TRIF (TICAM1) (NM_182919) Human Tagged ORF Clone

CAT#: RC217522

TICAM1 (Myc-DDK-tagged)-Human toll-like receptor adaptor molecule 1 (TICAM1)

Product Images

Circular map for RC217522

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TICAM1 (Cat# RC217522, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TICAM1(Cat# TA800679). Positive lysates LY403651 (100ug) and LC403651 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY403651) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217522 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRIF
• Synonyms: IIAE6; MyD88-3; PRVTIRB; TICAM-1; TRIF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217522 representing NM_182919
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTGCACAGGCCCATCACTTCCTAGCGCCTTCGACATTCTAGGTGCAGCAGGCCAGGACAAGCTCT
  TGTATCTGAAGCACAAACTGAAGACCCCACGCCCAGGCTGCCAGGGGCAGGACCTCCTGCATGCCATGGT
  TCTCCTGAAGCTGGGCCAGGAAACTGAGGCCAGGATCTCTCTAGAGGCATTGAAGGCCGATGCGGTGGCC
  CGGCTGGTGGCCCGCCAGTGGGCTGGCGTGGACAGCACCGAGGACCCAGAGGAGCCCCCAGATGTGTCCT
  GGGCTGTGGCCCGCTTGTACCACCTGCTGGCTGAGGAGAAGCTGTGCCCCGCCTCGCTGCGGGACGTGGC
  CTACCAGGAAGCCGTCCGCACCCTCAGCTCCAGGGACGACCACCGGCTGGGGGAACTTCAGGATGAGGCC
  CGAAACCGGTGTGGGTGGGACATTGCTGGGGATCCAGGGAGCATCCGGACGCTCCAGTCCAATCTGGGCT
  GCCTCCCACCATCCTCGGCTTTGCCCTCTGGGACCAGGAGCCTCCCACGCCCCATTGACGGTGTTTCGGA
  CTGGAGCCAAGGGTGCTCCCTGCGATCCACTGGCAGCCCTGCCTCCCTGGCCAGCAACTTGGAAATCAGC
  CAGTCCCCTACCATGCCCTTCCTCAGCCTGCACCGCAGCCCACATGGGCCCAGCAAGCTCTGTGACGACC
  CCCAGGCCAGCTTGGTGCCCGAGCCTGTCCCCGGTGGCTGCCAGGAGCCTGAGGAGATGAGCTGGCCGCC
  ATCGGGGGAGATTGCCAGCCCACCAGAGCTGCCAAGCAGCCCACCTCCTGGGCTTCCCGAAGTGGCCCCA
  GATGCAACCTCCACTGGCCTCCCTGATACCCCCGCAGCTCCAGAAACCAGCACCAACTACCCAGTGGAGT
  GCACCGAGGGGTCTGCAGGCCCCCAGTCTCTCCCCTTGCCTATTCTGGAGCCGGTCAAAAACCCCTGCTC
  TGTCAAAGACCAGACGCCACTCCAACTTTCTGTAGAAGATACCACCTCTCCAAATACCAAGCCGTGCCCA
  CCTACTCCCACCACCCCAGAAACATCCCCTCCTCCTCCTCCTCCTCCTCCTTCATCTACTCCTTGTTCAG
  CTCACCTGACCCCCTCCTCCCTGTTCCCTTCCTCCCTGGAATCATCATCGGAACAGAAATTCTATAACTT
  TGTGATCCTCCACGCCAGGGCAGACGAACACATCGCCCTGCGGGTTCGGGAGAAGCTGGAGGCCCTTGGC
  GTGCCCGACGGGGCCACCTTCTGCGAGGATTTCCAGGTGCCGGGGCGCGGGGAGCTGAGCTGCCTGCAGG
  ACGCCATAGACCACTCAGCTTTCATCATCCTACTTCTCACCTCCAACTTCGACTGTCGCCTGAGCCTGCA
  CCAGGTGAACCAAGCCATGATGAGCAACCTCACGCGACAGGGGTCGCCAGACTGTGTCATCCCCTTCCTG
  CCCCTGGAGAGCTCCCCGGCCCAGCTCAGCTCCGACACGGCCAGCCTGCTCTCCGGGCTGGTGCGGCTGG
  ACGAACACTCCCAGATCTTCGCCAGGAAGGTGGCCAACACCTTCAAGCCCCACAGGCTTCAGGCCCGAAA
  GGCCATGTGGAGGAAGGAACAGGACACCCGAGCCCTGCGGGAACAGAGCCAACACCTGGATGGTGAGCGG
  ATGCAGGCGGCGGCACTGAACGCAGCCTACTCAGCCTACCTCCAGAGCTACTTGTCCTACCAGGCACAGA
  TGGAGCAGCTCCAGGTGGCTTTTGGGAGCCACATGTCATTTGGGACTGGGGCGCCCTATGGGGCTCGAAT
  GCCCTTTGGGGGCCAGGTGCCCCTGGGAGCCCCGCCACCCTTTCCCACTTGGCCGGGGTGCCCGCAGCCG
  CCACCCCTGCACGCATGGCAGGCTGGCACCCCCCCACCGCCCTCCCCACAGCCAGCAGCCTTTCCACAGT
  CACTGCCCTTCCCGCAGTCCCCAGCCTTCCCTACGGCCTCACCCGCACCCCCTCAGAGCCCAGGGCTGCA
  ACCCCTCATTATCCACCACGCACAGATGGTACAGCTGGGGCTGAACAACCACATGTGGAACCAGAGAGGG
  TCCCAGGCGCCCGAGGACAAGACGCAGGAGGCAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217522 representing NM_182919
  Red=Cloning site Green=Tags(s)
  
  MACTGPSLPSAFDILGAAGQDKLLYLKHKLKTPRPGCQGQDLLHAMVLLKLGQETEARISLEALKADAVA
  RLVARQWAGVDSTEDPEEPPDVSWAVARLYHLLAEEKLCPASLRDVAYQEAVRTLSSRDDHRLGELQDEA
  RNRCGWDIAGDPGSIRTLQSNLGCLPPSSALPSGTRSLPRPIDGVSDWSQGCSLRSTGSPASLASNLEIS
  QSPTMPFLSLHRSPHGPSKLCDDPQASLVPEPVPGGCQEPEEMSWPPSGEIASPPELPSSPPPGLPEVAP
  DATSTGLPDTPAAPETSTNYPVECTEGSAGPQSLPLPILEPVKNPCSVKDQTPLQLSVEDTTSPNTKPCP
  PTPTTPETSPPPPPPPPSSTPCSAHLTPSSLFPSSLESSSEQKFYNFVILHARADEHIALRVREKLEALG
  VPDGATFCEDFQVPGRGELSCLQDAIDHSAFIILLLTSNFDCRLSLHQVNQAMMSNLTRQGSPDCVIPFL
  PLESSPAQLSSDTASLLSGLVRLDEHSQIFARKVANTFKPHRLQARKAMWRKEQDTRALREQSQHLDGER
  MQAAALNAAYSAYLQSYLSYQAQMEQLQVAFGSHMSFGTGAPYGARMPFGGQVPLGAPPPFPTWPGCPQP
  PPLHAWQAGTPPPPSPQPAAFPQSLPFPQSPAFPTASPAPPQSPGLQPLIIHHAQMVQLGLNNHMWNQRG
  SQAPEDKTQEAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182919
• ORF Size: 2136 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182919.4
• RefSeq Size: 2460 bp
• RefSeq ORF: 2139 bp
• Locus ID: 148022
• UniProt ID: Q8IUC6
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome
• Protein Pathways: Toll-like receptor signaling pathway
• MW: 76.2 kDa
• Gene Summary: This gene encodes an adaptor protein containing a Toll/interleukin-1 receptor (TIR) homology domain, which is an intracellular signaling domain that mediates protein-protein interactions between the Toll-like receptors (TLRs) and signal-transduction components. This protein is involved in native immunity against invading pathogens. It specifically interacts with toll-like receptor 3, but not with other TLRs, and this association mediates dsRNA induction of interferon-beta through activation of nuclear factor kappa-B, during an antiviral immune response. Mutations in this gene are associated with encephalopathy, acute, infection-induced. [provided by RefSeq, Jul 2020]