGTATTCCTCCTGGTGCTGGCCACAGTGCC
  CCTTCTGGGCGCCGCCTGCTGCCATCTGCTGGCTAAACACCCGGGCAAGCCCTACCGTCTGATCCTGCGG
  CCTCAGGCCCCTGACCCTATGGAGAAGCGCATCGCCGCAGACTTCGACCCGCGTGCTTCGTACCTCGAGT
  CCGAGAAAAGCTACCCGGCAGGCGGCGAGGCGGGCGGCGAGGAGCCAGAGGACGTGCAGGGGGAGGGCCT
  TGATGAAGACGCGGAGCAGGGAGACCCAAGTGGGGACCTGCAGAGAGAGGAGAGCCTGGCGGCCTGCTCA
  CTGGTGGAGTCCCAGTCCAAGGCCAACCAAGAGGAGTTCGAGGCGGGCTCTGAGTACAGCGATCGGCTGC
  CCCTGGGCGCCGAGGCGGTCAACATCGCCCAGGAGATTAATGGCAACTACAGGCAGACGGCAGGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217963 representing NM_020851
  Red=Cloning site Green=Tags(s)
  
  MFPLRALWLVWALLGVAGSCPEPCACVDKYAHQFADCAYKELREVPEGLPANVTTLSLSANKITVLRRGA
  FADVTQVTSLWLAHNEVRTVEPGALAVLSQLKNLDLSHNFISSFPWSDLRNLSALQLLKMNHNRLGSLPR
  DALGALPDLRSLRINNNRLRTLAPGTFDALSALSHLQLYHNPFHCGCGLVWLQAWAASTRVSLPEPDSIA
  CASPPALQGVPVYRLPALPCAPPSVHLSAEPPLEAPGTPLRAGLAFVLHCIADGHPTPRLQWQLQIPGGT
  VVLEPPVLSGEDDGVGAEEGEGEGDGDLLTQTQAQTPTPAPAWPAPPATPRFLALANGSLLVPLLSAKEA
  GVYTCRAHNELGANSTSIRVAVAATGPPKHAPGAGGEPDGQAPTSERKSTAKGRGNSVLPSKPEGKIKGQ
  GLAKVSILGETETEPEEDTSEGEEAEDQILADPAEEQRCGNGDPSRYVSNHAFNQSAELKPHVFELGVIA
  LDVAEREARVQLTPLAARWGPGPGGAGGAPRPGRRPLRLLYLCPAGGGAAVQWSRVEEGVNAYWFRGLRP
  GTNYSVCLALAGEACHVQVVFSTKKELPSLLVIVAVSVFLLVLATVPLLGAACCHLLAKHPGKPYRLILR
  PQAPDPMEKRIAADFDPRASYLESEKSYPAGGEAGGEEPEDVQGEGLDEDAEQGDPSGDLQREESLAACS
  LVESQSKANQEEFEAGSEYSDRLPLGAEAVNIAQEINGNYRQTAG
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020851
• ORF Size: 2235 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020851.3
• RefSeq Size: 4185 bp
• RefSeq ORF: 2238 bp
• Locus ID: 57611
• UniProt ID: Q6UXK2
• Cytogenetics: 15q24.1
• Protein Families: Druggable Genome, Transmembrane
• MW: 78.8 kDa
• Gene Summary: Required for axon extension during neural development.[UniProtKB/Swiss-Prot Function]