Chloride Channel 5 (CLCN5) (NM_000084) Human Tagged ORF Clone

CAT#: RC217750

CLCN5 (Myc-DDK-tagged)-Human chloride channel 5 (CLCN5), transcript variant 3

Product Images

Circular map for RC217750

• 

  Western blot validation of overexpression lysate (Cat# LY400025) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217750 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chloride Channel 5
• Synonyms: ClC-5; CLC5; CLCK2; DENT1; DENTS; hCIC-K2; NPHL1; NPHL2; XLRH; XRN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217750 representing NM_000084.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGACTTCTTGGAGGAGCCAATCCCTGGTGTAGGGACCTATGATGATTTCAATACAATTGATTGGGTG
  AGAGAGAAGTCTCGAGACCGGGATAGGCACCGAGAGATTACCAATAAAAGCAAAGAGTCAACATGGGCC
  TTAATTCACAGTGTGAGTGATGCTTTTTCCGGCTGGTTGTTGATGCTCCTTATTGGGCTTTTATCAGGT
  TCGTTAGCTGGTTTGATAGACATCTCTGCTCATTGGATGACAGACTTAAAAGAAGGTATATGCACAGGG
  GGATTCTGGTTTAACCATGAACATTGTTGCTGGAACTCTGAGCATGTCACCTTTGAAGAGAGAGACAAA
  TGTCCAGAGTGGAATAGTTGGTCCCAGCTTATCATCAGCACAGATGAGGGAGCCTTTGCCTACATAGTC
  AATTATTTCATGTACGTCCTCTGGGCTCTCCTATTTGCCTTCCTTGCCGTATCTCTTGTCAAGGTGTTT
  GCGCCTTATGCCTGTGGCTCTGGAATCCCTGAGATAAAAACTATCTTGAGTGGTTTCATTATTAGGGGC
  TATTTGGGTAAGTGGACTCTGGTTATCAAAACCATCACCTTGGTGCTGGCAGTGTCGTCTGGCTTGAGC
  CTGGGCAAAGAGGGCCCTCTAGTGCACGTGGCTTGCTGCTGTGGGAACATCCTGTGCCACTGCTTCAAC
  AAATACAGGAAGAATGAAGCCAAGCGCAGAGAGGTCTTGTCGGCTGCAGCAGCAGCTGGTGTATCTGTA
  GCCTTTGGAGCACCTATAGGTGGAGTATTATTCAGCCTTGAAGAGGTCAGCTACTATTTTCCCCTCAAA
  ACATTGTGGCGTTCATTCTTTGCTGCCTTGGTGGCAGCATTCACTCTACGCTCCATCAATCCATTTGGG
  AACAGCCGCCTGGTACTATTTTATGTGGAGTTTCACACCCCATGGCATCTCTTTGAGCTCGTGCCATTC
  ATTCTGCTGGGCATATTTGGTGGTCTGTGGGGAGCACTGTTTATCCGCACAAACATTGCCTGGTGTCGG
  AAGCGAAAGACCACCCAGTTGGGCAAGTATCCTGTTATAGAGGTACTCGTCGTGACAGCCATCACTGCC
  ATCCTGGCTTTCCCCAATGAATACACTCGGATGAGCACAAGTGAGCTCATTTCTGAGCTGTTTAATGAC
  TGTGGCCTTCTGGACTCCTCCAAGCTCTGTGATTATGAGAACCGTTTCAACACAAGCAAAGGGGGTGAA
  CTGCCTGACAGACCGGCTGGCGTGGGAGTCTACAGTGCAATGTGGCAGCTGGCTTTAACACTCATACTG
  AAAATTGTCATTACTATATTCACCTTTGGCATGAAGATCCCTTCTGGCCTCTTTATCCCTAGCATGGCT
  GTTGGTGCTATAGCAGGTCGACTTCTAGGAGTAGGAATGGAACAGCTGGCTTATTACCACCAGGAATGG
  ACCGTCTTCAATAGCTGGTGTAGTCAGGGAGCTGATTGCATCACCCCCGGCCTTTATGCAATGGTTGGG
  GCTGCAGCCTGCTTAGGTGGGGTGACTCGGATGACTGTTTCTCTTGTTGTCATAATGTTTGAACTGACT
  GGTGGCTTAGAATACATCGTGCCTCTGATGGCTGCAGCCATGACAAGCAAGTGGGTGGCAGATGCTCTT
  GGGCGGGAGGGCATCTATGATGCCCACATCCGTCTCAATGGATACCCCTTTCTTGAAGCCAAAGAAGAG
  TTTGCTCATAAGACCCTGGCAATGGATGTGATGAAACCCCGGAGAAATGATCCTTTGTTGACTGTCCTT
  ACTCAGGACAGTATGACTGTGGAAGATGTAGAGACCATAATCAGTGAAACCACTTACAGTGGCTTCCCA
  GTGGTGGTATCCCGGGAGTCCCAAAGACTTGTGGGCTTTGTCCTCCGAAGAGATCTCATTATTTCAATT
  GAAAATGCTCGAAAGAAACAGGATGGGGTTGTTAGCACTTCCATCATTTATTTCACGGAGCATTCTCCT
  CCATTGCCACCATACACTCCACCCACTCTAAAGCTTCGGAACATCCTCGATCTCAGCCCCTTCACTGTG
  ACTGACCTTACACCCATGGAGATCGTAGTGGATATTTTCCGAAAGCTGGGACTGCGGCAGTGCCTGGTT
  ACACACAACGGGCGATTGCTTGGAATCATTACCAAAAAGGATGTGTTAAAGCATATAGCACAGATGGCG
  AACCAAGATCCTGATTCCATTCTCTTCAAC
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by RC217750
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MDFLEEPIPGVGTYDDFNTIDWVREKSRDRDRHREITNKSKESTWALIHSVSDAFSGWLLMLLIGLLSG
  SLAGLIDISAHWMTDLKEGICTGGFWFNHEHCCWNSEHVTFEERDKCPEWNSWSQLIISTDEGAFAYIV
  NYFMYVLWALLFAFLAVSLVKVFAPYACGSGIPEIKTILSGFIIRGYLGKWTLVIKTITLVLAVSSGLS
  LGKEGPLVHVACCCGNILCHCFNKYRKNEAKRREVLSAAAAAGVSVAFGAPIGGVLFSLEEVSYYFPLK
  TLWRSFFAALVAAFTLRSINPFGNSRLVLFYVEFHTPWHLFELVPFILLGIFGGLWGALFIRTNIAWCR
  KRKTTQLGKYPVIEVLVVTAITAILAFPNEYTRMSTSELISELFNDCGLLDSSKLCDYENRFNTSKGGE
  LPDRPAGVGVYSAMWQLALTLILKIVITIFTFGMKIPSGLFIPSMAVGAIAGRLLGVGMEQLAYYHQEW
  TVFNSWCSQGADCITPGLYAMVGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAAAMTSKWVADAL
  GREGIYDAHIRLNGYPFLEAKEEFAHKTLAMDVMKPRRNDPLLTVLTQDSMTVEDVETIISETTYSGFP
  VVVSRESQRLVGFVLRRDLIISIENARKKQDGVVSTSIIYFTEHSPPLPPYTPPTLKLRNILDLSPFTV
  TDLTPMEIVVDIFRKLGLRQCLVTHNGRLLGIITKKDVLKHIAQMANQDPDSILFN
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000084
• ORF Size: 2238 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000084.3
• RefSeq Size: 3173 bp
• RefSeq ORF: 2241 bp
• Locus ID: 1184
• UniProt ID: P51795
• Cytogenetics: Xp11.23
• Domains: CBS, voltage_CLC
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• MW: 83.1 kDa
• Gene Summary: This gene encodes a member of the ClC family of chloride ion channels and ion transporters. The encoded protein is primarily localized to endosomal membranes and may function to facilitate albumin uptake by the renal proximal tubule. Mutations in this gene have been found in Dent disease and renal tubular disorders complicated by nephrolithiasis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2013]