NCKAP1 (NM_205842) Human Tagged ORF Clone

CAT#: RC216578

NCKAP1 (Myc-DDK-tagged)-Human NCK-associated protein 1 (NCKAP1), transcript variant 2

Product Images

Circular map for RC216578

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NCKAP1
• Synonyms: HEM2; NAP1; NAP125; p125Nap1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216578 representing NM_205842
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCGCTCAGTGCTGCAGCCCAGTCAGCAGAAGCTGGCGGAGAAGCTCACCATCCTCAACGACCGGG
  GCGTCGGCATGCTCACCCGCCTCTACAACATCAAGAAGCAAGGACAAGTTTGGAAGGCATGTGGAGACCC
  CAAGGCAAAACCATCCTATCTTATCGACAAAAACCTGGAATCTGCTGTGAAATTCATAGTCAGAAAATTC
  CCTGCTGTAGAAACCCGCAACAACAATCAACAGCTTGCACAACTACAGAAAGAAAAATCAGAGATTCTGA
  AAAATCTGGCATTATATTACTTCACATTTGTAGATGTTATGGAATTTAAGGACCATGTTTGTGAATTGCT
  GAATACTATTGACGTTTGCCAAGTCTTCTTTGATATTACTGTAAACTTTGATTTAACAAAGAACTACTTA
  GATTTAATTATAACCTATACAACACTAATGATACTGCTGTCTCGAATTGAAGAAAGGAAGGCAATCATTG
  GATTATACAACTATGCCCATGAAATGACTCATGGAGCAAGTGACAGAGAATACCCACGCCTTGGCCAGAT
  GATTGTGGATTATGAAAACCCTTTAAAGAAGATGATGGAAGAATTTGTACCCCATAGCAAGTCTCTTTCA
  GATGCACTAATTTCTCTTCAAATGGTATATCCTCGAAGGAATCTTTCAGCTGACCAGTGGAGAAATGCCC
  AGTTATTGAGCCTCATCAGTGCACCTAGTACAATGCTTAATCCAGCACAGTCCGACACTATGCCTTGTGA
  ATACCTCTCTTTGGATGCAATGGAAAAGTGGATTATCTTTGGCTTTATTTTGTGCCATGGGATCCTAAAT
  ACTGACGCTACAGCACTGAACCTTTGGAAACTAGCTCTTCAAAGTAGCTCTTGCCTCTCTCTCTTTCGGG
  ATGAAGTTTTCCACATTCACAAAGCTGCAGAAGACTTATTTGTAAACATACGAGGCTATAATAAACGTAT
  TAATGACATAAGAGAATGCAAGGAGGCAGCCGTGTCACATGCTGGTTCAATGCACAGAGAAAGACGCAAG
  TTTTTAAGATCTGCACTGAAGGAATTGGCTACTGTCCTCTCTGATCAACCTGGATTGCTAGGTCCCAAGG
  CACTTTTTGTTTTTATGGCATTATCCTTTGCCCGTGATGAAATCATCTGGCTACTTCGTCATGCAGATAA
  CATGCCAAAGAAGAGTGCAGACGACTTTATAGATAAGCACATTGCTGAATTAATATTTTACATGGAAGAA
  CTTAGAGCACATGTGAGGAAATACGGACCTGTAATGCAGAGGTATTACGTGCAGTACCTTTCTGGCTTTG
  ATGCTGTTGTCCTCAATGAACTCGTGCAGAATCTTTCTGTTTGCCCTGAAGATGAATCAATCATCATGTC
  CTCTTTTGTTAACACTATGACTTCCCTAAGTGTAAAACAAGTTGAAGATGGGGAAGTATTTGATTTCAGA
  GGAATGAGATTAGATTGGTTTAGGTTACAGGCATATACTAGTGTCTCAAAGGCTTCACTTGGCCTTGCAG
  ATCACAGAGAACTTGGAAAGATGATGAATACAATAATTTTTCATACAAAAATGGTAGATTCCTTGGTGGA
  AATGTTGGTGGAAACATCAGATCTCTCCATATTTTGTTTTTATAGTCGTGCTTTTGAGAAGATGTTTCAA
  CAGTGTTTGGAGTTACCCTCTCAATCAAGATACTCAATTGCATTTCCACTACTTTGCACTCATTTTATGA
  GTTGCACGCATGAACTATGTCCAGAAGAGCGACATCATATTGGAGATCGCAGTCTTTCCTTATGTAATAT
  GTTCCTAGATGAAATGGCCAAACAAGCTCGAAATCTCATCACTGATATTTGCACAGAACAGTGTACCCTT
  AGTGACCAGTTGCTACCCAAGCATTGTGCCAAAACTATCAGTCAAGCAGTGAATAAGAAATCAAAAAAGC
  AGACTGGTAAGAAAGGGGAACCTGAAAGGGAGAAACCAGGTGTTGAGAGCATGAGGAAAAACAGGCTGGT
  TGTGACCAACCTTGATAAATTGCACACTGCACTTTCTGAGTTATGCTTCTCTATAAATTATGTACCAAAC
  ATGGTGGTATGGGAACATACCTTTACCCCACGAGAATATTTGACTTCTCATCTGGAAATACGCTTTACCA
  AGTCAATTGTTGGGATGACTATGTATAATCAAGCCACACAGGAAATTGCAAAACCTTCAGAACTTCTAAC
  AAGTGTAAGAGCATACATGACCGTACTCCAGTCAATAGAAAACTATGTGCAGATTGATATTACAAGAGTA
  TTTAATAATGTGCTTCTTCAACAAACACAACATTTAGACAGTCATGGAGAGCCAACCATTACAAGTCTAT
  ACACAAATTGGTATTTGGAAACTTTGTTACGACAAGTCAGCAATGGCCATATAGCATATTTTCCTGCAAT
  GAAAGCGTTTGTGAACTTACCTACAGAAAATGAATTAACATTCAATGCAGAGGAATATTCTGACATATCA
  GAAATGAGGTCATTATCAGAACTACTAGGCCCATATGGTATGAAGTTTCTAAGTGAAAGCCTTATGTGGC
  ATATTTCATCACAAGTTGCTGAACTTAAGAAACTTGTGGTGGAGAATGTTGATGTGTTAACACAAATGAG
  GACCAGCTTTGACAAACCAGACCAGATGGCTGCACTGTTTAAAAGATTATCATCTGTTGACAGTGTCTTG
  AAGAGGATGACAATAATTGGTGTAATTTTATCCTTCCGATCATTGGCACAAGAAGCACTTAGAGATGTCT
  TATCCTACCACATTCCTTTTCTTGTAAGTTCAATTGAAGATTTTAAGGATCACATTCCAAGGGAAACTGA
  TATGAAGGTTGCAATGAATGTGTATGAGTTATCATCAGCTGCCGGATTACCTTGTGAGATTGATCCTGCA
  TTGGTCGTAGCTCTTTCTTCACAAAAATCGGAAAACATTAGTCCAGAAGAAGAGTATAAAATTGCCTGCC
  TTCTCATGGTGTTTGTGGCAGTTTCTTTGCCAACACTGGCCAGTAATGTGATGTCTCAGTACAGCCCTGC
  TATAGAAGGGCATTGCAACAACATACATTGCTTGGCCAAAGCCATCAACCAGATTGCTGCAGCTTTGTTT
  ACAATTCACAAAGGAAGCATTGAAGACCGTCTTAAAGAATTTCTGGCGCTTGCATCCTCCAGTCTACTGA
  AAATTGGCCAGGAGACAGATAAAACTACAACAAGAAATAGAGAATCTGTTTATTTACTGCTAGATATGAT
  TGTACAAGAATCTCCATTCCTTACAATGGATCTTTTGGAATCTTGTTTTCCTTATGTCTTGCTGAGAAAT
  GCATACCATGCTGTCTACAAACAAAGTGTTACATCTTCTGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216578 representing NM_205842
  Red=Cloning site Green=Tags(s)
  
  MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKF
  PAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYL
  DLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLS
  DALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILN
  TDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRK
  FLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEE
  LRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFR
  GMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQ
  QCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTL
  SDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPN
  MVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRV
  FNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDIS
  EMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVL
  KRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPA
  LVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALF
  TIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRN
  AYHAVYKQSVTSSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_205842
• ORF Size: 3402 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_205842.3
• RefSeq Size: 4637 bp
• RefSeq ORF: 3405 bp
• Locus ID: 10787
• UniProt ID: Q9Y2A7
• Cytogenetics: 2q32.1
• Protein Families: Druggable Genome
• Protein Pathways: Regulation of actin cytoskeleton
• MW: 129.3 kDa
• Gene Summary: Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes.[UniProtKB/Swiss-Prot Function]