PAX1 (NM_006192) Human Tagged ORF Clone

CAT#: RC216709

PAX1 (Myc-DDK-tagged)-Human paired box 1 (PAX1)

Product Images

Circular map for RC216709

• 

  Western blot validation of overexpression lysate (Cat# LY401864) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216709 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PAX1
• Synonyms: HUP48; OFC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216709 representing NM_006192
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAGACGTATGGCGAGGTGAACCAGCTGGGCGGTGTGTTCGTCAACGGCCGCCCCCTGCCCAACG
  CCATCCGCTTGCGCATTGTGGAGCTGGCGCAGCTGGGCATCCGACCCTGTGACATCAGTCGGCAGCTCCG
  CGTATCCCACGGCTGCGTGAGCAAGATCCTGGCGCGCTACAACGAGACCGGCTCCATTCTGCCCGGGGCC
  ATCGGGGGGAGCAAGCCCCGCGTCACCACTCCCAACGTGGTCAAGCACATCCGGGACTACAAGCAAGGAG
  ACCCTGGCATCTTTGCCTGGGAGATCCGCGACCGGCTGCTGGCCGACGGCGTCTGTGACAAGTACAATGT
  GCCTTCGGTGAGCTCCATCAGCCGCATCCTGCGCAACAAGATCGGCAGCCTGGCGCAGCCCGGACCGTAC
  GAGGCAAGTAAGCAGCCGCCGTCGCAGCCTACGCTGCCCTACAACCACATCTACCAGTACCCCTACCCCA
  GTCCCGTGTCGCCCACGGGCGCCAAGATGGGCAGCCACCCCGGGGTCCCGGGCACGGCGGGCCACGTCAG
  CATCCCGCGCTCATGGCCCTCGGCACACTCGGTCAGCAACATCCTGGGCATCCGGACGTTTATGGAGCAA
  ACAGGGGCCCTGGCTGGGAGCGAAGGCACCGCTTACTCTCCCAAGATGGAAGACTGGGCCGGCGTGAACC
  GCACGGCCTTCCCCGCCACCCCCGCAGTGAATGGGCTAGAGAAACCTGCCTTAGAGGCAGACATTAAATA
  CACTCAGTCGGCCTCCACCCTCTCTGCCGTGGGCGGCTTTCTCCCCGCCTGCGCCTACCCGGCCTCCAAC
  CAGCACGGCGTGTACAGCGCCCCGGGCGGCGGCTACCTCGCCCCGGGCCCGCCGTGGCCGCCTGCGCAAG
  GTCCTCCTCTGGCGCCCCCCGGGGCCGGCGTAGCTGTGCATGGCGGGGAACTCGCGGCAGCAATGACCTT
  CAAGCATCCCAGCCGAGAAGGAAGCCTCCCAGCTCCGGCAGCAAGGCCCCGGACGCCCTCAGTAGCTTAC
  ACGGACTGCCCATCCCGGCCTCGACCTCCTAGGGGCAGCTCTCCCCGGACCCGAGCCCGGAGGGAACGGC
  AGGCGGACCCGGGCGCACAGGTCTGCGCGGCGGCCCCGGCAATCGGCACGGGCAGGATCGGAGGACTCGC
  GGAGGAGGAAGCCAGTGCCGGCCCGCGGGGTGCACGCCCAGCCAGCCCCCAGGCCCAGCCCTGCCTCTGG
  CCGGACCCACCACACTTCCTTTATTGGTCTGGGTTTTTAGGCTTCTCTGAACTTGGGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216709 representing NM_006192
  Red=Cloning site Green=Tags(s)
  
  MEQTYGEVNQLGGVFVNGRPLPNAIRLRIVELAQLGIRPCDISRQLRVSHGCVSKILARYNETGSILPGA
  IGGSKPRVTTPNVVKHIRDYKQGDPGIFAWEIRDRLLADGVCDKYNVPSVSSISRILRNKIGSLAQPGPY
  EASKQPPSQPTLPYNHIYQYPYPSPVSPTGAKMGSHPGVPGTAGHVSIPRSWPSAHSVSNILGIRTFMEQ
  TGALAGSEGTAYSPKMEDWAGVNRTAFPATPAVNGLEKPALEADIKYTQSASTLSAVGGFLPACAYPASN
  QHGVYSAPGGGYLAPGPPWPPAQGPPLAPPGAGVAVHGGELAAAMTFKHPSREGSLPAPAARPRTPSVAY
  TDCPSRPRPPRGSSPRTRARRERQADPGAQVCAAAPAIGTGRIGGLAEEEASAGPRGARPASPQAQPCLW
  PDPPHFLYWSGFLGFSELGF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006192
• ORF Size: 1320 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006192.2, NP_006183.1
• RefSeq Size: 2771 bp
• RefSeq ORF: 1605 bp
• Locus ID: 5075
• UniProt ID: P15863
• Cytogenetics: 20p11.22
• MW: 46.1 kDa
• Gene Summary: This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. This gene plays a role in pattern formation during embryogenesis and may be essential for development of the vertebral column. This gene is silenced by methylation in ovarian and cervical cancers and may be a tumor suppressor gene. Mutations in this gene are also associated with vertebral malformations. [provided by RefSeq, Mar 2012]