Transcription factor Sp4 (SP4) (NM_003112) Human Tagged ORF Clone

CAT#: RC217532

SP4 (Myc-DDK-tagged)-Human Sp4 transcription factor (SP4)

Product Images

Western blot validation of overexpression lysate (Cat# LY418889) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217532 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SP4
• Synonyms: HF1B; SPR-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217532 representing NM_003112
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGATCAGAAGAAGGAGGAGGAGGAGGAGGCGGCAGCGGCAGCGGCGATGGCTACAGAAGGAGGGA
  AAACCTCTGAGCCAGAGAATAACAATAAAAAACCCAAAACCTCAGGCTCCCAGGACTCTCAGCCCTCTCC
  TCTGGCTTTACTGGCAGCTACTTGCAGCAAAATAGGGACTCCTGGTGAAAATCAAGCAACTGGACAACAA
  CAAATTATTATAGATCCAAGTCAAGGATTGGTGCAACTTCAAAATCAACCACAACAGCTAGAACTGGTAA
  CAACGCAACTTGCTGGAAACGCTTGGCAACTTGTTGCCTCCACTCCTCCTGCTTCAAAAGAGAATAACGT
  TTCTCAACCAGCCTCTAGTTCGTCTAGTTCTTCCAGCAGTAATAACGGGAGTGCATCTCCTACAAAAACT
  AAATCAGGTAATTCTTCCACCCCTGGTCAATTTCAAGTCATACAAGTACAAAATCCAAGTGGTAGTGTAC
  AGTACCAAGTAATTCCACAACTTCAGACAGTGGAAGGTCAACAAATTCAAATCAATCCAACTAGTAGTTC
  ATCTCTACAGGATTTGCAGGGTCAAATTCAGCTCATTTCTGCAGGTAATAATCAAGCTATACTCACAGCT
  GCTAACAGGACAGCTTCTGGGAATATTCTTGCTCAAAACCTGGCAAATCAGACAGTTCCGGTCCAAATTA
  GACCTGGTGTTTCAATACCACTGCAGTTACAGACTCTTCCTGGTACTCAGGCTCAAGTTGTAACAACCCT
  ACCAATTAACATTGGAGGAGTGACTCTAGCTTTGCCAGTGATAAACAACGTGGCTGCCGGAGGAGGGACT
  GGGCAGGTTGGCCAGCCTGCTGCTACTGCTGATAGTGGGACTTCCAATGGGAATCAATTAGTTTCCACAC
  CCACCAACACCACTACTTCTGCCAGTACTATGCCAGAATCTCCCTCCTCCTCCACTACCTGCACAACCAC
  TGCTTCAACGTCTTTGACAAGCAGTGACACATTAGTGAGCTCAGCAGATACTGGCCAGTATGCAAGCACA
  TCAGCCAGTAGTTCTGAACGCACCATTGAAGAATCTCAAACACCTGCTGCTACTGAGTCTGAAGCCCAGA
  GCTCCAGTCAGCTTCAGCCTAATGGAATGCAGAATGCACAGGATCAATCAAATTCTCTTCAGCAGGTGCA
  AATTGTAGGCCAACCTATCTTACAGCAGATCCAGATCCAACAGCCTCAGCAACAGATCATTCAGGCTATT
  CCACCACAGTCGTTTCAACTCCAGTCAGGGCAGACGATTCAGACCATCCAGCAGCAGCCTTTACAGAATG
  TTCAACTTCAAGCAGTAAATCCGACTCAGGTGCTTATCAGGGCTCCAACTTTAACACCTTCAGGGCAAAT
  CAGTTGGCAAACTGTACAGGTTCAGAATATTCAGAGTCTTTCAAATTTGCAAGTTCAGAATGCTGGGTTA
  TCCCAACAATTAACCATCACCCCAGTGTCTTCAAGTGGTGGCACAACTCTTGCTCAGATTGCTCCTGTGG
  CTGTTGCTGGTGCCCCAATAACTTTGAATACTGCCCAGCTTGCATCAGTGCCTAACCTTCAGACAGTGAG
  CGTTGCCAACCTGGGTGCTGCAGGTGTTCAAGTGCAGGGAGTTCCCGTTACAATCACTAGTGTTGCAGGT
  CAGCAGCAAGGACAAGATGGAGTAAAAGTCCAGCAAGCTACTATAGCTCCTGTAACTGTAGCAGTTGGAG
  GAATTGCTAATGCCACGATAGGTGCTGTTAGTCCTGACCAACTCACACAAGTGCATTTGCAGCAAGGCCA
  GCAGACTTCTGATCAAGAGGTACAACCTGGCAAGAGGCTTCGAAGAGTTGCCTGTTCCTGTCCTAATTGT
  AGGGAAGGAGAAGGAAGAGGCAGTAATGAACCAGGAAAAAAGAAGCAGCATATCTGTCATATTGAAGGAT
  GTGGTAAAGTTTATGGCAAAACATCTCATTTACGAGCACATCTTCGCTGGCATACTGGAGAAAGACCTTT
  TATATGCAACTGGATGTTTTGTGGCAAAAGATTCACACGGAGTGATGAGCTCCAGAGACATAGAAGAACC
  CATACAGGTGAAAAGAGATTTGAATGCCCGGAATGTTCTAAAAGGTTTATGCGGAGTGATCATCTCTCCA
  AACATGTCAAAACGCACCAGAATAAAAAAGGTGGTGGGACAGCTCTTGCCATTGTTACCTCGGGAGAACT
  GGACTCATCTGTTACAGAGGTGCTTGGCTCCCCAAGAATTGTCACAGTTGCAGCCATTTCTCAAGATTCG
  AATCCAGCAACTCCCAATGTTTCAACCAACATGGAAGAATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217532 representing NM_003112
  Red=Cloning site Green=Tags(s)
  
  MSDQKKEEEEEAAAAAAMATEGGKTSEPENNNKKPKTSGSQDSQPSPLALLAATCSKIGTPGENQATGQQ
  QIIIDPSQGLVQLQNQPQQLELVTTQLAGNAWQLVASTPPASKENNVSQPASSSSSSSSSNNGSASPTKT
  KSGNSSTPGQFQVIQVQNPSGSVQYQVIPQLQTVEGQQIQINPTSSSSLQDLQGQIQLISAGNNQAILTA
  ANRTASGNILAQNLANQTVPVQIRPGVSIPLQLQTLPGTQAQVVTTLPINIGGVTLALPVINNVAAGGGT
  GQVGQPAATADSGTSNGNQLVSTPTNTTTSASTMPESPSSSTTCTTTASTSLTSSDTLVSSADTGQYAST
  SASSSERTIEESQTPAATESEAQSSSQLQPNGMQNAQDQSNSLQQVQIVGQPILQQIQIQQPQQQIIQAI
  PPQSFQLQSGQTIQTIQQQPLQNVQLQAVNPTQVLIRAPTLTPSGQISWQTVQVQNIQSLSNLQVQNAGL
  SQQLTITPVSSSGGTTLAQIAPVAVAGAPITLNTAQLASVPNLQTVSVANLGAAGVQVQGVPVTITSVAG
  QQQGQDGVKVQQATIAPVTVAVGGIANATIGAVSPDQLTQVHLQQGQQTSDQEVQPGKRLRRVACSCPNC
  REGEGRGSNEPGKKKQHICHIEGCGKVYGKTSHLRAHLRWHTGERPFICNWMFCGKRFTRSDELQRHRRT
  HTGEKRFECPECSKRFMRSDHLSKHVKTHQNKKGGGTALAIVTSGELDSSVTEVLGSPRIVTVAAISQDS
  NPATPNVSTNMEEF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003112
• ORF Size: 2352 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003112.5
• RefSeq Size: 5811 bp
• RefSeq ORF: 2355 bp
• Locus ID: 6671
• UniProt ID: Q02446
• Cytogenetics: 7p15.3
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• MW: 81.8 kDa
• Gene Summary: The protein encoded by this gene is a transcription factor that can bind to the GC promoter region of a variety of genes, including those of the photoreceptor signal transduction system. The encoded protein binds to the same sites in promoter CpG islands as does the transcription factor SP1, although its expression is much more restricted compared to that of SP1. This gene may be involved in bipolar disorder and schizophrenia. [provided by RefSeq, May 2016]