Melatonin Related Receptor (GPR50) (NM_004224) Human Tagged ORF Clone

CAT#: RC217839

GPR50 (Myc-DDK-tagged)-Human G protein-coupled receptor 50 (GPR50)

Product Images

Circular map for RC217839

• 

  Western blot validation of overexpression lysate (Cat# LY401354) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217839 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Melatonin Related Receptor
• Synonyms: H9; Mel1c
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217839 representing NM_004224
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCCACCCTAGCGGTTCCCACCCCCTATGGCTGTATTGGCTGTAAGCTACCCCAGCCAGAATACC
  CACCGGCTCTAATCATCTTTATGTTCTGCGCGATGGTTATCACCATCGTTGTAGACCTAATCGGCAACTC
  CATGGTCATTTTGGCTGTGACGAAGAACAAGAAGCTCCGGAATTCTGGCAACATCTTCGTAGTCAGTCTC
  TCTGTGGCCGATATGCTGGTGGCCATCTACCCATACCCTTTGATGCTGCATGCCATGTCCATTGGGGGCT
  GGGATCTGAGCCAGTTACAGTGCCAGATGGTCGGGTTCATCACAGGGCTGAGTGTGGTCGGCTCCATCTT
  CAACATCGTGGCAATCGCTATCAACCGTTACTGCTACATCTGCCACAGCCTCCAGTACGAACGGATCTTC
  AGTGTGCGCAATACCTGCATCTACCTGGTCATCACCTGGATCATGACCGTCCTGGCTGTCCTGCCCAACA
  TGTACATTGGCACCATCGAGTACGATCCTCGCACCTACACCTGCATCTTCAACTATCTGAACAACCCTGT
  CTTCACTGTTACCATCGTCTGCATCCGCTTCGTCCTCCCTCTCCTCATCGTGGGTTTCTGCTACGTGAGG
  ATCTGGACCAAAGTGCTGGCGGCCCGTGACCCTGCAGGGCAGAATCCTGACAACCAACTTGCTGAGGTTC
  GCAATTTTCTAACCATGTTTGTGATCTTCCTCCTCTTTGCAGTGTGCTGGTGCCCTATCAACGTGCTCAC
  TGTCTTGGTGGCTGTCAGTCCGAAGGAGATGGCAGGCAAGACCCCCAACTGGCTTTATCTTGCAGCCTAC
  TTCATAGCCTACTTCAACAGCTGCCTCAACGCTGTGATCTACGGGCTCCTCAATGAGAATTTCCGAAGAG
  AATACTGGACCATCTTCCATGCTATGCGGCACCCTATCATATTCTTCTCTGGCCTCATCAGTGATATTCG
  TGAGATGCAGGAGGCCCGTACCCTGGCCCGCGCCCGTGCCTATGCTCGCGACCAAGCTCGTGAACAAGAC
  CGTGCCCATGCCTGTCCTGCTGTGGAGGAAACCCCGATGAATGTCCGGAATGTTCCATTACCTGGTGATG
  CTGCAGCTGGCCACCCCGACCGTGCCTCTGGCCACCCTAAGCCCCATTCCAGATCCTCCTCTGCCTATCG
  CAAATCTGCCTCTACCCACCACAAGTCTGTCTTTAGCCACTCCAAGGCTGCCTCTGGTCACCTCAAGCCT
  GTCTCTGGCCACTCCAAGCCTGCCTCTGGTCACCCCAAGTCTGCCACTGTCTACCCTAAGCCTGCCTCTG
  TCCATTTCAAGGCTGACTCTGTCCATTTCAAGGGTGACTCTGTCCATTTCAAGCCTGACTCTGTTCATTT
  CAAGCCTGCTTCCAGCAACCCCAAGCCCATCACTGGCCACCATGTCTCTGCTGGCAGCCACTCCAAGTCT
  GCCTTCAGTGCTGCCACCAGCCACCCTAAACCCACCACTGGCCACATCAAGCCAGCTACCAGCCATGCTG
  AGCCCACCACTGCTGACTATCCCAAGCCTGCCACTACCAGCCACCCTAAGCCCGCTGCTGCTGACAACCC
  TGAGCTCTCTGCCTCCCATTGCCCCGAGATCCCTGCCATTGCCCACCCTGTGTCTGACGACAGTGACCTC
  CCTGAGTCGGCCTCTAGCCCTGCCGCTGGGCCCACCAAGCCTGCTGCCAGCCAGCTGGAGTCTGACACCA
  TCGCTGACCTTCCTGACCCTACTGTAGTCACTACCAGTACCAATGATTACCATGATGTCGTGGTTGTTGA
  TGTTGAAGATGATCCTGATGAAATGGCTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217839 representing NM_004224
  Red=Cloning site Green=Tags(s)
  
  MGPTLAVPTPYGCIGCKLPQPEYPPALIIFMFCAMVITIVVDLIGNSMVILAVTKNKKLRNSGNIFVVSL
  SVADMLVAIYPYPLMLHAMSIGGWDLSQLQCQMVGFITGLSVVGSIFNIVAIAINRYCYICHSLQYERIF
  SVRNTCIYLVITWIMTVLAVLPNMYIGTIEYDPRTYTCIFNYLNNPVFTVTIVCIRFVLPLLIVGFCYVR
  IWTKVLAARDPAGQNPDNQLAEVRNFLTMFVIFLLFAVCWCPINVLTVLVAVSPKEMAGKTPNWLYLAAY
  FIAYFNSCLNAVIYGLLNENFRREYWTIFHAMRHPIIFFSGLISDIREMQEARTLARARAYARDQAREQD
  RAHACPAVEETPMNVRNVPLPGDAAAGHPDRASGHPKPHSRSSSAYRKSASTHHKSVFSHSKAASGHLKP
  VSGHSKPASGHPKSATVYPKPASVHFKADSVHFKGDSVHFKPDSVHFKPASSNPKPITGHHVSAGSHSKS
  AFSAATSHPKPTTGHIKPATSHAEPTTADYPKPATTSHPKPAAADNPELSASHCPEIPAIAHPVSDDSDL
  PESASSPAAGPTKPAASQLESDTIADLPDPTVVTTSTNDYHDVVVVDVEDDPDEMAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004224
• ORF Size: 1851 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004224.3
• RefSeq Size: 2020 bp
• RefSeq ORF: 1854 bp
• Locus ID: 9248
• UniProt ID: Q13585
• Cytogenetics: Xq28
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 67.4 kDa
• Gene Summary: This gene product belongs to the G-protein coupled receptor 1 family. Even though this protein shares similarity with the melatonin receptors, it does not bind melatonin, however, it inhibits melatonin receptor 1A function through heterodimerization. Polymorphic variants of this gene have been associated with bipolar affective disorder in women. [provided by RefSeq, Jan 2010]