PFAS (NM_012393) Human Tagged ORF Clone

CAT#: RC217795

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  • TrueORF®

PFAS (Myc-DDK-tagged)-Human phosphoribosylformylglycinamidine synthase (PFAS)



  "NM_012393" in other vectors (4)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 1,587.00


Availability*
4 Weeks

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PFAS
Synonyms FGAMS; FGAR-AT; FGARAT; GATD8; PURL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC217795 representing NM_012393
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCCAGTCCTTCACTTCTATGTTCGTCCCTCTGGCCATGAGGGGGCAGCCCCTGGACACACTCGGA
GGAAACTGCAAGGGAAACTGCCAGAGCTGCAGGGCGTCGAGACTGAACTGTGCTACAACGTGAACTGGAC
AGCTGAGGCCCTCCCCAGTGCTGAGGAGACAAAGAAGCTGATGTGGCTGTTTGGTTGCCCCTTACTGCTG
GATGATGTTGCTCGGGAGTCCTGGCTCCTTCCTGGCTCCAATGACCTGCTGCTGGAGGTCGGGCCCAGGC
TGAACTTCTCCACCCCAACATCCACCAACATCGTGTCAGTGTGCCGCGCCACTGGGCTGGGGCCTGTGGA
TCGTGTGGAGACCACCCGGCGCTACCGGCTCTCGTTTGCCCACCCCCCGTCAGCTGAGGTGGAAGCCATT
GCTCTGGCTACCCTGCACGACCGGATGACAGAGCAGCACTTCCCCCATCCCATCCAGAGTTTCTCCCCTG
AGAGCATGCCGGAACCCCTCAATGGCCCTATCAATATACTGGGTGAGGGCCGGCTTGCGCTGGAGAAGGC
CAACCAGGAGCTTGGTCTGGCTTTAGACTCTTGGGACCTAGACTTCTACACCAAGCGCTTCCAGGAGCTA
CAGCGGAACCCGAGCACTGTGGAGGCCTTTGACTTGGCGCAGTCCAATAGCGAGCACAGCCGACACTGGT
TCTTCAAGGGCCAGCTCCACGTGGATGGGCAGAAGCTGGTGCACTCACTGTTTGAGTCCATCATGAGCAC
CCAGGAATCCTCGAACCCCAACAACGTCCTCAAATTCTGTGATAACAGCAGTGCAATCCAGGGAAAGGAA
GTCCGATTCCTACGGCCTGAGGACCCCACACGGCCAAGCCGCTTCCAGCAACAGCAAGGGCTGAGACATG
TTGTCTTCACAGCAGAGACTCACAACTTTCCCACAGGAGTATGCCCCTTTAGTGGTGCAACCACTGGCAC
AGGGGGCCGGATTCGAGATGTCCAGTGCACAGGCCGCGGGGCCCACGTGGTGGCTGGCACTGCCGGCTAT
TGCTTTGGAAATCTGCATATTCCAGGTTACAATCTGCCCTGGGAGGATCCAAGCTTCCAGTATCCTGGGA
ATTTTGCCCGGCCCCTGGAGGTTGCCATTGAAGCCAGTAATGGAGCTTCTGACTATGGCAACAAGTTTGG
GGAACCAGTGCTGGCTGGCTTCGCCCGCTCCTTGGGCCTCCAGCTCCCAGACGGCCAGCGGCGTGAGTGG
ATCAAGCCCATCATGTTTAGTGGGGGCATTGGGTCCATGGAAGCTGACCACATAAGCAAGGAGGCCCCAG
AGCCAGGCATGGAAGTTGTAAAGGTTGGAGGTCCCGTCTACAGGATTGGAGTTGGAGGTGGAGCTGCTTC
ATCTGTGCAGGTGCAGGGAGATAACACCAGTGACCTGGACTTTGGGGCTGTGCAGCGAGGAGACCCGGAG
ATGGAACAGAAGATGAACCGTGTGATCAGGGCTTGTGTGGAGGCCCCCAAGGGAAACCCCATCTGCAGCC
TTCATGATCAGGGCGCTGGTGGCAATGGCAATGTCCTAAAAGAGCTGAGTGACCCAGCTGGAGCCATCAT
TTACACCAGCCGCTTCCAGCTTGGGGACCCAACCCTGAATGCCCTGGAAATCTGGGGGGCTGAGTACCAG
GAATCAAATGCTCTTCTGCTGAGGTCCCCCAACCGGGACTTCCTGACTCATGTCAGTGCCCGTGAACGTT
GCCCGGCTTGCTTCGTGGGCACCATCACTGGAGACCGGAGAATAGTGCTGGTGGACGATCGGGAGTGTCC
TGTCAGAAGAAATGGCCAGGGGGATGCCCCCCCGACACCCCTGCCAACCCCTGTGGACCTGGAGCTCGAA
TGGGTGCTGGGCAAGATGCCTCGGAAGGAGTTCTTCCTGCAGAGGAAGCCCCCCATGCTGCAGCCTCTGG
CCTTGCCCCCAGGGCTGAGCGTGCACCAGGCTCTGGAGAGGGTTCTGAGGCTGCCCGCCGTGGCCAGCAA
GCGCTACCTCACCAATAAGGTGGACCGCTCTGTGGGAGGCCTGGTGGCCCAGCAGCAGTGCGTGGGGCCC
CTGCAAACTCCTCTGGCAGATGTAGCGGTTGTGGCACTGAGCCATGAGGAGCTCATAGGGGCTGCCACAG
CCTTGGGAGAACAGCCAGTCAAGAGCCTGCTGGACCCAAAAGTCGCCGCCCGGCTGGCCGTGGCCGAAGC
CCTCACCAACCTGGTGTTTGCTCTGGTCACTGACCTCCGGGATGTGAAGTGTAGCGGGAACTGGATGTGG
GCAGCCAAGCTCCCAGGGGAGGGCGCAGCTTTGGCGGATGCCTGTGAGGCTATGGTGGCAGTGATGGCAG
CCCTGGGTGTGGCAGTGGATGGTGGCAAGGACTCCCTCAGCATGGCTGCTCGGGTTGGCACTGAGACCGT
GCGGGCTCCTGGGTCACTGGTCATCTCAGCCTATGCCGTCTGCCCAGACATCACAGCCACTGTGACCCCA
GACCTCAAGCATCCTGAAGGGAGAGGCCATCTGCTCTATGTGGCTCTGAGCCCTGGGCAGCACCGGCTCG
GGGGCACAGCTCTGGCCCAGTGCTTCTCCCAGCTTGGGGAACACCCTCCAGACCTGGACCTTCCTGAGAA
CTTGGTGCGGGCCTTCAGCATCACTCAGGGGCTGCTGAAAGACCGCCTCCTCTGCTCAGGCCACGATGTC
AGTGACGGAGGCCTCGTCACATGCCTGCTGGAGATGGCCTTTGCTGGAAATTGCGGGCTACAGGTGGATG
TGCCTGTCCCCAGGGTTGATGTCCTGTCTGTGCTGTTCGCTGAGGAGCCAGGCCTCGTGCTGGAGGTGCA
GGAGCCAGACCTGGCCCAGGTGCTGAAGCGTTACCGGGATGCTGGCCTCCATTGCCTGGAGCTGGGCCAC
ACAGGCGAGGCCGGGCCCCACGCCATGGTCCGGGTGTCAGTGAACGGGGCTGTGGTTCTGGAGGAGCCTG
TTGGGGAGCTGCGAGCCCTCTGGGAGGAGACGAGTTTCCAGCTGGACCGGCTACAGGCAGAGCCTCGCTG
TGTGGCAGAGGAGGAACGGGGCCTGAGGGAGCGGATGGGGCCCAGCTATTGCCTGCCCCCCACCTTTCCC
AAAGCCTCCGTGCCCCGTGAGCCTGGTGGTCCCAGCCCCCGAGTCGCCATCTTGCGAGAGGAGGGCAGTA
ATGGAGACCGGGAGATGGCCGATGCCTTCCACTTAGCTGGGTTTGAGGTATGGGACGTGACCATGCAGGA
CCTCTGCTCTGGGGCAATTGGGCTGGACACTTTCCGTGGCGTGGCCTTCGTGGGCGGCTTCAGCTATGCA
GATGTCCTGGGCTCTGCCAAAGGGTGGGCAGCTGCTGTGACCTTTCATCCCAGGGCTGGGGCTGAGCTGA
GGCGCTTCCGGAAGCGGCCAGACACCTTCAGCCTGGGCGTGTGTAATGGCTGTCAACTGCTGGCTCTGCT
CGGCTGGGTGGGAGGCGACCCCAATGAGGATGCTGCAGAGATGGGCCCTGACTCCCAGCCAGCCCGGCCA
GGCCTTCTGCTACGCCACAACCTGTCTGGGCGCTACGAGTCTCGCTGGGCCAGCGTGCGTGTGGGGCCTG
GGCCAGCCCTGATGCTGCGAGGGATGGAGGGCGCCGTGCTGCCCGTGTGGAGTGCGCACGGGGAAGGTTA
CGTAGCATTTTCTTCTCCGGAACTCCAAGCTCAGATTGAGGCCAGGGGCTTGGCTCCACTGCACTGGGCT
GATGATGACGGGAACCCCACAGAGCAGTACCCTCTGAATCCCAATGGGTCCCCAGGGGGCGTGGCTGGCA
TCTGCTCCTGTGATGGCCGCCACCTGGCTGTCATGCCTCACCCTGAGCGGGCCGTTAGGCCTTGGCAGTG
GGCATGGCGACCCCCTCCATTTGATACTCTGACCACCTCCCCCTGGCTCCAGCTCTTTATCAATGCCCGA
AACTGGACCCTGGAAGGGAGCTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC217795 representing NM_012393
Red=Cloning site Green=Tags(s)

MSPVLHFYVRPSGHEGAAPGHTRRKLQGKLPELQGVETELCYNVNWTAEALPSAEETKKLMWLFGCPLLL
DDVARESWLLPGSNDLLLEVGPRLNFSTPTSTNIVSVCRATGLGPVDRVETTRRYRLSFAHPPSAEVEAI
ALATLHDRMTEQHFPHPIQSFSPESMPEPLNGPINILGEGRLALEKANQELGLALDSWDLDFYTKRFQEL
QRNPSTVEAFDLAQSNSEHSRHWFFKGQLHVDGQKLVHSLFESIMSTQESSNPNNVLKFCDNSSAIQGKE
VRFLRPEDPTRPSRFQQQQGLRHVVFTAETHNFPTGVCPFSGATTGTGGRIRDVQCTGRGAHVVAGTAGY
CFGNLHIPGYNLPWEDPSFQYPGNFARPLEVAIEASNGASDYGNKFGEPVLAGFARSLGLQLPDGQRREW
IKPIMFSGGIGSMEADHISKEAPEPGMEVVKVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPE
MEQKMNRVIRACVEAPKGNPICSLHDQGAGGNGNVLKELSDPAGAIIYTSRFQLGDPTLNALEIWGAEYQ
ESNALLLRSPNRDFLTHVSARERCPACFVGTITGDRRIVLVDDRECPVRRNGQGDAPPTPLPTPVDLELE
WVLGKMPRKEFFLQRKPPMLQPLALPPGLSVHQALERVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGP
LQTPLADVAVVALSHEELIGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLRDVKCSGNWMW
AAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGTETVRAPGSLVISAYAVCPDITATVTP
DLKHPEGRGHLLYVALSPGQHRLGGTALAQCFSQLGEHPPDLDLPENLVRAFSITQGLLKDRLLCSGHDV
SDGGLVTCLLEMAFAGNCGLQVDVPVPRVDVLSVLFAEEPGLVLEVQEPDLAQVLKRYRDAGLHCLELGH
TGEAGPHAMVRVSVNGAVVLEEPVGELRALWEETSFQLDRLQAEPRCVAEEERGLRERMGPSYCLPPTFP
KASVPREPGGPSPRVAILREEGSNGDREMADAFHLAGFEVWDVTMQDLCSGAIGLDTFRGVAFVGGFSYA
DVLGSAKGWAAAVTFHPRAGAELRRFRKRPDTFSLGVCNGCQLLALLGWVGGDPNEDAAEMGPDSQPARP
GLLLRHNLSGRYESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYVAFSSPELQAQIEARGLAPLHWA
DDDGNPTEQYPLNPNGSPGGVAGICSCDGRHLAVMPHPERAVRPWQWAWRPPPFDTLTTSPWLQLFINAR
NWTLEGSC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_012393
ORF Size 4014 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_012393.3
RefSeq Size 5338 bp
RefSeq ORF 4017 bp
Locus ID 5198
UniProt ID O15067
Cytogenetics 17p13.1
Protein Pathways Metabolic pathways, Purine metabolism
MW 144.6 kDa
Gene Summary Purines are necessary for many cellular processes, including DNA replication, transcription, and energy metabolism. Ten enzymatic steps are required to synthesize inosine monophosphate (IMP) in the de novo pathway of purine biosynthesis. The enzyme encoded by this gene catalyzes the fourth step of IMP biosynthesis. [provided by RefSeq, Jul 2008]

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