G0 Protein alpha(GNAO1) (NM_020988) Human Tagged ORF Clone

CAT#: RC217958

GNAO1 (Myc-DDK-tagged)-Human guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O (GNAO1), transcript variant 1

Product images

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Specifications

Product Data

• Product Name: G0 Protein alpha(GNAO1) (NM_020988) Human Tagged ORF Clone
• Symbol: GNAO1
• Synonyms: EIEE17; G-ALPHA-o; GNAO; HLA-DQB1
• Vector: pCMV6-Entry
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217958 representing NM_020988
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGATGTACTCTGAGCGCAGAGGAGAGAGCCGCCCTCGAGCGGAGCAAGGCGATTGAGAAAAACCTCA
  AAGAGGATGGCATCAGCGCCGCCAAAGACGTGAAATTACTCCTGCTCGGGGCTGGAGAATCAGGAAAAAG
  CACCATTGTGAAGCAGATGAAGATCATCCATGAAGATGGCTTCTCCGGAGAAGACGTGAAACAGTACAAG
  CCTGTTGTCTACAGCAACACTATCCAGTCCCTGGCAGCCATCGTCCGGGCCATGGACACTTTGGGCATCG
  AATATGGTGATAAGGAGAGAAAGGCTGACGCCAAGATGGTGTGTGATGTGGTGAGTCGGATGGAAGACAC
  CGAGCCCTTCTCTGCAGAGCTGCTTTCTGCCATGATGCGGCTCTGGGGCGACTCAGGAATCCAAGAGTGC
  TTCAACCGGTCCCGGGAGTATCAACTCAACGACTCTGCCAAATACTACCTGGACAGCCTGGATCGGATTG
  GGGCCGCCGACTACCAGCCCACTGAGCAGGACATCCTCCGAACCAGGGTCAAAACCACTGGCATCGTAGA
  AACCCACTTCACATTCAAGAACCTCCACTTCAGGCTGTTTGACGTCGGAGGCCAGCGATCTGAACGCAAG
  AAGTGGATCCATTGCTTCGAGGACGTCACGGCCATCATTTTCTGTGTCGCGCTCAGCGGCTATGACCAGG
  TGCTCCACGAAGACGAAACCACGAACCGCATGCACGAGTCTCTCATGCTCTTCGACTCCATCTGTAACAA
  CAAGTTCTTCATCGATACCTCCATCATTCTCTTCCTCAACAAGAAAGATCTCTTTGGCGAGAAGATCAAG
  AAGTCACCTTTGACCATCTGCTTTCCTGAATACACAGGCCCCAATACCTATGAAGACGCAGCCGCCTACA
  TCCAAGCACAATTTGAAAGCAAAAACCGCTCACCCAACAAAGAAATATATTGTCACATGACTTGTGCCAC
  AGACACGAATAACATCCAGGTGGTGTTCGACGCCGTCACCGACATCATCATTGCCAACAACCTCCGGGGC
  TGCGGCTTGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217958 representing NM_020988
  Red=Cloning site Green=Tags(s)
  
  MGCTLSAEERAALERSKAIEKNLKEDGISAAKDVKLLLLGAGESGKSTIVKQMKIIHEDGFSGEDVKQYK
  PVVYSNTIQSLAAIVRAMDTLGIEYGDKERKADAKMVCDVVSRMEDTEPFSAELLSAMMRLWGDSGIQEC
  FNRSREYQLNDSAKYYLDSLDRIGAADYQPTEQDILRTRVKTTGIVETHFTFKNLHFRLFDVGGQRSERK
  KWIHCFEDVTAIIFCVALSGYDQVLHEDETTNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFGEKIK
  KSPLTICFPEYTGPNTYEDAAAYIQAQFESKNRSPNKEIYCHMTCATDTNNIQVVFDAVTDIIIANNLRG
  CGLY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• Tag: Myc-DDK
• ACCN: NM_020988
• ORF Size: 1062 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_020988.1, NM_020988.2, NP_066268
• RefSeq Size: 3332
• RefSeq ORF: 1065
• Locus ID: 2775
• Cytogenetics: 16q13
• Domains: G-alpha
• Protein Families: Druggable Genome
• Protein Pathways: Long-term depression, Melanogenesis
• MW: 40.1 kDa
• Gene Summary: The protein encoded by this gene represents the alpha subunit of the Go heterotrimeric G-protein signal-transducing complex. Defects in this gene are a cause of early-onset epileptic encephalopathy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015].