Chloride Channel 5 (CLCN5) (NM_000084) Human Untagged Clone

CAT#: SC100001

CLCN5 (untagged)-Human chloride channel 5 (CLCN5), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Chloride Channel 5
• Synonyms: ClC-5; CLC5; CLCK2; DENT1; DENTS; hCIC-K2; NPHL1; NPHL2; XLRH; XRN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000084 edited
  GGGCGGCCGCGAATTCGGCACGAGGGGCACGAGGAAGACCTGATGGACATTCCAGCAACC
  GCTATGGATTTCTCCATGAGAGATGATGTTCCTCCCTTAGACCGAGAAGTAGGAGAGGAC
  AAGTCGTACAATGGTGGAGGAATAGGTTCTTCAAATAGGATCATGGACTTCTTGGAGGAG
  CCAATCCCTGGTGTAGGGACCTATGATGATTTCAATACAATTGATTGGGTGAGAGAGAAG
  TCTCGAGACCGGGATAGGCACCGAGAGATTACCAATAAAAGCAAAGAGTCAACATGGGCC
  TTAATTCACAGTGTGAGTGATGCTTTTTCCGGCTGGTTGTTGATGCTCCTTATTGGGCTT
  TTATCAGGTTCGTTAGCTGGTTTGATAGACATCTCTGCTCATTGGATGACAGACTTAAAA
  GAAGGTATATGCACAGGGGGATTCTGGTTTAACCATGAACATTGTTGCTGGAACTCTGAG
  CATGTCACCTTTGAAGAGAGAGACAAATGTCCAGAGTGGAATAGTTGGTCCCAGCTTATC
  ATCAGCACAGATGAGGGAGCCTTTGCCTACATAGTCAATTATTTCATGTACGTCCTCTGG
  GCTCTCCTATTTGCCTTCCTTGCCGTATCTCTTGTCAAGGTGTTTGCGCCTTATGCCTGT
  GGCTCTGGAATCCCTGAGATAAAAACTATCTTGAGTGGTTTCATTATTAGGGGCTATTTG
  GGTAAGTGGACTCTGGTTATCAAAACCATCACCTTGGTGCTGGCAGTGTCGTCTGGCTTG
  AGCCTGGGCAAAGAGGGCCCTCTAGTGCACGTGGCTTGCTGCTGTGGGAACATCCTGTGC
  CACTGCTTCAACAAATACAGGAAGAATGAAGCCAAGCGCAGAGAGGTCTTGTCGGCTGCA
  GCAGCAGCTGGTGTATCTGTAGCCTTTGGAGCACCTATAGGTGGAGTATTATTCAGCCTT
  GAAGAGGTCAGCTACTATTTTCCCCTCAAAACATTGTGGCGTTCATTCTTTGCTGCCTTG
  GTGGCAGCATTCACTCTACGCTCCATCAATCCATTTGGGAACAGCCGCCTGGTACTATTT
  TATGTGGAGTTTCACACCCCATGGCATCTCTTTGAGCTCGTGCCATTCATTCTGCTGGGC
  ATATTTGGTGGTCTGTGGGGAGCACTGTTTATCCGCACAAACATTGCCTGGTGTCGGAAG
  CGAAAGACCACCCAGTTGGGCAAGTATCCTGTTATAGAGGTACTCGTCGTGACAGCCATC
  ACTGCCATCCTGGCTTTCCCCAATGAATACACTCGGATGAGCACAAGTGAGCTCATTTCT
  GAGCTGTTTAATGACTGTGGCCTTCTGGACTCCTCCAAGCTCTGTGATTATGAGAACCGT
  TTCAACACAAGCAAAGGGGGTGAACTGCCTGACAGACCGGCTGGCGTGGGAGTCTACAGT
  GCAATGTGGCAGCTGGCTTTAACACTCATACTGAAAATTGTCATTACTATATTCACCTTT
  GGCATGAAGATCCCTTCTGGCCTCTTTATCCCTAGCATGGCTGTTGGTGCTATAGCAGGT
  CGACTTCTAGGAGTAGGAATGGAACAGCTGGCTTATTACCACCAGGAATGGACCGTCTTC
  AATAGCTGGTGTAGTCAGGGAGCTGATTGCATCACCCCCGGCCTTTATGCAATGGTTGGG
  GCTGCAGCCTGCTTAGGTGGGGTGACTCGGATGACTGTTTCTCTTGTTGTCATAATGTTT
  GAACTGACTGGTGGCTTAGAATACATCGTGCCTCTGATGGCTGCAGCCATGACAAGCAAG
  TGGGTGGCAGATGCTCTTGGGCGGGAGGGCATCTATGATGCCCACATCCGTCTCAATGGA
  TACCCCTTTCTTGAAGCCAAAGAAGAGTTTGCTCATAAGACCCTGGCAATGGATGTGATG
  AAACCCCGGAGAAATGATCCTTTGTTGACTGTCCTTACTCAGGACAGTATGACTGTGGAA
  GATGTAGAGACCATAATCAGTGAAACCACTTACAGTGGCTTCCCAGTGGTGGTATCCCGG
  GAGTCCCAAAGACTTGTGGGCTTTGTCCTCCGAAGAGATCTCATTATTTCAATTGAAAAT
  GCTCGAAAGAAACAGGATGGGGTTGTTAGCACTTCCATCATTTATTTCACGGAGCATTCT
  CCTCCATTGCCACCATACACTCCACCCACTCTAAAGCTTCGGAACATCCTCGATCTCAGC
  CCCTTCACTGTGACTGACCTTACACCCATGGAGATCGTAGTGGATATTTTCCGAAAGCTG
  GGACTGCGGCAGTGCCTGGTTACACACAACGGGCGATTGCTTGGAATCATTACCAAAAAG
  GATGTGTTAAAGCATATAGCACAGATGGCGAACCAAGATCCTGATTCCATTCTCTTCAAC
  TAGAATCATAGAGTTCTGGATGTAAAGCGGGAAGGACATTACAGACCATGGATATGTTGT
  ATTAACTGGGTACCCAAAACACATTTTCCATATTTGGATGGTGAAGTCACATTAGTGTGT
  TGTCTCTTTCCTACAAGTTAACCAGTTGCACTACATAATCTCTGGAAATTAATTTTCTCT
  TTAGGAGAAATTATAGTTAGGCTTCCATGATGTTACATTAGGAAGATATCATGAAAGAAT
  AAATAAGATTGCTATGGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000084 unedited
  TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCACGAGGAAGACC
  TGATGGACATTCCAGCAACCGCTATGGATTTCTCCATGAGAGATGATGTTCCTCCCTTAG
  ACCGAGAAGTAGGAGAGGACAAGTCGTACAATGGTGGAGGAATAGGTTCTTCAAATAGGA
  TCATGGACTTCTTGGAGGAGCCAATCCCTGGTGTAGGGACCTATGATGATTTCAATACAA
  TTGATTGGGTGAGAGAGAAGTCTCGAGACCGGGATAGGCACCGAGAGATTACCAATAAAA
  GCAAAGAGTCAACATGGGCCTTAATTCACAGTGTGAGTGATGCTTTTTCCGGCTGGTTGT
  TGATGCTCCTTATTGGGCTTTTATCAGGTTCGTTAGCTGGTTTGATAGACATCTCTGCTC
  ATTGGATGACAGACTTAAAAGAAGGTATATGCACAGGGGGATTCTGGTTTAACCATGAAC
  ATTGTTGCTGGAACTCTGAGCATGTCACCTTTGAAGAGAGAGACAAATGTCCAGAGTGGA
  ATAGTTGGTCCCAGCTTATCATCAGCACAGATGAGGGAGCCTTTGCCTACATAGTCAATT
  ATTTCATGTACGTCCTCTGGGCTCTCCTATTTGCCTTCCTTGCCGTATCTCTTGTCAAGG
  TGTTTGCGCCTTATGCCTGTGGCTCTGGAATCCCTGAGAAAAAACTATCTGAGTGGTTTA
  TTATTAGGNGCTTTTGGGTAAGTGGACTCTGGTATCAAACCATCACCTGGGTGCTGCAGT
  GTCGTCTGGCTTGAGCCTGGCAAAGAGGGCCTCTANTGCACGTGGCTTGCTGCTGTGGGA
  ACATNCTGTGCCACTGCTTCACAATACAGGAGGATGAAGCAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000084 unedited
  GCCGCGGCCGCAATCTAGAGTCGAGTNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAACCATAACAATCTTATTTATTTTTTCA
  TGAAATCTTCCTAAGGGAACATCTTGGAACCCTAACTTTAATTTTTCCTAAAAAAAAAAT
  TAATTTCCAAAAATTTTGTAGGGCAACGGGTTAACTTGGTGGGGAAAAGAGAAACACCCT
  AATGTGACTTCCCCATCCAAATTTGGAAAATGTGTTTTGGGTACCCAGTTAATACAACAT
  ATCCCTGGTCTGTAATGGCCTTCCCGTTTACATCCAGAACTTTTTGATTCTAGTTGAAAA
  AAATGGAATCAGGATCTTTGGTCCGCCATCTGGGCTAATAGGCTTAAACACATCCTTTTT
  GATAATGATTCCAACCAATTCCCCGTTGTGTTGTACCCAGCCCCTGCCGGAATCCCAGTT
  TTTGGAAAAATTCCCCCTCCAATCCCCATGGGGGTAAGGCCCACCCCCGGGGAAGGGGGC
  TGAAATCGAGGATGTTCCACAAACCTTTAAAAGGGGTGGGATTGTTGGGGGCAATGAAGG
  AAATCCCCCCTAAAAAAATGATGGAAGGCTACCACCCCTTCTGGTTTTTTCTGCCTTTTT
  AATTGAAATAAGGAATCTTTTGGGGGGAAAAACCCCAAATTTTTGGGAACTCCGGGGATA
  CCCCCCTTGGAAACCCCGGAGAGGGTNTTCCCGATTATGGGTTTTAATTTTCCAAAATTT
  TCTGTCTTGGAAAGGAAACCCAAAAGGATTTTTTTCCGGGTTTATCACCTTCTTGCCCGG
  GGTTTTAAAAAACTTTTTTTGTTTAAAAAGGGGTTTCCTTGAACGGGTGGGGCTATTAAT
  CCCCCCCCCAAAACTTTCCCCCCTTTTTTTTTGGGTTGGCCTATAGGCCCTTTTTTTTAA
  CCCCGTGTTTAAATTTTAACAAAAAAAATTTTTGTTCCCCCTTAAGGGGGGCCCACATTT
  TAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000084
• Insert Size: 2241 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000084.1, NP_000075.1
• RefSeq Size: 3173 bp
• RefSeq ORF: 2241 bp
• Locus ID: 1184
• UniProt ID: P51795
• Cytogenetics: Xp11.23
• Domains: CBS, voltage_CLC
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Gene Summary: This gene encodes a member of the ClC family of chloride ion channels and ion transporters. The encoded protein is primarily localized to endosomal membranes and may function to facilitate albumin uptake by the renal proximal tubule. Mutations in this gene have been found in Dent disease and renal tubular disorders complicated by nephrolithiasis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2013]
  Transcript Variant: This variant (3, also known as type 3) lacks several 5' exons but has an alternate 5' exon, and it thus differs in the 5' UTR and uses a downstream start codon, compared to variant 1. The resulting isoform (b) has a shorter N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.