PAX1 (NM_006192) Human Tagged ORF Clone

CAT#: RC229270

PAX1 (Myc-DDK-tagged)-Human paired box 1 (PAX1)

Product Images

Circular map for RC229270

• 

  Western blot validation of overexpression lysate (Cat# LY432289) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229270 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PAX1
• Synonyms: HUP48; OFC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229270 representing NM_006192
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTTCACCCTGGGCCTGGGGTCGCGGGCGTGGAGAGTGTCCTGGGAGGGGGCAGCAGCGGCGGCGG
  CAGGCCCTGGAGCGGGCGGCAGCGCGCTCCGCTGCCGCGCACAGCGCGTCTCCAGCCCGCGGCTGGGCCG
  CCGCGGCTCTCGGCTCTCGGGCGCCCTCCCTCTATGCCTCTCACGCGGCGGCGGCGGCGCCCAAGCTCTC
  CCGGACTGCGCCGGGCCCAGCCCCGGCCACCCCGGCCACCCCGGCGCCAGGCAGCTGGCCGGCCCGCTCG
  CTATGGAGCAGACGTATGGCGAGGTGAACCAGCTGGGCGGTGTGTTCGTCAACGGCCGCCCCCTGCCCAA
  CGCCATCCGCTTGCGCATTGTGGAGCTGGCGCAGCTGGGCATCCGACCCTGTGACATCAGTCGGCAGCTC
  CGCGTATCCCACGGCTGCGTGAGCAAGATCCTGGCGCGCTACAACGAGACCGGCTCCATTCTGCCCGGGG
  CCATCGGGGGGAGCAAGCCCCGCGTCACCACTCCCAACGTGGTCAAGCACATCCGGGACTACAAGCAAGG
  AGACCCTGGCATCTTTGCCTGGGAGATCCGCGACCGGCTGCTGGCCGACGGCGTCTGTGACAAGTACAAT
  GTGCCTTCGGTGAGCTCCATCAGCCGCATCCTGCGCAACAAGATCGGCAGCCTGGCGCAGCCCGGACCGT
  ACGAGGCAAGTAAGCAGCCGCCGTCGCAGCCTACGCTGCCCTACAACCACATCTACCAGTACCCCTACCC
  CAGTCCCGTGTCGCCCACGGGCGCCAAGATGGGCAGCCACCCCGGGGTCCCGGGCACGGCGGGCCACGTC
  AGCATCCCGCGCTCATGGCCCTCGGCACACTCGGTCAGCAACATCCTGGGCATCCGGACGTTTATGGAGC
  AAACAGGGGCCCTGGCTGGGAGCGAAGGCACCGCTTACTCTCCCAAGATGGAAGACTGGGCCGGCGTGAA
  CCGCACGGCCTTCCCCGCCACCCCCGCAGTGAATGGGCTAGAGAAACCTGCCTTAGAGGCAGACATTAAA
  TACACTCAGTCGGCCTCCACCCTCTCTGCCGTGGGCGGCTTTCTCCCCGCCTGCGCCTACCCGGCCTCCA
  ACCAGCACGGCGTGTACAGCGCCCCGGGCGGCGGCTACCTCGCCCCGGGCCCGCCGTGGCCGCCTGCGCA
  AGGTCCTCCTCTGGCGCCCCCCGGGGCCGGCGTAGCTGTGCATGGCGGGGAACTCGCGGCAGCAATGACC
  TTCAAGCATCCCAGCCGAGAAGGAAGCCTCCCAGCTCCGGCAGCAAGGCCCCGGACGCCCTCAGTAGCTT
  ACACGGACTGCCCATCCCGGCCTCGACCTCCTAGGGGCAGCTCTCCCCGGACCCGAGCCCGGAGGGAACG
  GCAGGCGGACCCGGGCGCACAGGTCTGCGCGGCGGCCCCGGCAATCGGCACGGGCAGGATCGGAGGACTC
  GCGGAGGAGGAAGCCAGTGCCGGCCCGCGGGGTGCACGCCCAGCCAGCCCCCAGGCCCAGCCCTGCCTCT
  GGCCGGACCCACCACACTTCCTTTATTGGTCTGGGTTTTTAGGCTTCTCTGAACTTGGGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229270 representing NM_006192
  Red=Cloning site Green=Tags(s)
  
  MKFTLGLGSRAWRVSWEGAAAAAAGPGAGGSALRCRAQRVSSPRLGRRGSRLSGALPLCLSRGGGGAQAL
  PDCAGPSPGHPGHPGARQLAGPLAMEQTYGEVNQLGGVFVNGRPLPNAIRLRIVELAQLGIRPCDISRQL
  RVSHGCVSKILARYNETGSILPGAIGGSKPRVTTPNVVKHIRDYKQGDPGIFAWEIRDRLLADGVCDKYN
  VPSVSSISRILRNKIGSLAQPGPYEASKQPPSQPTLPYNHIYQYPYPSPVSPTGAKMGSHPGVPGTAGHV
  SIPRSWPSAHSVSNILGIRTFMEQTGALAGSEGTAYSPKMEDWAGVNRTAFPATPAVNGLEKPALEADIK
  YTQSASTLSAVGGFLPACAYPASNQHGVYSAPGGGYLAPGPPWPPAQGPPLAPPGAGVAVHGGELAAAMT
  FKHPSREGSLPAPAARPRTPSVAYTDCPSRPRPPRGSSPRTRARRERQADPGAQVCAAAPAIGTGRIGGL
  AEEEASAGPRGARPASPQAQPCLWPDPPHFLYWSGFLGFSELGF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006192
• ORF Size: 1602 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006192.5
• RefSeq ORF: 1605 bp
• Locus ID: 5075
• UniProt ID: P15863
• Cytogenetics: 20p11.22
• MW: 55.3 kDa
• Gene Summary: This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. This gene plays a role in pattern formation during embryogenesis and may be essential for development of the vertebral column. This gene is silenced by methylation in ovarian and cervical cancers and may be a tumor suppressor gene. Mutations in this gene are also associated with vertebral malformations. [provided by RefSeq, Mar 2012]