Melatonin Related Receptor (GPR50) (NM_004224) Human Untagged Clone

CAT#: SC318061

GPR50 (untagged)-Human G protein-coupled receptor 50 (GPR50)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Melatonin Related Receptor
• Synonyms: H9; Mel1c
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004224 edited
  TTAACGATCCCCAGGAGCAACATGGGGCCCACCCTAGCGGTTCCCACCCCCTATGGCTGT
  ATTGGCTGTAAGCTACCCCAGCCAGAATACCCACCGGCTCTAATCATCTTTATGTTCTGC
  GCGATGGTTATCACCATCGTTGTAGACCTAATCGGCAACTCCATGGTCATTTTGGCTGTG
  ACGAAGAACAAGAAGCTCCGGAATTCTGGCAACATCTTCGTAGTCAGTCTCTCTGTGGCC
  GATATGCTGGTGGCCATCTACCCATACCCTTTGATGCTGCATGCCATGTCCATTGGGGGC
  TGGGATCTGAGCCAGTTACAGTGCCAGATGGTCGGGTTCATCACAGGGCTGAGTGTGGTC
  GGCTCCATCTTCAACATCGTGGCAATCGCTATCAACCGTTACTGCTACATCTGCCACAGC
  CTCCAGTACGAACGGATCTTCAGTGTGCGCAATACCTGCATCTACCTGGTCATCACCTGG
  ATCATGACCGTCCTGGCTGTCCTGCCCAACATGTACATTGGCACCATCGAGTACGATCCT
  CGCACCTACACCTGCATCTTCAACTATCTGAACAACCCTGTCTTCACTGTTACCATCGTC
  TGCATCCGCTTCGTCCTCCCTCTCCTCATCGTGGGTTTCTGCTACGTGAGGATCTGGACC
  AAAGTGCTGGCGGCCCGTGACCCTGCAGGGCAGAATCCTGACAACCAACTTGCTGAGGTT
  CGCAATTTTCTAACCATGTTTGTGATCTTCCTCCTCTTTGCAGTGTGCTGGTGCCCTATC
  AACGTGCTCACTGTCTTGGTGGCTGTCAGTCCGAAGGAGATGGCAGGCAAGACCCCCAAC
  TGGCTTTATCTTGCAGCCTACTTCATAGCCTACTTCAACAGCTGCCTCAACGCTGTGATC
  TACGGGCTCCTCAATGAGAATTTCCGAAGAGAATACTGGACCATCTTCCATGCTATGCGG
  CACCCTATCATATTCTTCTCTGGCCTCATCAGTGATATTCGTGAGATGCAGGAGGCCCGT
  ACCCTGGCCCGCGCCCGTGCCTATGCTCGCGACCAAGCTCGTGAACAAGACCGTGCCCAT
  GCCTGTCCTGCTGTGGAGGAAACCCCGATGAATGTCCGGAATGTTCCATTACCTGGTGAT
  GCTGCAGCTGGCCACCCCGACCGTGCCTCTGGCCACCCTAAGCCCCATTCCAGATCCTCC
  TCTGCCTATCGCAAATCTGCCTCTACCCACCACAAGTCTGTCTTTAGCCACTCCAAGGCT
  GCCTCTGGTCACCTCAAGCCTGTCTCTGGCCACTCCAAGCCTGCCTCTGGTCACCCCAAG
  TCTGCCACTGTCTACCCTAAGCCTGCCTCTGTCCATTTCAAGGCTGACTCTGTCCATTTC
  AAGGGTGACTCTGTCCATTTCAAGCCTGACTCTGTTCATTTCAAGCCTGCTTCCAGCAAC
  CCCAAGCCCATCACTGGCCACCATGTCTCTGCTGGCAGCCACTCCAAGTCTGCCTTCAGT
  GCTGCCACCAGCCACCCTAAACCCACCACTGGCCACATCAAGCCAGCTACCAGCCATGCT
  GAGCCCACCACTGCTGACTATCCCAAGCCTGCCACTACCAGCCACCCTAAGCCCGCTGCT
  GCTGACAACCCTGAGCTCTCTGCCTCCCATTGCCCCGAGATCCCTGCCATTGCCCACCCT
  GTGTCTGACGACAGTGACCTCCCTGAGTCGGCCTCTAGCCCTGCCGCTGGGCCCACCAAG
  CCTGCTGCCAGCCAGCTGGAGTCTGACACCATCGCTGACCTTCCTGACCCTACTGTAGTC
  ACTACCAGTACCAATGATTACCATGATGTCGTGGTTGTTGATGTTGAAGATGATCCTGAT
  GAAATGGCTGTGTGAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004224 unedited
  CACATTTTGTNTNNAACTCACTATAGGGCGGCCGCGAATTCAGATCTGGTACCGAGCTCG
  GATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAACGATCCCCAGGAGCAA
  CATGGGGCCCACCCTAGCGGTTCCCACCCCCTATGGCTGTATTGGCTGTAAGCTACCCCA
  GCCAGAATACCCACCGGCTCTAATCATCTTTATGTTCTGCGCGATGGTTATCACCATCGT
  TGTAGACCTAATCGGCAACTCCATGGTCATTTTGGCTGTGACGAAGAACAAGAAGCTCCG
  GAATTCTGGCAACATCTTCGTAGTCAGTCTCTCTGTGGCCGATATGCTGGTGGCCATCTA
  CCCATACCCTTTGATGCTGCATGCCATGTCCATTGGGGGCTGGGATCTGAGCCAGTTACA
  GTGCCAGATGGTCGGGTTCATCACAGGGCTGAGTGTGGTCGGCTCCATCTTCAACATCGT
  GGCAATCGCTATCAACCGTTACTGCTACATCTGCCACAGCCTCCAGTACGAACGGATCTT
  CAGTGTGCGCAATAACTGCATCTACCTGGTCATCACCTGGATCATGACCGGTCTGGCTGT
  CCTTGCCAACATGGTACATTGGACCCTTTCAATTAGAATCTTGGAAACTAACACGTGGAT
  TTCAATTATTTAAACACCCTGGTCTCCCTGGAACCAACGTCGGAACCGTTTTCCCCTCCT
  TCCCTAATCGGGGGTTCCGCCACGTGAAGTTCGGACCAAGG
  
• Restriction Sites: Please inquire
• ACCN: NM_004224
• Insert Size: 1900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004224.2, NP_004215.2
• RefSeq Size: 2010 bp
• RefSeq ORF: 1854 bp
• Locus ID: 9248
• UniProt ID: Q13585
• Cytogenetics: Xq28
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: This gene product belongs to the G-protein coupled receptor 1 family. Even though this protein shares similarity with the melatonin receptors, it does not bind melatonin, however, it inhibits melatonin receptor 1A function through heterodimerization. Polymorphic variants of this gene have been associated with bipolar affective disorder in women. [provided by RefSeq, Jan 2010]